FastQCFastQC Report
Fri 30 Jun 2023
SRR2060688.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060688.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24180440
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT255732510.576006888212126No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC11635224.81183138106668No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT8418423.4814999230783226No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA7223222.987216113519853No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG5807572.4017635741946797No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG4066551.6817518622489913No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT4058291.678335878089894No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG3708241.5335701087325126No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAGATCGGAAGAGCACACGTC3172401.3119695092396995No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT2926541.210292285831027No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2785921.1521378436455252No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGTC2719821.1248016992246626No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2676781.1070021885457833No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT2061600.8525899445998502No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTC2008280.8305390638052905No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT1916110.7924214778556553No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCCAGATCGGAAGAGCACACG1848900.7646262847160763No Hit
GAATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1762900.7290603479506577No Hit
GCATTGGTAGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1698310.7023486752102113No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1502520.6213782710322889No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC1481820.6128176327643335No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACG1481300.612602582914124No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT1460540.6040171312019136No Hit
GTTTCCGTAGTGTAGTGGTTATCACGCTCGCCAGATCGGAAGAGCACACG1342170.555064341260953No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT1056720.43701438021806055No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT993290.410782434066543No Hit
CTCGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATC852300.3524749756414689TruSeq Adapter, Index 5 (100% over 42bp)
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC790680.3269915683916422No Hit
TCATTGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT761810.315052166130972No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC739340.3057595312574957Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCAGATCGGAAGAGCACACGT720590.29800532992782597No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGAGATCGGAAGAGCACAC719720.2976455349861293No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTAGATCGGAAGAGCACAC648170.2680555027121095No Hit
GAATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT597670.24717085379753223No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG592560.24505757546181955No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCTAGATCGGAAGAGCACACG590780.24432144328225625No Hit
GGTTCCATGGTGTAATGGTTAGCACTCTGGAAGATCGGAAGAGCACACGT580340.2400039039818961No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAGATCGGAAGAGCACA578960.23943319476403244No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG578000.23903617965595333No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG570330.23586419436536307No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC564480.23344488355050613No Hit
GCACGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT543470.2247560424872335No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT535680.22153443030813336Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACACG519360.2147851734707888No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCTAGATCGGAAGAGCACACG504070.20846188075982075No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA499860.20672080408793223No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG495580.20495077839774628No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC430390.17799097121475044No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCTAGATCGGAAGAGCACACGT425250.1758652861569103No Hit
CTCGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGAT423090.17497200216373235TruSeq Adapter, Index 5 (100% over 41bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA421440.1742896324467214No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC407460.16850809993531962No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCAC387500.1602534941465085No Hit
TGTGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAAC386700.15992264822310925Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG366370.15151502619472598No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGGCAGATCGGAAGAGCACACGT364280.15065069121984548No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC356040.14724297820883325No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCAAGATCGGAAGAGCACACGT354270.14651098160331244No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAAGATCGGAAGAGCACACGT346280.1432066579433625No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTAGATCGGAAGAGCACAC340690.14089487205361026No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGTCT338830.14012565528170703Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGTC335780.13886430519874743No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA334010.1381323085932266No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC332150.13736309182132336Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCCACATGGTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGT329260.1361679109230436No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAAGATCGGAAGAGCACAC326790.13514642413454842No Hit
GTTTCCGTAGTGTAGTGGTTATCACATTCGCCAGATCGGAAGAGCACACG326100.13486106952561658No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGAGATCGGAAGAGCACACGTC325940.13479490034093672No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCAGATCGGAAGAGCACACGTC324130.13404636143924592No Hit
TGTATGAGGCCCCGGGTTCGATCCCCGGCATCTCCAGATCGGAAGAGCAC318230.13160637275417653No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT304270.12583311139085973No Hit
TCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCAGATCGGAAGAGCACAC301450.12466687951087738No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCAGATCGGAAGAGCACACGT297620.12308295465260351No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTAGATCGGAAGAGCACACG291400.12051062759817441No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC286410.1184469761509716No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCT281330.11634610453738642Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATTGGTGGTTCAATGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT273920.11328164417190092No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACAGATCGGAAGAGCACACG269830.11159019438852229No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCAGATCGGAAGAGCACACG264020.10918742586983529No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGT261280.1080542785821929No Hit
GGCTTGGTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCT258830.1070410629417827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG257720.10658201422306625No Hit
TCATGGGTGGTTCAGTGGTAGAATTCTCGCCAGATCGGAAGAGCACACGT256450.10605679631966994No Hit
CGCGTCGGGGTCGGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA256430.10604852517158497Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA256370.10602371172733004No Hit
GGCTCGTTGGTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTC254870.10540337562095643No Hit
GTTTCTGTAGTGTAGTGGTTATCACGCTCGCTAGATCGGAAGAGCACACG253500.10483680197713524No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC253120.1046796501635206No Hit
TCCATGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG249390.10313708104567162No Hit
TCCCTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTG248010.10256637182780794Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA246750.10204528949845412No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCTAGATCGGAAGAGCACACGT242740.10038692430741543No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)