FastQCFastQC Report
Tue 26 Sept 2023
SRR2064017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2064017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134204905
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT7747800.5773112391085855No Hit
CTGTAGGCACCATCAATTCGTNTGCCGTCTTCTGCTTGAAAAAAAAAAAA4958830.36949692710560766Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTTTTTCACTGACCCGGTGNGGCGGGGCTGTAGGCACCATCAATTCGT3400880.253409515844447No Hit
CCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT2885370.21499735795796732No Hit
CCGCCGAGGGCGCACCACCGGNCCGTCTCGCCTGTAGGCACCATCAATTC2810820.20944241941082556No Hit
CGTGGGGGGCCCAAGTCCTTCNGATCGAGGCCCTGTAGGCACCATCAATT2266300.1688686415746131No Hit
GTGGGGGGCCCAAGTCCTTCTNATCGAGGCCCTGTAGGCACCATCAATTC2161200.16103733317347826No Hit
CCGCCGAGGGCGCACCACCGGNCCGTCTCGCTGTAGGCACCATCAATTCG1654220.12326077053592044No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)