FastQCFastQC Report
Mon 25 Sept 2023
SRR2064021.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2064021.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42166791
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT27700256.569209878930554No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT11335582.6882719152140364No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC9632472.2843735014125217No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT9554182.2658067577397576No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC8850632.0989574473428627No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC5685761.348397605120105No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4986591.1825870268382528No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT4836261.1469357485609943No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT4490011.0648213661788966No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT4301941.020219916663803No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG3070940.7282840185775579No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC3042070.7214373984494101No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG2605790.6179720908807123No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1765150.4186114138967796No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT1665730.39503361780601237No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC1665090.39488183959742157No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA1646530.39048027154829024No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT1468990.34837604787141613No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1423620.3376163958030385No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG1361370.32285359348308007No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC1338950.3175366131133858No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG1319800.3129951245282099No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1319600.3129476938380253No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATTCGT1293250.30669870040620356No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG1259870.29878251821439294No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG1188850.2819398801298396No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCTGTAGGCACCATCAATTCGT1168760.277175467300796No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1127540.2674000020537489No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT1085040.2573209803895203No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1061080.25163878370540455No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1050140.24904432495230663Illumina Single End Adapter 1 (95% over 22bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG1044830.2477850401279054No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATTC987090.23409179987160986No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTCGTA954230.22629893747427923No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATTCG879240.2085148001895615No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTACTGTAGGCACCATCAATTC857720.20341125792569797No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC835540.19815119438422527No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT806770.1913282896011698No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATTCG792700.18799154054668282No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT786220.1864547861847016No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAACCTGTAGGCACCATCAATT777840.18446744026596665No Hit
TTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATTCGT744310.17651568505651757No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA743910.17642082367614836No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC716490.169918076051839No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATT712130.16888408700581461No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATTCG683390.16206829682628685No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCACTGTAGGCACCATCAATTCG676320.16039162192826104No Hit
GCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT652840.15482325890058837No Hit
TCGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTC647500.1535568594726594No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTGTAGGCACCATCAATTCGTA645800.1531536986060903No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATTC636890.1510406613583661No Hit
CTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCGTA623830.1479434372893114No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATTCGTA604900.14345412246333852No Hit
GGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT596320.14141934585441893No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC583430.13836243787202113No Hit
ACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAATTCG547560.12985574358741217No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTCGTATGCCGT538950.12781385237496495No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTCG531820.12612294826988374No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCCTGTAGGCACCATCAATTCG522440.12389844890022578No Hit
GGAGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATTCGTA519170.12312295711570746No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT514830.12209371113870153No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATT495970.11762099705429328No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATTCGTA493670.1170755441171703No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATTC487820.1156881964292706No Hit
TTGTCAAAGTGAAGAAATTCAATGAAGCGCCTGTAGGCACCATCAATTCG486440.11536092466699684No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATTCGT485330.11509768433647226No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATT479850.11379808342541409No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTACTGTAGGCACCATCAATTC477830.11331903345454958No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGGCTGTAGGCACCATCAATTCG476760.11306527926206193No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT474590.11255065627355898No Hit
TGGCGGAGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATT467100.11077437692614552No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTCG465190.11032141383488252No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATT450210.10676885514005559No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGCTGTAGGCACCATCAATT443710.10522735770905592No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCCTGTAGGCACCATCAATTCGT443170.10509929484555748No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTC442740.10499731886166058No Hit
TGGCGGAGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTC442520.10494514510245752No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATT437690.10379969393449931No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATTCG425900.10100365474811682No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATT425810.10098231093753375No Hit
TTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATT424000.10055306319136308No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT421730.1000147248577678No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)