FastQCFastQC Report
Fri 30 Jun 2023
SRR2060689.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060689.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10236103
Total Bases522 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2996732.92760828999083No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT1387711.355701481315692No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1319621.2891820256204924No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC1318511.2880976285604004No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1236301.2077838607133984No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC1208971.1810842466122118No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC918550.8973629905834282No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA552680.5399320424970323No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC460800.45017132008148025No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA453660.44319600926250935No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA366990.3585251144893716No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG339030.33121003178651093No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC323290.3158330860875472No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC318770.31141734310410907No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC264120.25802788424461925Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG251850.2460409005263038No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA232510.2271469913892035No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC226760.22152961923106873No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTAGATCGGAAGAGCACACG219580.214515231040563No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC196710.19217274386551209No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA174910.17087557637901846No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT163630.15985575760619056Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACA163130.15936729046200493No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAGATCGGAAGAGCAC159200.15552793870870585No Hit
GCCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT144450.14111815795522964No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT141550.13828504851895299No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACACGT139780.13655587482853582No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA139330.13611625439876876No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG135410.1322866719883534No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA132740.1296782574384021No Hit
ACAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC131830.12878924723598423No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGCAGATCGGAAGAGCACACGT130900.12788069834779894No Hit
TGTGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC129030.1260538312285447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC124320.12145247073031604No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG117720.11500470442706566No Hit
ACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA113880.11125327675971998Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG110280.10773631332158343No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTCAGATCGGAAGAGCACAC107680.10519628417181813No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)