FastQCFastQC Report
Sat 23 Sept 2023
SRR20620076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20620076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6065336
Total Bases909.8 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA3488625.751734116626021No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA2920394.814885770549233No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA2073603.418771853694503No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA1359842.2419862642399364No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1281232.1123809134399147No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA701871.1571823885766592No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA610491.0065229692139066No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA588010.9694598947197649No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA511220.842855202086084No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA491070.8096336295301696No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA415120.6844138560501841No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA356450.587683848017653No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA351380.5793248716971326No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA349990.577033160240422No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA338170.5575453692919898No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA312620.5154207450337458No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA304350.5017858862229562No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA298700.49247065620107444No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAA285770.4711527935138301No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA281970.46488768305663525No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAACAAAAAA260970.43026470421424307No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA202060.33313900499494176No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA190590.31422826369388274No Hit
GGGGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAAAA188100.31012296763114194No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA180390.2974113882561494No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAACAAAAAAA176720.2913606105251218No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA167480.2761264998344692No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA158350.2610737476044196No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA153740.2534731793918754No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA151050.24903814067349278No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAAA150080.24743888879362988No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAACAAAA139690.23030875783303675No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAAA137480.22666510148819458No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAACAAAAAA136780.22551100219344813No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGAAAAAAAAAACAAA130400.21499221147847375No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA125700.2072432590708907No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA123630.20383042258499776No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA122740.20236306776739163No Hit
GGGGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT117970.19449870543033393Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA115730.19080558768714545No Hit
GGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAACAAAAAAAAA113730.18750816113072713No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA113570.18724436700621366No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAACAAAAAAA113290.1867827272883151No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA111380.18363368492693563No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAAA110570.18229822717158622No Hit
GGGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAACAAAAAAAA108100.1782259053744096No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC104820.17281812582188358No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAACAAAAAAAA103000.1698174676555429No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAGAAAAAAAAAACAA99350.1637996641900795No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA97730.16112874867938068No Hit
CCCCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGATC97630.16096387735155976No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA95620.15764996366235937No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAAACAAAAAAAAA94060.15507797094835307No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCGAAAAAAAAAACAAAA93220.1536930517946574No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAA93010.15334682200623345No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA88640.14614194498045943No Hit
CCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA88190.14540002400526533No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA87470.14421295044495475No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA86950.14335561954028597No Hit
GGGGGCCCGTCTCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA86520.14264667283065605No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTAAAAAAAAAACAAAAAAAA83870.1382775826434018No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA83730.13804676278445252No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAAA77770.12822043164632593No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA77530.12782474045955575No Hit
GGGGGAATAATGGAATAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA76120.12550005473728085No Hit
GGGGGGCCGATCGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA74080.12213667964973415No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAACAAAAAAA72420.11939981560790697No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAGAAAAAAAA71440.117784076595262No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAACAAAAAA70800.11672890009720813No Hit
GGGGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAA70290.11588805632532147No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA69920.11527803241238407No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAAACAAAAA69850.11516262248290944No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAGAAAAAAAAAAC66010.1088315634945863No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA63990.10550116267260379No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAGAAAAAAA63390.1045119347056783No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAACAAAAAA62620.10324242548145725No Hit
GGGGGGGTCGGCGGCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA62620.10324242548145725No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAGAAAAAAAAA62210.10256645303739151No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAAA61700.10172560926550483No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA61670.10167614786715856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)