FastQCFastQC Report
Sat 23 Sept 2023
SRR20672859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20672859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28472236
Total Bases695 Mbp
Sequences flagged as poor quality0
Sequence length10-34
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAGGGATAACAGG16887775.9313114712873265No Hit
ATGTTAGGGATAACAGGGTAATGCGA7490392.630769848915273No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC6509402.286227186372015No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5616891.9727604112300838No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4380881.5386497920289786No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3448121.2110464383619186No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC3152771.1073138056315632No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA2715870.9538660750072456No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCC2384330.8374228142812529No Hit
ACCTCTAGCATTACAAGTATTAGAGGC2313630.8125916067849395No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2275640.7992487839732714No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2023060.7105378025104877No Hit
TCGCTTCTGGCGCCAAGCGTCCG1667020.5854896678996339No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC1611600.566025091952736No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCG1586120.5570760231124805No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC1456530.5115615085516992No Hit
ATGTTAGGGATAACAG1373590.4824313763063779No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1247510.4381496416368563No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCC1224010.4298959870942346No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG1178310.4138452631539019No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG1080170.3793765969065443No Hit
TCGGTCACGCGTCT1026170.36041075242562615No Hit
TTAGAGCCACCTTACAAATAAGCGCTCTCA1021950.3589286068013766No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC935750.3286534995003554No Hit
ATGTTAGGGATAACA922950.3241578919196933No Hit
GCGGGGCGGACTGT914000.3210144788066522No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT888960.312219946476982No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC882220.3098527281102896No Hit
CCGTCCCCGCTCGGCGGGGT879860.30902385046260505No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC856370.30077370811340565No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA834330.29303283381045314No Hit
TCACCTCTAGCATTACAAGTATTAGAGGC788760.2770277683846116No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC773080.27152064909830054No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT766150.26908669905658267No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA743350.26107889805352835No Hit
ATGTTAGGGATAAC743220.2610332395390373No Hit
GCCGGGTTTCGCCCCCGCGGCG740030.2599128498372941No Hit
CTCGCTTCTGGCGCCAAGCGTCCG726740.2552451447789348No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG703090.24693880733497714No Hit
ATAACAGGGTAATGCGA696190.24451539387352647No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC675050.2370906169785892No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC671880.2359772516636909No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG656060.23042096166946635No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT604680.2123753118652149No Hit
TCTCGCTTCTGGCGCCAAGCGTCCG587440.2063202904050107No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCG585490.20563541268764418No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCC567030.19915190362990812No Hit
ATGTTAGGGATAACAGGGTAATGCG561500.1972096606673252No Hit
TTCGGTCACGCGTCT548780.1927421506340422No Hit
TCTGAGCGTCGCT546140.19181493157053067No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC535940.18823249427969058No Hit
GCCGGGTTTCGCCCCCGCGGCGTC509980.17911484015516027No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG505290.1774676214400583No Hit
ACCCCGAGTGTTACAGCCCTCCGG485310.17045025898211857No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG478780.16815679667729644No Hit
AGAGCCACCTTACAAATAAGCGCTCTCA475590.1670364069755533No Hit
AGAGCCACCTTACAAATAAGCGCTCTC468460.16453221306538762No Hit
ACTAATCCTAGCCCTAGCCC467670.16425474978501864No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA463800.16289553093055284No Hit
TGTTAGGGATAACAGG455970.1601454834808197No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCC450270.15814353323005612No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCA449840.15799250891289326No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT432470.15189182893819791No Hit
ACCCTACGCCGGCAGGCGCGGGT424870.14922256193717978No Hit
TCCTTCTGATCGAGGCC420930.14783875772875724No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCC414600.14561553929238294No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG407340.14306568686772617No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCA406000.1425950529491256No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCC381660.13404637415902285No Hit
TGCGTGCATTTATCAGA378660.13299271613230518No Hit
CCCCCGCCCCTTGCCTCTCGGC377910.13272930162562574No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC377360.13253613098739417No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCC376630.13227974086755953No Hit
TGCGGGGCGGACTGT375850.13200578978061295No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC365520.12837769397528173No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC362050.12715896285771164No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGC355950.12501652487005235No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC343810.12075272205526814No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC339180.11912657650070053No Hit
CGTCCCCGCTCGGCGGGGT332390.11674179716689621No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT330960.1162395535074941No Hit
CCCCCGCCCCTTGCCTCTCGGCG326850.1147960420108909No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGT320720.11264306744296444No Hit
CGCTTCTGGCGCCAAGCGTCCG319440.11219350668489822No Hit
TGTGCATTTATCAGA319310.11214784817040714No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA317590.11154375090175565No Hit
GGTAATGCGA313330.11004755650381656No Hit
ACCTCTAGCATTACAAGTATTAGAGG313110.10997028824852394No Hit
TATGTTAGGGATAACAGGGTAATGCGA310540.10906765453896912No Hit
AAGTGTGCTTGGAATAATA307700.10807019160700973No Hit
ATGCGTGCATTTATCAGA307650.10805263063989777No Hit
ATGTTAGGGATAACAGGGTAATG305930.10744853337124628No Hit
CCGGGTTTCGCCCCCGCGGCG305780.1073958504699104No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC299430.10516560764669133No Hit
ACCTCTAGCATTACAAGTATTAGAGGCA296950.10429458367793804No Hit
TCGACTGCATAATTTGTGGTAGTGGGG292780.10282999902080046No Hit
CGGTTGGCCCCGGATAGCCGGGTCC286170.10050843916859918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)