FastQCFastQC Report
Sat 23 Sept 2023
SRR20620077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20620077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6305009
Total Bases945.7 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA2914064.6218173518864125No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA2661174.22072355487518No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA1547332.454128138437233No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA1488942.361519230186666No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA1018441.6152871470920978No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA752761.1939078913289418No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAA639371.0140667523234304No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAA552640.8765094546256794No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAA513570.8145428499784854No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAACAAAAA446670.7084367365692895No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAACAAA397760.6308634928197564No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA392980.6232822189468723No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAACAAAAA344050.5456772543861556No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAAA329750.52299687438987No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCAAAAAAAAAACAAAAAA327960.5201578617889364No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCATAAAAAAAAAACAAAAAA324700.514987369566007No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAAA320030.5075805601546326No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAACAAAA289830.45968213526737234No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAAA274760.4357805040405176No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAAAAAAAAA249960.39644669817283373No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAA239320.3795712266231499No Hit
GGGGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAAAAAAAAAACAAAA213450.33854035735714255No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAACAAAA211850.3360026924624533No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAACAAAAAA192040.3045832289850815No Hit
CCCCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAA191740.3041074168173273No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAACAAAA189310.30025333825851797No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAAACAAAAAA184690.2929258308751026No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAACAAAAAA179300.28437707226111814No Hit
GGGGGAATAATGGAATAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA177470.28147461803781726No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAACAAAAA171500.2720059558995078No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAACAAAAAA157110.24918283225289609No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAACAAAAAAA156920.24888148454665174No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAAA150670.23896873105177172No Hit
GGGGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACACGTCT140710.2231717670823309Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGAT136270.21612974699956813No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGAAAAAAAAAACAAAAAAA133620.21192673951773897No Hit
GGGCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAACAAAAA132750.21054688423125167No Hit
CCCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAA118080.1872796692280693No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAA117160.18582051191362298No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGTTAAAAAAAAAACAAAAAA117110.18574120988566392No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAACAA117040.18563018704652126No Hit
GGGCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAA116850.1853288393402769No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGAAAAAAAAAACAAA116140.18420275054325855No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAA115050.18247396633375146No Hit
GGGGGGGCTCTCGCTTCTGGCGCCAAGCGTAAAAAAAAAACAAAAAAAAA110210.17479753002731638No Hit
CCCCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAACAAAA110010.1744803219154802No Hit
CCCCCGCCTGGATACCGCAGCTAGGAAAAAAAAAACAAAAAAAAAAGATC108010.1713082407971186No Hit
GGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAACAAAAAAAA107560.17059452254548724No Hit
GGGGATCGAGGCCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACAC101170.16045972337232192No Hit
GGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAACAAAAAAAAA101000.16019009647726118No Hit
GGGGGGCCGATCGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA100110.15877852037959025No Hit
GGGCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAACAAAAAAA96690.15335426166719193No Hit
GGGGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAA96360.15283086828266224No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCTAAAAAAAAAACAAAAAA95310.1511655256955224No Hit
CCCCCGCCTGGATACCGCAGCTAGGAATAATGGAACAAAAAAAAAACAAA94440.1497856704090351No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGAAAAAAAAACAAAAAAA94290.14954776432515798No Hit
GGGCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAACAAA93570.1484058151225478No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACATAAAAAAAAAACAAA90070.142854673165415No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAACAAAAAA89060.1412527722006424No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCGAAAAAAAAAACAAAA86650.13743041445301665No Hit
GGGGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAACAAAAAAAAAA84640.13424247292906322No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAGAAAAAAAAAACAA84330.13375080035571718No Hit
GGGGACGTACGCAGTTTTATCCGGTAAAGCAAAAAAAAAACAAAAAAAAA83750.1328308968313923No Hit
GGGGGCCCGTCTCGAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCACA82310.13054699842617196No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAGAAAAAAAA78890.1251227397137736No Hit
CCCCCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAACAAAAAAAA76600.12149070683324956No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAGAAAAAAAAAACAA76210.12087215101516904No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAAAAAAAAAAAAA75690.12004740992439503No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAACAAAAAA75650.1199839683020278No Hit
CCCCGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAA75100.11911164599447835No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTAAAAAAAAAACAAAAAAAA74630.11836620693166339No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAA72670.11525756743566902No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAGAAAAAAAAA72240.11457556999522125No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAGAAAAAAA71940.11409975782746702No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAGAAAAAAAAAAC71580.11352878322616192No Hit
GGGGGGCCGCCGGTGAAATACCACTACTCTCAAAAAAAAAAACAAAAAAA70110.11119730360416615No Hit
CCCCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAACAAAAAA68700.10896098641572122No Hit
GGGGGGCCGATCGCACAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCA68470.10859619708710963No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAGAAAAAA66920.1061378342203794No Hit
GGGGGCCCGTCTCGCAAAAAAAAAACAAAAAAAAAAGATCGGAAGAGCAC66290.1051386286680955No Hit
CCCCGCTGCGATCTATTGAAAGTCAGCCCTCGACACGAAAAAAAAAACAA63570.1008245983471237No Hit
CCCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAGAAAAAAAAAACA63360.10049152982969574No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)