FastQCFastQC Report
Sat 23 Sept 2023
SRR20613391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20613391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13592783
Total Bases406.3 Mbp
Sequences flagged as poor quality0
Sequence length13-48
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGG4040792.9727466406253966No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2038971.5000386602213838No Hit
GACTGCATAATTTGTGGTAGTGGGGG1723111.2676653485897627No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1285310.945582666919644No Hit
GACTGCATAATTTGTGGTAGTGGGGGA739780.5442446922017368No Hit
AATGGATTTTTGGAGCAGGGAGATGGA731150.5378957348175131No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAAC661930.48697165253061125No Hit
AAATGGATTTTTGGAGCAGGGAGATGGA639920.4707792363050304No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA431710.3176023629598148No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA363200.26720061668018974No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG309430.2276428601854381No Hit
TGACTGCATAATTTGTGGTAGTGGGGG278820.2051235571111523No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGA263550.19388965453211457No Hit
CTCGACTGCATAATTTGTGGTAGTTGGGG255310.1878276141096345No Hit
ACTGCATAATTTGTGGTAGTGGGGG248870.18308980581827872No Hit
ATGGATTTTTGGAGCAGGGAGATGGA240360.17682913057612998No Hit
GACTGCATAATTTGTGGTAGTGGGG190700.14029503744744545No Hit
AATGGATTTTTGGAAATAGGAGATGGA158460.11657656860997487No Hit
AAATGGATTTTTGGAAATAGGAGATGGA157080.1155613239761129No Hit
GGAGATACCATGATCACGAAGGTGGT146240.10758650380867554No Hit
CACCTCTAGCATCACCAGTATTAGAGGCA145440.10699795619484251No Hit
TCGACTGCATAATTTGTGGTAGTGGGG137000.1007887788689042No Hit

[OK]Adapter Content

Adapter graph