FastQCFastQC Report
Mon 25 Sept 2023
SRR2064020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2064020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92417958
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT16284751.7620763704820224No Hit
CGTGGGGGGCCCAAGTCCTTCNGATCGAGGCCCTGTAGGCACCATCAATT6994520.7568355924938311No Hit
CCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT6562840.7101260558040029No Hit
GTGGGGGGCCCAAGTCCTTCTNATCGAGGCCCTGTAGGCACCATCAATTC5487390.593757979374528No Hit
TCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTTCTGTAGGCACCATCAAT3162510.3421964809047177No Hit
GCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTCTGTAGGCACCATCAATT3019070.32667568785711537No Hit
TGTGGGGGGCCCAAGTCCTTCNGATCGAGGCCCTGTAGGCACCATCAATT2950400.3192453137733253No Hit
CCGCCGAGGGCGCACCACCGGNCCGTCTCGCCTGTAGGCACCATCAATTC2789800.3018677387353657No Hit
CTGTAGGCACCATCAATTCGTNTGCCGTCTTCTGCTTGAAAAAAAAAAAA2549950.27591499046105306Illumina Paired End PCR Primer 2 (95% over 21bp)
TGGATGGTTTAGTGAGGCCCTNGGATCGGCCCTGTAGGCACCATCAATTC2296470.24848742059416631No Hit
CTCCCGGGGCTACGCCTGTCTNAGCGTCGCTCTGTAGGCACCATCAATTC1713900.18545097047047934No Hit
TCCGCCGAGGGCGCACCACCGNCCCGTCTCGCCTGTAGGCACCATCAATT1663200.17996502368078723No Hit
TAGTCACTTAGCGATGTACACNGACTGTGCTGTAGGCACCATCAATTCGT1400200.15150735098475127No Hit
TGCCGCCTGGATACCGCAGCTNGGAATAATGGAACTGTAGGCACCATCAA1389500.15034956734274524No Hit
CGATCAAAAGGGACAAGCATCNAGCACGCAACCTGTAGGCACCATCAATT1308190.14155149370428635No Hit
CCTCCCGGGGCTACGCCTGTCNGAGCGTCGCTTCTGTAGGCACCATCAAT1283530.13888318112373788No Hit
TTTGGTGACTCTAGATAACCTNGGGCCGATCGCACCTGTAGGCACCATCA1138540.12319467175416275No Hit
TCGTGGGGGGCCCAAGTCCTTNTGATCGAGGCCCTGTAGGCACCATCAAT1098880.118903297993232No Hit
TCGACTGCATAATTTGTGGTANTGGGGGCTGTAGGCACCATCAATTCGTA1097590.11876371473171912No Hit
TTGGGGGGCCCAAGTCCTTCTNATCGAGGCCCTGTAGGCACCATCAATTC1091360.11808960332146702No Hit
TCGTTTTTTCACTGACCCGGTNAGGCGGGGCTGTAGGCACCATCAATTCG1079370.11679223641794811No Hit
CGTGGGGGGCCCAAGTCCTTCNGATCGAGGCCTGTAGGCACCATCAATTC1012680.1095761064099685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)