FastQCFastQC Report
Sat 23 Sept 2023
SRR20672858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20672858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42716615
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length10-34
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAGGGATAACAGG33809917.914931929882553No Hit
ATGTTAGGGATAACAGGGTAATGCGA20011514.684713430593693No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA8653242.025731673729297No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC7954061.8620529740008658No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3878920.908058843145694No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3383980.7921929207171494No Hit
ACCTCTAGCATTACAAGTATTAGAGGC3254100.7617878897941702No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA3175560.7434016014611644No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3000520.7024245717971801No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2618720.6130448304482927No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCG2553430.5977603796555508No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2440760.5713842260207181No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC2339320.5476370260143506No Hit
TCGCTTCTGGCGCCAAGCGTCCG2244780.5255051225383847No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC2076840.4861902095940889No Hit
ATGTTAGGGATAACAG2024150.47385543072642805No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT1881580.4404796587931885No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCC1681680.3936828796008298No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1680030.39329661303921204No Hit
ACCCCGAGTGTTACAGCCCTCCGG1678050.3928330931652707No Hit
AGAGCCACCTTACAAATAAGCGCTCTC1662320.38915068527784796No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1609840.3768650676089386No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC1528590.3578443657110939No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1508180.3530663653943553No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG1311580.3070421193252321No Hit
ATGTTAGGGATAACA1264180.2959457344642126No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1251300.29293051427412964No Hit
AGAGCCACCTTACAAATAAGCGCTCTCA1221070.28585364266339924No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1203420.2817217609588213No Hit
ATGTTAGGGATAACAGGGTAATGCG1194970.27974360796144543No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1066190.24959608807954467No Hit
CCGTCCCCGCTCGGCGGGGT1065820.24950947072936375No Hit
ACCTCTAGCATTACAAGTATTAGAGG1036390.24261987987578135No Hit
TATGTTAGGGATAACAGGGTAATGCGA1023290.23955315747748268No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1011150.23671117198776165No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1008830.23616805779203245No Hit
ACTAATCCTAGCCCTAGCCC996440.23326754706570266No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG971760.22748993570768658No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCG899100.21048016093971866No Hit
TTAGAGCCACCTTACAAATAAGCGCTCTCA893060.20906619122325118No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT889180.20815787955108336No Hit
TCGAAGCCGAGCGCACGGGGTCGGCGGCGA888060.20789568649107615No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC825600.19327374137674533No Hit
GCCGGGTTTCGCCCCCGCGGCG821140.19222965115564516No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA803360.18806733632803066No Hit
CGGTTGGCCCCGGATAGCCGGGT799990.18727841613854468No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC798120.1868406473687112No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT786790.18418828364560252No Hit
ACCATGATCACGAAGGTGGT763210.17866818332866496No Hit
TGTTAGGGATAACAGG717030.16785740162229615No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA711740.16661900761565493No Hit
ATGTTAGGGATAACAGGG709820.16616953379849972No Hit
TTCGGTTGGCCCCGGATAGCCGGGT708110.16576922118009585No Hit
CCGGGTTTCGCCCCCGCGGCG691780.16194635272481211No Hit
ATTGATCATCGACACTTCGAACGCA663440.1553119319028439No Hit
ATAACAGGGTAATGCGA644640.1509108341098657No Hit
AGCCGAGCGCACGGGGTCGGCGGCGA617340.14451987827218987No Hit
AAGCCGAGCGCACGGGGTCGGCGGCGA602470.14103879719870124No Hit
TCGTCCCCGCTCGGCGGGGT587970.1376443334753936No Hit
ATCCGAGTCACGGC585510.13706844514716346No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCA584340.1367945470398345No Hit
ATGTTAGGGATAACAGGGTAATGC572690.13406727101386662No Hit
ATGTTAGGGATAA572360.13399001770154306No Hit
GGTTGGCCCCGGATAGCCGGGTCCC571920.13388701328511166No Hit
TCACCTCTAGCATTACAAGTATTAGAGGC546920.1280344896242364No Hit
TAGAGCCACCTTACAAATAAGCGCTCTC546750.1279946924633424No Hit
TCCTTCTGATCGAGGCC538040.12595567321989348No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGA535860.12544533315666515No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCT531150.12434271769895625No Hit
TCGACGAAGCCGAGCGCACGGG529260.12390026691019408No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC520170.12177228930709982No Hit
TATGTTAGGGATAACAGG508850.1191222665934555No Hit
GTTGGCCCCGGATAGCCGGGTCCC507910.11890221170380659No Hit
AAGTGTGCTTGGAATAATA505930.11843869182986526No Hit
ACTAATCCTAGCCCTAGCC501430.11738523757090771No Hit
GTTAGGGATAACAGG501080.11730330223965546No Hit
ATGTTAGGGATAACAGGGTAA493540.11553818110353548No Hit
TCGGTTGGCCCCGGATAGCCGGGT482030.11284367921006849No Hit
ATGTTAGGGATAAC481310.11267512652863529No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCC473660.11088425428840745No Hit
TCTCGCTTCTGGCGCCAAGCGTCCG462030.10816166028136827No Hit
ACGATGCCGACTGGCGATGCGGCGGCG447730.1048140167473476No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT434840.10179645554780031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)