FastQCFastQC Report
Fri 29 Sept 2023
SRR2060716.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060716.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314737040
Total Bases16 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC8220882126.119843091871232RNA PCR Primer, Index 6 (100% over 50bp)
CGATGGATACTAGGCGCTGTGCGTTGGAATTCTCGGGTGCCAAGGAACTC32225261.0238788545510882RNA PCR Primer, Index 1 (100% over 26bp)
AACGATGGATACTAGGCGCTGTGCGTTGGAATTCTCGGGTGCCAAGGAAC17101010.5433427854567101RNA PCR Primer, Index 1 (100% over 24bp)
TATTCTGGTGTCCTAGGCGTAGAGGATGGAATTCTCGGGTGCCAAGGAAC16678680.5299242821880767RNA PCR Primer, Index 1 (100% over 24bp)
ACGATGGATACTAGGCGCTGTGCGTTGGAATTCTCGGGTGCCAAGGAACT15384760.4888131374686628RNA PCR Primer, Index 1 (100% over 25bp)
CGATGCCGACCAGGGATCAGCGGATGTTGGAATTCTCGGGTGCCAAGGAA14339280.455595566381383RNA PCR Primer, Index 1 (100% over 23bp)
ATTCTGGTGTCCTAGGCGTAGAGGATGGAATTCTCGGGTGCCAAGGAACT14297960.4542827243974843RNA PCR Primer, Index 1 (100% over 25bp)
TGGTCCCGAGGGACGGAGGAGGCTAGGTTAGCCGATGGAATTCTCGGGTG12694430.4033344788398595No Hit
ACAGGGGAGCCGGCGACCGAAGCCCTGGAATTCTCGGGTGCCAAGGAACT10396410.3303205113703808RNA PCR Primer, Index 1 (100% over 25bp)
AAGCATAGAGACGGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC10024150.3184928599442887RNA PCR Primer, Index 6 (100% over 35bp)
GATGGATACTAGGCGCTGTGCGTTGGAATTCTCGGGTGCCAAGGAACTCC9590110.3047023000534033RNA PCR Primer, Index 1 (100% over 27bp)
CGCCGCGTGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAA9360510.29740732136262066No Hit
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC7822540.24854208452872278RNA PCR Primer, Index 1 (100% over 26bp)
AAACGATGGATACTAGGCGCTGTGCGTTGGAATTCTCGGGTGCCAAGGAA7807230.24805564670748637RNA PCR Primer, Index 1 (100% over 23bp)
CAGGGGAGCCGGCGACCGAAGCCCTGGAATTCTCGGGTGCCAAGGAACTC7339040.23318005405401285RNA PCR Primer, Index 1 (100% over 26bp)
CGATGGATACTAGGCGCTGTGCGTGGAATTCTCGGGTGCCAAGGAACTCC7217520.22931905313718398RNA PCR Primer, Index 1 (100% over 27bp)
GAAGGTCACGGCGAGACGAGCCGTTTATCTGGAATTCTCGGGTGCCAAGG6864700.21810906018560763Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGAGCCGGCGACCGAAGCCCTGGAATTCTCGGGTGCCAAGGAACTCC6518680.20711512060989073RNA PCR Primer, Index 1 (100% over 27bp)
CGCCCGTCACACTATGGGAGCTGGCCATGCCTGGAATTCTCGGGTGCCAA6494610.20635035520445894No Hit
TGGTCCCGAGGGACGGAGGAGGCTAGGTTAGCCGAATGGAATTCTCGGGT5711690.18147498622977454No Hit
ACACATGCAAGTCGGACGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG5706590.18131294619787997RNA PCR Primer, Index 1 (100% over 29bp)
TGGCGAGAGTCCAGTAGCCGAAAGCATCTGGAATTCTCGGGTGCCAAGGA5351060.17001684962151262RNA PCR Primer, Index 1 (100% over 22bp)
TGGTCCCGAGGGACGGAGGAGGCTAGGTTAGCCTGGAATTCTCGGGTGCC5244830.1666416510748147No Hit
