FastQCFastQC Report
Sat 23 Sept 2023
SRR20613392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20613392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18511253
Total Bases553.7 Mbp
Sequences flagged as poor quality0
Sequence length13-48
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGG2749191.4851452789284443No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG2184211.1799363338613544No Hit
GACTGCATAATTTGTGGTAGTGGGGG1800600.9727056293812202No Hit
CGACTGCATAATTTGTGGTAGTGGGGG1141200.616489872403559No Hit
AATGGATTTTTGGAGCAGGGAGATGGA1116620.6032114627788837No Hit
AAATGGATTTTTGGAGCAGGGAGATGGA791930.4278100461378816No Hit
GACTGCATAATTTGTGGTAGTGGGGGA754650.40767094480314214No Hit
ATGGATTTTTGGAGCAGGGAGATGGA320890.17334861124743958No Hit
TAAATGGATTTTTGGAGCAGGGAGATGGA267550.14453370606517021No Hit
TGACTGCATAATTTGTGGTAGTGGGGG258070.13941249682017745No Hit
ACTGCATAATTTGTGGTAGTGGGGG249510.1347882825652051No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAAC247490.1336970544349429No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA228500.12343842958658713No Hit
GACTGCATAATTTGTGGTAGTGGGG208610.11269361398712448No Hit
CTCGACTGCATAATTTGTGGTAGTGGGG203870.11013300936462811No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA199070.10753999202539127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)