FastQCFastQC Report
Mon 25 Sept 2023
SRR2064018.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2064018.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100619445
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT15400261.5305451148135432No Hit
CCGCCGAGGGCGCACCACCGGNCCGTCTCGCCTGTAGGCACCATCAATTC9270400.9213328497289962No Hit
CGTGGGGGGCCCAAGTCCTTCNGATCGAGGCCCTGTAGGCACCATCAATT8273640.8222704865843774No Hit
CGTTTTTTCACTGACCCGGTGNGGCGGGGCTGTAGGCACCATCAATTCGT8190760.8140335101232172No Hit
GTGGGGGGCCCAAGTCCTTCTNATCGAGGCCCTGTAGGCACCATCAATTC7807260.7759196048040217No Hit
CCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT6348590.6309506080062358No Hit
CTCCCGGGGCTACGCCTGTCTNAGCGTCGCTCTGTAGGCACCATCAATTC4370750.43438422861505543No Hit
CTGTAGGCACCATCAATTCGTNTGCCGTCTTCTGCTTGAAAAAAAAAAAA3327890.33074024608265334Illumina Paired End PCR Primer 2 (95% over 21bp)
TCCGCCGAGGGCGCACCACCGNCCCGTCTCGCCTGTAGGCACCATCAATT3114480.3095306280013769No Hit
TCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTTCTGTAGGCACCATCAAT2843900.28263920557303807No Hit
TCGTTTTTTCACTGACCCGGTNAGGCGGGGCTGTAGGCACCATCAATTCG2683900.2667377066132694No Hit
GCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT2680860.2664355781330338No Hit
TGGATGGTTTAGTGAGGCCCTNGGATCGGCCCTGTAGGCACCATCAATTC2223550.22098611257496004No Hit
CCGCCGAGGGCGCACCACCGGNCCGTCTCGCTGTAGGCACCATCAATTCG2187510.21740429993427215No Hit
TGTGGGGGGCCCAAGTCCTTCNGATCGAGGCCCTGTAGGCACCATCAATT2128070.21149689307071812No Hit
GGATGGTTTAGTGAGGCCCTCNGATCGGCCCTGTAGGCACCATCAATTCG1389830.1381273768703455No Hit
TGGGGGGCCCAAGTCCTTCTGNTCGAGGCCCTGTAGGCACCATCAATTCG1312250.13041713756222767No Hit
CCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTTCTGTAGGCACCATCAAT1183780.1176492277412184No Hit
TTGGGGGGCCCAAGTCCTTCTNATCGAGGCCCTGTAGGCACCATCAATTC1143030.11359931472490234No Hit
TCACCTCTAGCATCACCAGTANTAGAGGCCTGTAGGCACCATCAATTCGT1100660.1093883990316186No Hit
ACCTCTAGCATCACCAGTATTNGAGGCCTGTAGGCACCATCAATTCGTAT1045850.10394114179421285No Hit
AGGTCGGTTTCTATCTACTTCNAATTCCTCTGTAGGCACCATCAATTCGT1031160.10248118542096908No Hit
GGGGCTCTCGCTTCTGGCGCCNAGCGCCCGGCTGTAGGCACCATCAATTC1010730.10045076277254363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)