FastQCFastQC Report
Fri 30 Jun 2023
SRR2060693.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060693.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24131402
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC23237239.629457086662432No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA9061033.7548709353894973No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC7280703.0171060927168676No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG6352822.6325946581968176No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC5976342.4765821728882558No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA5077202.1039805312596425No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC3705651.535613222969805No Hit
TCGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA3640921.5087892531068026No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC3100531.2848528237190693No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC2645661.0963556945427373No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1951860.8088464980194686No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG1833840.7599392691730054TruSeq Adapter, Index 6 (100% over 49bp)
GACCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG1708860.708147831609618No Hit
TCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA1626950.6742045074712194No Hit
TCCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA1524320.6316748608307134No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAGATCGGAAGAGCACA1474130.6108762350401357No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1418050.5876368061830805No Hit
TCTCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA1184700.490937078583333No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG1148630.4759897497874347No Hit
TATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAACT1100890.4562063986170385Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC1059060.4388721384692029No Hit
TAGCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA839310.34780822100597386No Hit
TCGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC797660.33054855246288634No Hit
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACAGATCGGAAGAGCAC757360.3138483209554091No Hit
TAGATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC742500.3076903695856544No Hit
TAGCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA643900.26683074609589613No Hit
TATCTGCGATCTATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACA608870.25231439101631975No Hit
ACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGATCGGAAGAGCACAC540890.22414362828981094No Hit
GCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC529500.21942363730047676No Hit
CCCAAAGTCGGTGAGTTAACTTCGGAGACCATTGAGATCGGAAGAGCACA506560.20991735167314357No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACGT496650.20581066943395993No Hit
ACCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC494200.20479539481377834No Hit
TCTATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC470280.19488299933837247No Hit
TATCCCGTCACGTTGAACGCACGTTCGTGTGGAACCAGATCGGAAGAGCA457480.18957870744517868No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACA455660.1888245034416152No Hit
GGCTCTGTAGCTCAGTAGGTAGAGCAACGGTAGATCGGAAGAGCACACGT439560.1821526987947074No Hit
AGAACTGGTGCGGACCAGGGGAATCCGACTGTTAGATCGGAAGAGCACAC436310.1808059059312012No Hit
GATCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC433980.17984035904751824No Hit
TATATGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC430520.1784065426451393No Hit
GGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA413540.17137006793057447No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC398510.1651416689341133No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC393290.16297851239642022No Hit
CTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCACACGTCTGAAC375440.15558151159223985Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGCGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG350280.14515526283968083No Hit
TGGGGGGAACCTCCGCGTCGGTGAGATCGGAAGAGCACACGTCTGAACTC347050.14381675793225773Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGCGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCAC345570.1432034491821072No Hit
TATTGAAAGTCAGCCCTCGACACAGATCGGAAGAGCACACGTCTGAACTC340470.1410900203809128Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTCATCACGCTCGCCAGATCGGAAGAGCACACG336270.1393495496034586No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGTAAGATCGGAAGAGCACA333820.13833427498327697No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACAC320130.13266116904438457No Hit
AGCAGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCAC308490.1278375786040115No Hit
GCTCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC302520.1253636237132016No Hit
GACAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG301230.12482905054584065No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCA287800.11926368803602874No Hit
TACCTGGTGGTCTAGTGGTTAGGATTCGGCGCAGATCGGAAGAGCACACG287240.11903162526570152No Hit
TCGCTGCGATCTATTGAACGTCAGCCCTCGACACAAGATCGGAAGAGCAC281680.11672757347459546No Hit
GCCAGGCTAGCTCAGTCGGTAGAGCATGAGACAGATCGGAAGAGCACACG268500.11126581041582251No Hit
CCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGATCGGAAGAGCAC267090.11068150951196287No Hit
AGCCGCCTGGATACCGCAGCTAGGAATAATGGAAGATCGGAAGAGCACAC267080.11067736553392131No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACAGATCGGAAGAGCACACG259650.107598389849044No Hit
AGCCGATTTAGCTCAGCGGTAGAGCAGCTGGCAGATCGGAAGAGCACACG254320.10538964955289376No Hit
TGGGTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA253900.10521560247514834No Hit
TGCCTGCATTTATCAGATCAAAACCAACCCGGTGAGATCGGAAGAGCACA252040.10444482255941864No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)