FastQCFastQC Report
Fri 30 Jun 2023
SRR2060687.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060687.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21767084
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC326609415.004738347130006No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC17116587.863515388648292No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA14923766.856113570379938No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA9383414.310825464724628No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG3965581.8218241818702037No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA3675721.6886598131380393No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2258211.0374425899215531No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2185431.0040067838209288No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA2049000.941329578183279No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1276820.5865829341219981Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC1175370.5399758644750027Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1163250.5344078242175204No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1152470.5294553923713438No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1110810.5103164025094037No Hit
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG1084610.49827987984058864No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1055940.4851086162942175No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1047150.4810704088797562No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA983020.45160849289688965No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT909870.41800270536926304No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC824760.3789023830661011No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGATCGGAAGAGCACA711610.32692022505173407No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG702320.3226523130061886No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC688320.3162205833358295Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC684260.3143553817314253No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG672010.30872761826986106No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC609480.28000075710646405No Hit
TCGACCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC556750.2557761067123185No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG555730.2553075092649066TruSeq Adapter, Index 4 (100% over 49bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT533740.245205099589821No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC524860.24112554534176464No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG521130.23941194879387612No Hit
TCTATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC519260.23855285347362098No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAGATCGGAAGAGCACAC509980.2342895355206972No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT488330.22434332499474893Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA486060.22330046596962644No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA486030.22328668369176138No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAGATCGGAAGAGCACAC474500.21798969489895845No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT446060.204924095482886No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG437190.20084913532745133No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC419510.19272677957231202No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC391230.17973468563818654No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG382600.1757699837056723No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA369780.169880356964672No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC359750.16527248206512182No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG352710.1620382408594555No Hit
CGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA340430.15639669512002619Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC323870.14878887773851565No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG321200.1475622550085257No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAGATCGGAAGAGCACACG321010.1474749672487137No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC313670.14410290326439684Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC312180.14341838346376576No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC310520.14265576408856603No Hit
TCCCGTGGATCGCCTCAGCAGATCGGAAGAGCACACGTCTGAACTCCAGT308430.14169559873063384Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCGTGCATTTATCAGATCAAAACCAACCCGGAGATCGGAAGAGCACACG307080.14107539622670634No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC292100.13419344547942205No Hit
AGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAGATCGGAAGAGCACAC289440.1329714168420538No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAAGAGCACACGT288920.1327325240257262No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGT286920.13181370550138918No Hit
TCCTACTTGGATAACTGTGGTAATTCTAGAGCTAGATCGGAAGAGCACAC276330.12694856141502464No Hit
TTGGAATGAGTCCACTTTAAATCCTTTAACGAGGAGATCGGAAGAGCACA269860.12397618348879437No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAGATCGGAAGAGCACACG266240.12231312195974436No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA265390.12192262408690113No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAAAGATCGGAAGAGCACA262990.1208200418576967No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT260850.11983690603665609No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA255410.11733771965045939No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC253060.11625810788436339No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG247150.11354299914494749Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA243550.11188912580114084No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACAC240850.11064872079328586No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC240650.11055683894085216No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA239520.11003770647460175No Hit
TCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC236770.10877433100363834TruSeq Adapter, Index 4 (100% over 37bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT224880.10331195487645474No Hit
TGGGGGGAACCTCCGCGTCGGAGATCGGAAGAGCACACGTCTGAACTCCA218530.10039470606168469Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)