FastQCFastQC Report
Thu 28 Sept 2023
SRR2060720.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2060720.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110170650
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC44482284.037579881756166RNA PCR Primer, Index 12 (100% over 50bp)
GAAGGTCACGGCGAGACGAGCCGTTTATCTGGAATTCTCGGGTGCCAAGG41238513.7431484701233946Illumina Small RNA Adapter 2 (100% over 21bp)
CGATGCCGACCAGGGATCAGCGGATGTGGAATTCTCGGGTGCCAAGGAAC5242800.4758799190165439RNA PCR Primer, Index 1 (100% over 24bp)
GCGACGGGGTATTGTAAGTGGCAGAGTGGCCTGGAATTCTCGGGTGCCAA5017100.4553935190543035No Hit
CCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTA4695970.42624510248419156RNA PCR Primer, Index 12 (100% over 47bp)
AATGATTAACAGGGACAGTCGGGGGCTGGAATTCTCGGGTGCCAAGGAAC4338270.39377729004957307RNA PCR Primer, Index 1 (100% over 24bp)
GCGACGGGGTATTGTAAGTGGCAGAGTGGCCTTGGAATTCTCGGGTGCCA3721800.3378213707552783No Hit
TACGGTGGCGGCCTAAGCCCGGGCTTGGAATTCTCGGGTGCCAAGGAACT3227110.29291921214951533RNA PCR Primer, Index 1 (100% over 25bp)
TGGCCGAGGGCACGTCTGCCTGGGTGTCACATGGAATTCTCGGGTGCCAA2764610.25093888435803907No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCCTGGAATTCTCGGGTGCCAA2691210.24427649287718645No Hit
TTACGGTGGCGGCCTAAGCCCGGGCTTGGAATTCTCGGGTGCCAAGGAAC2544710.23097894039837288RNA PCR Primer, Index 1 (100% over 24bp)
ATGATTAACAGGGACAGTCGGGGGCTGGAATTCTCGGGTGCCAAGGAACT2544680.23097621735008372RNA PCR Primer, Index 1 (100% over 25bp)
GTCCTGCGGAAAAATAGCTCGACGCCAGGATGTGGAATTCTCGGGTGCCA2369530.21507815375510628No Hit
CGCCCGCGACGTCGCGAGAAGTCCACTAAACCTTGGAATTCTCGGGTGCC2231300.20253125492134247No Hit
CGCCCGCGACGTCGCGAGAAGTCCACTAAACCTGGAATTCTCGGGTGCCA2099190.19053985793857076No Hit
GAAGGTCACGGCGAGACGAGCCGTTATCTGGAATTCTCGGGTGCCAAGGA2051350.18619750360009674RNA PCR Primer, Index 1 (100% over 22bp)
TGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAAGGAACTC1993400.18093748198817017RNA PCR Primer, Index 1 (100% over 26bp)
ACGATGCCGACCAGGGATCAGCGGATGTGGAATTCTCGGGTGCCAAGGAA1927050.17491500685527406RNA PCR Primer, Index 1 (100% over 23bp)
GAAGGTCACGGCGAGACGAGCCGTTTATGGAATTCTCGGGTGCCAAGGAA1911050.17346271443437977RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGTGCCGGCCGGGGGACGGACTGGGATGGAATTCTCGGGTGCCAA1867310.16949251002875992No Hit
CTCGATGGTTCACGGGATTCTGCAATTCTGGAATTCTCGGGTGCCAAGGA1835870.16663875542170262RNA PCR Primer, Index 1 (100% over 22bp)
AATGATTAACAGGGACAGTCGGGGGCATGGAATTCTCGGGTGCCAAGGAA1745810.15846416445759376RNA PCR Primer, Index 1 (100% over 23bp)
GCACCAGTGGTCTAGTGGTAGAATAGTACCCTTGGAATTCTCGGGTGCCA1725030.15657799967595726No Hit
ACGGTGGCGGCCTAAGCCCGGGCTTGGAATTCTCGGGTGCCAAGGAACTC1717420.1558872530932694RNA PCR Primer, Index 1 (100% over 26bp)
CGATGCCGACCAGGGATCAGCGGATTGGAATTCTCGGGTGCCAAGGAACT1684940.15293909947885392RNA PCR Primer, Index 1 (100% over 25bp)
GCCCGCGACGTCGCGAGAAGTCCACTAAACCTGGAATTCTCGGGTGCCAA1672510.15181084980437168No Hit
GAAGGTCACGGCGAGACGAGCCGTTTGGAATTCTCGGGTGCCAAGGAACT1592960.14459023342423777RNA PCR Primer, Index 1 (100% over 25bp)
TCGATGGTTCACGGGATTCTGCAATTCTGGAATTCTCGGGTGCCAAGGAA1505690.13666888595102233RNA PCR Primer, Index 1 (100% over 23bp)
GAAGGTCACGGCGAGACGAGCCGTTGGAATTCTCGGGTGCCAAGGAACTC1474840.13386868462698548RNA PCR Primer, Index 1 (100% over 26bp)
AGCGCGCGACCTATACCCGGCCGTCGGGGCTGGAATTCTCGGGTGCCAAG1450800.1316866152645918No Hit
GCCCGCGACGTCGCGAGAAGTCCACTAAACCTTGGAATTCTCGGGTGCCA1442140.13090056199178274No Hit
AACGATGCCGACCAGGGATCAGCGGATGTTGGAATTCTCGGGTGCCAAGG1396000.12671251372302877Illumina Small RNA Adapter 2 (100% over 21bp)
ACGATGCCGACCAGGGATCAGCGGATGTTGGAATTCTCGGGTGCCAAGGA1377720.12505326963215702RNA PCR Primer, Index 1 (100% over 22bp)
GAAGGTCACGGCGAGACGAGCCGTTTATCTGGAATTCTCGTATGCCGTCT1361280.12356103916968811No Hit
GCACCAGTGGTCTAGTGGTAGAATAGTACCTGGAATTCTCGGGTGCCAAG1328130.12055207081014771No Hit
AGCGGAGGAAAAGAAACTAACAAGGATTCCCTTGGAATTCTCGGGTGCCA1314100.11927859189357602No Hit
ATGGTTCACGGGATTCTGCAATTCTGGAATTCTCGGGTGCCAAGGAACTC1305840.11852884593128933RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGCGGAAAAATAGCTCGACGCCAGGATGTGGAATTCTCGGGTGCCAA1303180.11828740231631565No Hit
TGGTCGGCTTGTCCCTTCGGTCGGCTGGAATTCTCGGGTGCCAAGGAACT1256530.11405306222664566RNA PCR Primer, Index 1 (100% over 25bp)
GAAGGTCACGGCGAGACGAGCCGTTTATTGGAATTCTCGGGTGCCAAGGA1200830.10899726923640735RNA PCR Primer, Index 1 (100% over 22bp)
AGCTCGCGTTGACTACGTCCCTGCCCTTGGAATTCTCGGGTGCCAAGGAA1178710.10698947496452095RNA PCR Primer, Index 1 (100% over 23bp)
GATGGTTCACGGGATTCTGCAATTCTGGAATTCTCGGGTGCCAAGGAACT1174710.10662640185929737RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)