CGCCCGTCACACTATGGGAGCTGGCCATGCCCTGGAATTCTCGGGTGCCA5006930.1590829601752625No Hit
TCTCATGGAGAGTTCGATCCTGGCTTGGAATTCTCGGGTGCCAAGGAACT5000290.15887199040824684RNA PCR Primer, Index 1 (100% over 25bp)
ACACATGCAAGTCGGACGGGAATGGAATTCTCGGGTGCCAAGGAACTCCA4982600.1583099339054596RNA PCR Primer, Index 1 (100% over 28bp)
AAAACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG4971540.15795852944413533RNA PCR Primer, Index 1 (100% over 29bp)
TGGTCCCGAGGGACGGAGGAGGCTAGGTTAGCCGTGGAATTCTCGGGTGC4827860.15339344870244695No Hit
ACGGCCCAGACTCCTACGGGAGGCAGCTGGAATTCTCGGGTGCCAAGGAA4785360.15204311510332563RNA PCR Primer, Index 1 (100% over 23bp)
ACGATGCCGACCAGGGATCAGCGGATGTTGGAATTCTCGGGTGCCAAGGA4711760.14970465503520017RNA PCR Primer, Index 1 (100% over 22bp)
ACACTATGGGAGCTGGCCATGCCTGGAATTCTCGGGTGCCAAGGAACTCC4476280.14222285371940968RNA PCR Primer, Index 1 (100% over 27bp)
AGGGGAGCCGGCGACCGAAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA4397800.13972934358154984RNA PCR Primer, Index 1 (100% over 28bp)
ACACTATGGGAGCTGGCCATGCCCTGGAATTCTCGGGTGCCAAGGAACTC4312190.13700929512459034RNA PCR Primer, Index 1 (100% over 26bp)
CTGCTAGGCGAAGCGGTGGAGTGCCGCACCTGGAATTCTCGGGTGCCAAG4124370.1310417737931322No Hit
TCAAACGAGGAAAGGCTTACGGTGGATACTGGAATTCTCGGGTGCCAAGG4095000.13010861384475117Illumina Small RNA Adapter 2 (100% over 21bp)
TATTCTGGTGTCCTAGGCGTAGAGGAATGGAATTCTCGGGTGCCAAGGAA3988430.1267226126292603RNA PCR Primer, Index 1 (100% over 23bp)
ACGGCCCAGACTCCTACGGGAGGCTGGAATTCTCGGGTGCCAAGGAACTC3987830.1267035490960962RNA PCR Primer, Index 1 (100% over 26bp)
TGGTTATCGGTTCAAGGACGCAAGGTGTCTGGAATTCTCGGGTGCCAAGG3987210.12668385011182667Illumina Small RNA Adapter 2 (100% over 21bp)
ACAGGGGAGCCGGCGACCGAAGCCTGGAATTCTCGGGTGCCAAGGAACTC3907880.12416333330198441RNA PCR Primer, Index 1 (100% over 26bp)
TATTCTGGTGTCCTAGGCGTAGAGGTGGAATTCTCGGGTGCCAAGGAACT3761900.11952517568316713RNA PCR Primer, Index 1 (100% over 25bp)
ATTCTGGTGTCCTAGGCGTAGAGGAATGGAATTCTCGGGTGCCAAGGAAC3700880.11758641436038161RNA PCR Primer, Index 1 (100% over 24bp)
TGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAA3565030.11327011272648431No Hit
CACACTATGGGAGCTGGCCATGCCCTGGAATTCTCGGGTGCCAAGGAACT3483230.11067111770511663RNA PCR Primer, Index 1 (100% over 25bp)
CAGGGGAGCCGGCGACCGAAGCCTGGAATTCTCGGGTGCCAAGGAACTCC3354790.10659025070579554RNA PCR Primer, Index 1 (100% over 27bp)
GAAGGTCACGGCGAGACGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTC3324030.10561292690558442RNA PCR Primer, Index 1 (100% over 26bp)
ACCCCGTAGGCTGAGGAGCAAAAGGAGGTGGAATTCTCGGGTGCCAAGGA3228180.10256752748262485RNA PCR Primer, Index 1 (100% over 22bp)
AGGGGAGCCGGCGACCGAAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG3222680.1023927784286209RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)