FastQCFastQC Report
Fri 30 Jun 2023
SRR2060685.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060685.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29053643
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC27109529.33085052363313No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC22400277.709969452023624No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA13214934.548458862800786No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG8678902.987198541676856No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA7033592.4208977855203906No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA6629742.2818962840563577No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA5789651.9927449373560484No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT2664080.9169521357442163Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2471880.8507986416712011No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC2471290.8505955690307064Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC2414640.8310971536340553Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1710560.5887592134315136No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1542160.5307974631615044No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG1383130.47606078177528377No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC1306820.4497955729682505No Hit
TGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGT1233810.42466619418432316No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG1182520.407012642097929No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1109540.3818935890414844No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC1027160.3535391413737685No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT999910.3441599389102427No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC927650.3192887033133848No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC834730.28730648339005194Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG834370.2871825746602586No Hit
TCGCTGCGATCTATTGAAAGTCAGATCGGAAGAGCACACGTCTGAACTCC798450.2748192369542091Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA788380.2713532344291557No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT721140.24820983723108322No Hit
TCGACCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC718630.24734591803169054No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC687740.23671386063358735No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC683170.23514090814704372No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG682380.2348689973233305No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAAGAGCACACGT682080.2347657400485027No Hit
ACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGA681920.23471066950192784TruSeq Adapter, Index 2 (100% over 36bp)
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG674410.23212579572207173No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC663240.22828118318931637No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC660310.22727270380516482No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG659660.22704897970970456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC650960.22405451873969817No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG631890.21749079796981055No Hit
TCGCTGCGATCTATTGAAAGTAGATCGGAAGAGCACACGTCTGAACTCCA604310.2079980125039741Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT594380.20458019670717367No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC587110.20207792874717984No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAT582770.20058414017133758TruSeq Adapter, Index 2 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC578540.19912821259626548No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA576470.1984157373999536No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCAGATCGGAAGAGCACACG569850.19613719353541997No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT564430.1942716787701976No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC543860.18719167162617095No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG539840.18580802414347833No Hit
CCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG526020.18105130568307734TruSeq Adapter, Index 2 (100% over 35bp)
TCGCTGCGATCTATTGAAAGAGATCGGAAGAGCACACGTCTGAACTCCAG491370.1691250904404656Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCTATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC450670.15511652015549307No Hit
ATGGTTAGCACTCTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA443020.15248345964738397Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC440510.15161954044799134Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC422930.1455686641430818No Hit
GCCAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG420060.1445808362138958No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC401240.13810316317303134No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA400590.1378794390775711No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC389740.1341449676379654No Hit
CTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACG374460.1288857304400691No Hit
TCGCTGCGATCTATTGAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT370350.12747110577492812Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC368370.1267896077610646No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGT350520.12064579990881007No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG346160.11914512751464593No Hit
CGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA340390.11715914592879109Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTC334940.11528330543608593No Hit
AGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC330920.11389965795339332No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT330360.1137069110403814No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG321770.11075031107114519No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC320370.11026844378861543No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA314140.10812413438135796No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC313580.10793138746834606No Hit
TCGCTGTGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC312560.10758031273393151No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT304310.1047407376761668Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA300170.10331578728354307No Hit
AAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATCGGAAGAGCACACGT297610.10243465853834578No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC293970.10118180360376837No Hit
GATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGA293840.10113705878467634Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCCTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA291180.10022151094786977No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)