FastQCFastQC Report
Mon 25 Sept 2023
SRR2064019.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2064019.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81871314
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT9984941.219589562224444No Hit
CCGCCGAGGGCGCACCACCGGNCCGTCTCGCCTGTAGGCACCATCAATTC5639940.6888786467992928No Hit
CGTTTTTTCACTGACCCGGTGNGGCGGGGCTGTAGGCACCATCAATTCGT5471870.6683500890189695No Hit
TCGTTTTTTCACTGACCCGGTNAGGCGGGGCTGTAGGCACCATCAATTCG4471230.5461290141257535No Hit
TAGTCACTTAGCGATGTACACNGACTGTGCTGTAGGCACCATCAATTCGT3579900.43725938000701936No Hit
TCCGCCGAGGGCGCACCACCGNCCCGTCTCGCCTGTAGGCACCATCAATT3223320.39370566350016073No Hit
CCGCCGAGGGCGCACCACCGGNCCGTCTCGCTGTAGGCACCATCAATTCG3011840.3678748822817232No Hit
ACCTCTAGCATCACCAGTATTNGAGGCCTGTAGGCACCATCAATTCGTAT2713200.33139812559988957No Hit
AGTCCGCCCGGAGGATTCAACNCGGCGGCGGGCTGTAGGCACCATCAATT2623660.32046145002631815No Hit
CTGTAGGCACCATCAATTCGTNTGCCGTCTTCTGCTTGAAAAAAAAAAAA2167830.26478505035353406Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT2034530.24850340132564624No Hit
GTGGGGGGCCCAAGTCCTTCTNATCGAGGCCCTGTAGGCACCATCAATTC1946080.23769986151681893No Hit
CTAAACCATTCGTAGACGACCNGCTTCTGTAGGCACCATCAATTCGTATG1856170.2267179930689765No Hit
AGTCACTTAGCGATGTACACTNACTGTGCTGTAGGCACCATCAATTCGTA1836350.22429712072289448No Hit
TCCGCCGAGGGCGCACCACCGNCCCGTCTCGCTGTAGGCACCATCAATTC1753620.21419223832171547No Hit
AGCGAGCGCACGGGGTCGGCGNCGACCTGTAGGCACCATCAATTCGTATG1723930.21056581551872977No Hit
TGGATGGTTTAGTGAGGCCCTNGGATCGGCCCTGTAGGCACCATCAATTC1456070.17784861740462599No Hit
TTGGGGGGCCCAAGTCCTTCTNATCGAGGCCCTGTAGGCACCATCAATTC1416060.17296167983819094No Hit
TCTAAACCATTCGTAGACGACNTGCTTCTGTAGGCACCATCAATTCGTAT1410840.17232409388226014No Hit
GCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT1184980.1447368977124271No Hit
TTCCCGGGGCTACGCCTGTCTNAGCGTCGCTCTGTAGGCACCATCAATTC1045740.12772971983813525No Hit
TCGACTGCATAATTTGTGGTANTGGGGCTGTAGGCACCATCAATTCGTAT1038060.12679166233975434No Hit
TCGCCGAGGGCGCACCACCGGNCCGTCTCGCCTGTAGGCACCATCAATTC987130.12057092426780887No Hit
TCGACTGCATAATTTGTGGTANTGGGGGCTGTAGGCACCATCAATTCGTA982440.11999807405069865No Hit
TCCCGGGGCTACGCCTGTCTGNGCGTCGCTCTGTAGGCACCATCAATTCG935700.11428911474414592No Hit
TGGGGGGCCCAAGTCCTTCTGNTCGAGGCCCTGTAGGCACCATCAATTCG874240.10678221189902974No Hit
TGTGGGGGGCCCAAGTCCTTCNGATCGAGGCCCTGTAGGCACCATCAATT852200.10409018230732195No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)