FastQCFastQC Report
Sat 1 Jul 2023
SRR2060686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64964205
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC37172605.722012606788615No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG28666834.4127115847873455No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT24946283.8400038913737187No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG19723153.0360026725486753No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG13595182.0927185978801712No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCAGATCGGAAGAGCACACG10177891.5666919959999512No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG10074291.5507447524371305No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT8765771.3493230618307421No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA7478271.151136999213644No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG7460671.1484278149790335No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT6833171.0518361611598264No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT6298640.9695554651981041No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC6297240.9693399619067147No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC5365210.8258717242826261No Hit
GTTTCCGTAGTGTAGTGGTTATCACGCTCGCCAGATCGGAAGAGCACACG4229640.6510723867089576No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC4213030.648515594087544No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTGAGATCGGAAGAGCACA3694740.5687347363059395No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC3317260.5106288917104427No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT3273540.5038990317821945No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTAGATCGGAAGAGCACAC3199730.492537390398297No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC3199270.4924665821739833No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC3088540.47542181113430076No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC2991530.460488972350235No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTGAGATCGGAAGAGCACAC2722610.419093868692767No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2586820.39819158873721305No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC2547340.3921143959200301Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG2365470.3641189790593143No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC2353950.3623456948330238No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC2240950.3449515005994455No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG2137560.3290365825303334No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG2116150.32574092148129885No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2098080.3229593897131505No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC1979040.30463545270814907No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAAGAGCACACGT1928390.29683885148752304No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC1900060.29247798845533474No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCACCAGATCGGAAGAGC1882640.28979651178676014No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1821450.28037747864381624No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1806520.27807928997207No Hit
GAATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1665820.25642120918742867No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1648300.25372433942661193No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCTAGATCGGAAGAGCACAC1561100.24030156299149663No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTAGATCGGAAGAGCACAC1549330.23848979603460088No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTGAGATCGGAAGAGCACA1530130.2355343223241168No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCT1497350.23048846668715486Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTAGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1487420.22895993262751385No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1484650.22853354397240755Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAT1451940.22349846350001512TruSeq Adapter, Index 3 (100% over 41bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGT1416020.21796926476665113No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1407260.2166208298862427Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC1343430.2067954191081073No Hit
GCCAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1341300.20646754624335048No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1332510.20511449343526947No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1260480.19402684909328147No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC1191100.1833471216957092No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG1186460.18263288221567553No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG1169770.18006377512046826No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1155590.1778810346405378No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG1155010.1777917547055336No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA1070170.16473225524733198No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCAGATCGGAAGAGCACA1066100.1641057563930783No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT1051540.16186452216262787No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT1020470.15708188840300594No Hit
GGCTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCT987360.15198523556164506Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCAGATCGGAAGAGCAC963030.148240096219141No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTCCCAGATCGGAAGAGCAC921340.14182271606340752No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCAGATCGGAAGAGCACACG917920.14129627230872757No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC872020.13423084296960147No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC864580.1330855969067889No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC848080.1305457366868416No Hit
AGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC844270.12995925987241744No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACACG832110.1280874598557775No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCAGATCGGAAGAGCACACGT831160.1279412254794775No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC824900.12697761790512174No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACAGATCGGAAGAGCACACGT816290.12565227266307652No Hit
TCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT804580.12384974156152609No Hit
TCCATGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG797510.12276144994000929No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA796120.12254748595784401No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT788190.12132681374304512Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG782720.12048481159740197No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA774150.11916562359225362No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG772560.11892087342560413No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCTCCAGATCGGAAGAGCACA760870.11712142094250211No Hit
GGCTCGTTCGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG752920.1158976701092548No Hit
TGTATGAGGCCCCGGGTTCGATCCCCGGCATCTCCAGATCGGAAGAGCAC751500.11567908819941689No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA751420.11566677372562321No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCAGATCGGAAGAGCACA744340.1145769427948822No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC731430.11258969458642648No Hit
GGGGGTATAGCTCAGTGGTAGAGCATTTGACTGAGATCGGAAGAGCACAC702190.10808875441483508No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGAGATCGGAAGAGCACA695130.10700200210254247No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC681940.10497165323580886No Hit
ATGGTTAGCACTCTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA680600.10476538579976466Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCTCGTTGGTCTAGGGGTATGATTCTCGGTTAGATCGGAAGAGCACACG674200.10378022789626995No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACAGATCGGAAGAGCACACG674080.10376175618557944No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC673130.10361552180927942No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)