FastQCFastQC Report
Mon 25 Sept 2023
SRR2064023.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2064023.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77756301
Total Bases3.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC42591955.477620392461827No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT33590824.320012599364777No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTC29724163.8227332856278746No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT28705283.6916982457794645No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATTC27300633.5110505063763258No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT22067592.8380452408609305No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCG7165620.9215484671782419No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATTCG6019020.7740877488500899No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATT5806630.746772920692305No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5462560.7025231305691869No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4727900.6080407554366559No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATT3996000.5139133354607494No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTC3909750.5028209868162324No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT3868120.49746707987047895No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTC3831780.49279350364158914No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATTCGT3609900.4642581956155553No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATT3478620.4473746764265445No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATTCG3162740.4067503159647473No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAACTGTAGGCACCATCAAT3110380.4000164565441455No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATTCG2978450.38304934284361086No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2903110.3733600959232873No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT2810930.36150510811979086No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTCG2594200.3336321258389079No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGCTGTAGGCACCA2419090.3111117644343704No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2250180.2893887660628301No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC2205740.2836734736134117No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGCTGTAGGCACCATCAATTCGTA2131530.274129552536199No Hit
CTCCCGGGGCTACGCCTGTCTGAGGGTCGCTCTGTAGGCACCATCAATTC2066640.26578424814729806No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTAT2011710.2587198688888248No Hit
CGATCAAAAGGGACAAGCATCAAGCACGCAACCTGTAGGCACCATCAATT1855540.23863532294315287No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATTCG1671690.214990936875971No Hit
GGCGGAGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATTC1661180.2136392779281No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1514890.19482536855759122No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATTCG1512700.1945437193572261No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1508240.19397013240123137No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1506720.19377464985120627No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1458400.18756036247146066Illumina Single End Adapter 1 (95% over 22bp)
GTGGGGGGCCCAAGTCCTTCTGATGGAGGCCCTGTAGGCACCATCAATTC1453560.18693790487795967No Hit
AGTCACTTAGCGATGTACACTGACTGTGCTGTAGGCACCATCAATTCGTA1356210.1744180191904962No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTGTAGGCACCATCAATTCG1250580.16083326803315914No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC1118140.1438005647928134No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGTAGGCACCATCAATTC1111340.14292603759533262No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAAT1107280.1424038934156603No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1105000.14211066959062263No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA1089970.14017770727030854No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATT1035800.1332110692868479No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT974020.12526573248385361No Hit
GGAGCGAGCGCACGGGGTCGGCGGCGACCTGTAGGCACCATCAATTCGTA935020.12025006179241989No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATTCGTATG878020.11291946616647827No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATT862850.11096849887445134No Hit
CTCCCGGGGCTACGCCTGTCTGAGAGTCGCTCTGTAGGCACCATCAATTC858700.11043478007010649No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATTCG849790.10928889222752507No Hit
CGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATTCGTATGCCGTC846960.10892493458504413No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC845190.1086973002998175No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATTCGT828380.10653541762486876No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAACCTGTAGGCACCATCAAT814420.10474006473121708No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT780140.10033141880038762No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)