FastQCFastQC Report
Fri 30 Jun 2023
SRR2060691.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060691.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19996790
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC15240497.6214682456534275No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA11044785.523276485875983No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC4785552.393159102035877No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG4328902.1647974499907234No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA3794461.8975345542959643No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA3718421.8595084511064026No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC2963321.481897844604059No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA2879521.4399911185745313No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2461621.2310075767160629No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2149831.0750875515520242No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG2116161.0582498491007806TruSeq Adapter, Index 4 (100% over 49bp)
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1771170.8857271592090531No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1649840.8250524209135567No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1223720.6119582192941967No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1119750.5599648743623352No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC968530.48434273700929No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA942290.4712206309112612No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC880770.44045569313874877No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA852600.42636843213335734No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG807920.404024845987781No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC730420.3652686256144111No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA638090.3190962149424983No Hit
CCCAAAGTCGGTGAGTTAACTTCGGAGACCATTGAGATCGGAAGAGCACA637250.3186761475216772No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC534310.26719788526058436No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC528760.2644224398015882No Hit
TAGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC513010.2565461756611936No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA508760.2544208345439443No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC505770.2529255945579265No Hit
GGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA490920.24549940265412598No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA467300.2336875068448486No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA458670.22937181417617528No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC445060.22256572179834866No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA413260.20666316943869492No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC396990.19852686356160165No Hit
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC380840.19045056731605423No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC379800.18993048384265673Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA370230.18514471572687416No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT354410.17723344596807786No Hit
TGGGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA346310.1731827958387321No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC342750.1714025101028715No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG340940.17049736482705474No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC338840.16944719627500213No Hit
CCCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA321970.16101084224017956No Hit
TCTATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC310540.15529492483543608No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA309710.15487985821724387No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA291510.14577839743278798No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC291460.14575339341964386No Hit
TATATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC288180.14411313015739025No Hit
GATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC281670.14085760764602717No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT280230.14013749206747683Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC275640.13784212366084758No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC270370.1352067006754584No Hit
GCGCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG263730.13188616772992065No Hit
TGCCTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA252230.12613524470677545No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC245260.12264968527448655No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA241780.12090940595965653No Hit
GACAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG238140.11908911380276535No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA236810.11842400705313202TruSeq Adapter, Index 4 (100% over 36bp)
GCCAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG234010.11702378231706188No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG221480.11075777662314802No Hit
AGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA220150.1100926698735147No Hit
TGCGTGCATTTATCAGCTCAAAACCAACCCGGTGAGATCGGAAGAGCACA213240.10663711525699875No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC212300.10616703980988948No Hit
TACCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG203430.10173132787812443No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)