FastQCFastQC Report
Mon 25 Sept 2023
SRR2064022.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2064022.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40831102
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT15801623.8699959653305465No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT12383023.0327420504104934No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT7730331.8932454970233232No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC7329011.7949576771158418No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT7194931.7621199643350307No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC6483141.5877945199715648No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG5547761.3587093485745254No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT4211161.0313608484042385No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT3502890.8578974919658059No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT3450680.8451106707822875No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC3178830.7785315223674345No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT2720990.6664013133909538No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTCG2301790.5637344786824514No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC2007490.49165707063208824No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1833310.4489984130234839No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG1680020.411455953356341No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTCGTATG1364510.33418397573496794No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA1341950.3286587758518004No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTC1158650.28376652680106457No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGTAT1093430.26779340905371596No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1045280.2560009279200939No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1035950.2537159050960711No Hit
CCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT1033510.253118321420764No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC938800.22992276818783874No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC888420.21758413476080074No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG878430.21513747045083428No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT876870.21475540875678542No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGTA873300.2138810752646353No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAACCTGTAGGCACCATCAATT866680.2122597621783512No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA850330.20825546173110882Illumina Single End Adapter 1 (95% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATT812300.19894148338195722No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG746060.1827185560654229No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCCTCAATTCGT745670.18262304064191068No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCTGTAGGCACCATCAATTCGT742120.18175360537660729No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT720340.1764194363404642No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATTCGT717340.17568470231344724No Hit
TGTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATTC712890.17459484684003876No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT670820.16429142666783766No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATTCGTAT657300.1609802253194146No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATTCGTA647850.1586658131343112No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCTGTAGGCACCATCAATTCGT636180.15580769776921524No Hit
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATTCGTAT596470.14608226836493415No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGGACCATCAATTCGT585470.14338824359920532No Hit
GACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTATG568360.13919781053178532No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG546270.13378771897951713No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG540860.13246274861746324No Hit
GGAGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATTCGTA536800.13146840856756695No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTCG529990.12980056232623846No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATT522000.12784372070094996No Hit
GCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT504620.12358716157109843No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATTCGTA492080.12051597333816756No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATTCG491400.12034943362537705No Hit
TGCTGAGAAGACGGTCGAACTTGACTATCTAGCTGTAGGCACCATCAATT490230.12006288735484044No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATTC483310.11836810086585466No Hit
GGAGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTCGTAT477040.11683250674938922No Hit
ATGTACACGGAGTCGAGCACCCGCAACGCGACTGCTGTAGGCACCATCAA468790.11481198817509261No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG465460.11399643340510379No Hit
TGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATT453510.11106974286415293No Hit
TGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTAT449040.10997498916389765No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTACTGTAGGCACCATCAATTC446460.10934311790066309No Hit
CCAACTTCTTAGAGGGACAAGTGGCGTTCTGTAGGCACCATCAATTCGTA442510.10837571809842408No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC435130.10656827239196237No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATTCGT433450.10615682133683288No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC427360.10466531126198848No Hit
TAGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGT408750.10010751118105998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)