FastQCFastQC Report
Thu 28 Sept 2023
SRR2060718.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060718.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166221017
Total Bases8.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC17919821.078071854174734RNA PCR Primer, Index 12 (100% over 50bp)
GAAGGTCACGGCGAGACGAGCCGTTTATCTGGAATTCTCGGGTGCCAAGG14465810.8702756282618582Illumina Small RNA Adapter 2 (100% over 21bp)
CGATGCCGACCAGGGATCAGCGGATGTGGAATTCTCGGGTGCCAAGGAAC9994930.6013036245591014RNA PCR Primer, Index 1 (100% over 24bp)
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC8015220.48220256046201426RNA PCR Primer, Index 1 (100% over 26bp)
TGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAA6394740.38471308354466394No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCTGGAATTCTCGGGTGCCAAGGAACT4928400.29649680220642616RNA PCR Primer, Index 1 (100% over 25bp)
CCCTTCACGGCCGGCTTCTTAGAGGGATGGAATTCTCGGGTGCCAAGGAA4039550.24302281822761318RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGTGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAA3780750.22745318662079897No Hit
AAAGCGTCGTGCTGCTAGGCGTGGAATTCTCGGGTGCCAAGGAACTCCAG3426170.2061213474587272RNA PCR Primer, Index 1 (100% over 29bp)
TGCCACGATCCACTGAGATTCTGGAATTCTCGGGTGCCAAGGAACTCCAG3316300.19951147332951283RNA PCR Primer, Index 1 (100% over 29bp)
AAAGCGTCGTGCTGCTAGGCGATGGAATTCTCGGGTGCCAAGGAACTCCA3218030.19359946522286048RNA PCR Primer, Index 1 (100% over 28bp)
TAGCCCCGTAACTTCGGGAGAAGGGGTGCCTCCTGGAATTCTCGGGTGCC3212610.1932733933399048No Hit
TACGGTGGCGGCCTAAGCCCGGGCTTGGAATTCTCGGGTGCCAAGGAACT3035140.18259664480334636RNA PCR Primer, Index 1 (100% over 25bp)
AGGAAAAGCTCGAACGACCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT2988780.17980758714765896RNA PCR Primer, Index 1 (100% over 30bp)
ACACATGCAAGTCGGACGGGAATGGAATTCTCGGGTGCCAAGGAACTCCA2892670.17402552650727676RNA PCR Primer, Index 1 (100% over 28bp)
AAGCGTCGTGCTGCTAGGCGATGGAATTCTCGGGTGCCAAGGAACTCCAG2884080.1735087446853968RNA PCR Primer, Index 1 (100% over 29bp)
CGATGCCGACCAGGGATCAGCGGATTGGAATTCTCGGGTGCCAAGGAACT2832100.1703815829739509RNA PCR Primer, Index 1 (100% over 25bp)
TGCCGGCCGGGGGACGGACTGGGAATGGAATTCTCGGGTGCCAAGGAACT2639280.15878136517477812RNA PCR Primer, Index 1 (100% over 25bp)
AATGATTAACAGGGACAGTCGGGGGCTGGAATTCTCGGGTGCCAAGGAAC2612050.15714318484767784RNA PCR Primer, Index 1 (100% over 24bp)
AGATAAAAGGTCGACGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG2551260.1534860059242689RNA PCR Primer, Index 1 (100% over 29bp)
TACTCGGATAACCGTAGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAG2465920.1483518777893171No Hit
CGATGCCGACCAGGGATCAGCGGATGTTGGAATTCTCGGGTGCCAAGGAA2413310.1451868147335424RNA PCR Primer, Index 1 (100% over 23bp)
GAAGGTCACGGCGAGACGAGCCGTTTGGAATTCTCGGGTGCCAAGGAACT2359280.14193632325086783RNA PCR Primer, Index 1 (100% over 25bp)
GAAGGTCACGGCGAGACGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTC2325960.13993176326192253RNA PCR Primer, Index 1 (100% over 26bp)
ACGATGCCGACCAGGGATCAGCGGATGTGGAATTCTCGGGTGCCAAGGAA2279680.13714751847535622RNA PCR Primer, Index 1 (100% over 23bp)
TGGTCCCGAGGGACGGAGGAGGCTAGGTTAGCCGATGGAATTCTCGGGTG2227320.133997495635585No Hit
CCCTTCACGGCCGGCTTCTTAGAGGGACTGGAATTCTCGGGTGCCAAGGA2194670.13203324342552905RNA PCR Primer, Index 1 (100% over 22bp)
CGCTTGTGGAGACGTCGCTGCCGTTGGAATTCTCGGGTGCCAAGGAACTC2185980.1315104455172477RNA PCR Primer, Index 1 (100% over 26bp)
ACACATGCAAGTCGGACGGGATGGAATTCTCGGGTGCCAAGGAACTCCAG2181740.13125536345382846RNA PCR Primer, Index 1 (100% over 29bp)
CCCTTCACGGCCGGCTTCTTAGATGGAATTCTCGGGTGCCAAGGAACTCC2170310.13056772477814885RNA PCR Primer, Index 1 (100% over 27bp)
AAGCGTCGTGCTGCTAGGCGAAGCGGTGGTGGAATTCTCGGGTGCCAAGG2080070.12513880840952862Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGTCGTGCTGCTAGGCGAAGCGGTGGATGGAATTCTCGGGTGCCAAG2075470.12486206843506438No Hit
AAAACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAG2037220.12256091538653022RNA PCR Primer, Index 1 (100% over 29bp)
CAATGATTAGAGGCATTGGGGGCGCTGGAATTCTCGGGTGCCAAGGAACT2013320.12112307073659644RNA PCR Primer, Index 1 (100% over 25bp)
TACTCGGATAACCGTAGTAATTCTAGAGCTATGGAATTCTCGGGTGCCAA1968740.11844109941885386No Hit
ACGATGCCGACCAGGGATCAGCGGATGTTGGAATTCTCGGGTGCCAAGGA1929830.11610024020007048RNA PCR Primer, Index 1 (100% over 22bp)
AACCGTGAAAACGGGGTTGTGGGAGAGCTGGAATTCTCGGGTGCCAAGGA1831810.11020327230942162RNA PCR Primer, Index 1 (100% over 22bp)
ACGGTGGCGGCCTAAGCCCGGGCTTGGAATTCTCGGGTGCCAAGGAACTC1817730.10935620734410499RNA PCR Primer, Index 1 (100% over 26bp)
CGACCAGGGATCAGCGGATGTTGCTTGGAATTCTCGGGTGCCAAGGAACT1716700.10327815525277408RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)