FastQCFastQC Report
Fri 30 Jun 2023
SRR2060692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10192777
Total Bases519.8 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC6660566.53458816964209No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG3002972.9461745312391314No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC2172612.1315192120851854No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2160152.1192948692981313No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1985121.9475752290077573No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1612521.582022249677394No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1448881.421477189189953No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1203471.1807086528038433No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC1050641.0307691417167273No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA934440.9167668438149877No Hit
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG807000.7917371291454722No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC779900.7651496741270805No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG758780.7444291187769536TruSeq Adapter, Index 5 (100% over 49bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC671230.6585349605902296No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT594750.5835014343981036Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA551450.5410203715827394No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG503060.49354557644104247No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC490320.48104652932169517No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC462500.45375269173454885No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA398500.39096313006749783No Hit
GGGAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA370260.3632572359819115TruSeq Adapter, Index 5 (100% over 36bp)
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC367030.3600883252915275No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT341850.33538455712314713No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA338950.3325394051101088No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA333640.3273298336655457No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA315450.3094838629354885No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA287570.2821311601342794No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC263340.25835942452189425No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATAGATCGGAAGAGCACACG253930.24912739678303567No Hit
TTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACAC252450.24767538816948512No Hit
CCCAAAGTCGGTGAGTTAACTTCGGAGACCATTGAGATCGGAAGAGCACA250490.2457524578434317No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC244980.240346669018659No Hit
AGCGGAGTAGAGCAGTTGGAAGCTCGTTGGGCAGATCGGAAGAGCACACG235410.23095766737563275No Hit
TAGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC228230.2239134634261105No Hit
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA222210.21800732028180347No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC221240.21705566598778725No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC220690.216516068192211No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC217680.21356299662005754No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG214800.21073746634504023No Hit
GGTAGCGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA204160.20029870171789296TruSeq Adapter, Index 5 (100% over 36bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT202930.19909196482960434No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA202210.19838558226085No Hit
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC199380.19560910633088507No Hit
TCGAGGCCCTGTAATTGGAATGAGTCCACTTTAGATCGGAAGAGCACACG195890.19218511304622873No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACG195000.1913119457042963No Hit
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCTAGATCGGAAGAGCACAC186660.1831296809495587No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA181610.17817519209926796No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACA174970.1716607750763114No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCAGATCGGAAGAGCACACG172090.16883524480129408No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTAGATCGGAAGAGCACACG167010.1638513233439719No Hit
GACAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG166130.16298796687104997No Hit
GCGCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG158190.15519813687673145No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAGATCGGAAGAGCACA156180.1532261522056256No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA155470.15252958050588178No Hit
GCCAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG154140.15122473492748836No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTAGATCGGAAGAGCACAC154130.1512149240584779No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC151340.14847769160455487No Hit
TCTATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC146500.14372923100348411No Hit
AGAACGAAAGTCGGAGGTTCGAAGACGATCAGAGATCGGAAGAGCACACG145540.14278738757847836No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC142820.1401188312076287Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC142480.13978526166127248Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG141720.13903963561647625No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA135400.13283916640185495No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG133890.13135772518127298No Hit
TATATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC133300.13077888390965484No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAGATCGGAAGAGCAC131110.12863030359636043No Hit
GATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTG128830.12639342546197174Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG127780.12536328421587167No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC126930.12452936034998117No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAGATCGGAAGAGCACAC125890.12350902997289158No Hit
GATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC125110.1227437821900744No Hit
TGCCGTGATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACAC124960.12259661915491726No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA124700.12234153656064486No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC121120.11882924545489419Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC115340.11315856316683863No Hit
AGCTGTACTGACTATAATCAAACTCCGAATACCATTAGATCGGAAGAGCA115210.11303102186970243No Hit
GGCTCTGTAGCTCAGTTGGTTAGAGCGTTCGGTAGATCGGAAGAGCACAC112650.11051943940302039No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC110760.1086651851600403Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA110250.10816483084050597No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACTAGATCGGAAGAGCA106420.1044072680094934No Hit
GGAGAAGTAGCTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACA105670.10367145283370763TruSeq Adapter, Index 5 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)