FastQCFastQC Report
Sat 23 Sept 2023
SRR20672860_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20672860_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36340496
Total Bases913.8 Mbp
Sequences flagged as poor quality0
Sequence length10-34
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAGGGATAACAGG17340564.771690512974837No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC10959623.015814643806733No Hit
ATGTTAGGGATAACAGGGTAATGCGA8888622.44592699009942No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA6137291.6888294535110364No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT5312131.4617659593859147No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT5175111.4240614657543476No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3711471.0213041671197882No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC3584860.9864642463878314No Hit
ACCTCTAGCATTACAAGTATTAGAGGC3428600.9434653836315277No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC3187190.8770353602218308No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC3043360.8374569240882128No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCC2908430.800327546437451No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2657020.7311457719234211No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG2175880.5987480192895551No Hit
TCGCTTCTGGCGCCAAGCGTCCG1908670.5252184780306796No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC1885020.5187105866689327No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCG1730970.4763198609066866No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1620340.4458772384394533No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1514330.41670592498242187No Hit
GCCGGGTTTCGCCCCCGCGGCG1426460.39252628802864986No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1403270.38614497721770225No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1372950.3778016678693653No Hit
CCGTCCCCGCTCGGCGGGGT1313900.36155257759828047No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1307820.3598795129268461No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCG1302820.3585036373746798No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG1259270.3465197613153106No Hit
TTAGAGCCACCTTACAAATAAGCGCTCTCA1230660.3386470014058146No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCC1221280.3360658588699505No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1158110.3186830471438805No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1153590.3174392556447221No Hit
TCACCTCTAGCATTACAAGTATTAGAGGC1147590.31578820498212246No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC1132570.3116550748234146No Hit
ACCCCGAGTGTTACAGCCCTCCGG1008110.2774067805788892No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA999260.2749714808515547No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC970510.267060196426598No Hit
ATGTTAGGGATAACAG960060.2641846165225703No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG945860.26027712995441776No Hit
TCGACTGCATAATTTGTGGTAGTGGGG887610.2442481797716795No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCC867870.23881622309172662No Hit
AGAGCCACCTTACAAATAAGCGCTCTC867510.2387171600519707No Hit
ATTGATCATCGACACTTCGAACGCA851160.23421804699638663No Hit
GCCGGGTTTCGCCCCCGCGGCGTC847510.23321365784330517No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC835670.22995558453577516No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC808500.2224790767853031No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG807770.2222781989546868No Hit
AGAGCCACCTTACAAATAAGCGCTCTCA799940.22012357783999428No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA776980.21380555730444623No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA732680.20161529991225216No Hit
GCGGGGCGGACTGT727300.20013485781812113No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG727220.20011284380928648No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA658560.1812193207269378No Hit
CTCGCTTCTGGCGCCAAGCGTCCG658140.1811037471805558No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT638840.1757928675491936No Hit
ATGTTAGGGATAACA616660.16968948359978356No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCC614230.1690208080814307No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCA600630.16527842657953817No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT596380.16410893236019675No Hit
ACTAATCCTAGCCCTAGCCC581050.15989049791725463No Hit
GGTTGGCCCCGGATAGCCGGGTCCC576540.15864945816920054No Hit
CGGTTGGCCCCGGATAGCCGGGT555130.15275795905482412No Hit
TTCGGTTGGCCCCGGATAGCCGGGT553260.1522433815983139No Hit
CCGGGTTTCGCCCCCGCGGCG550230.15140960101370107No Hit
ACCTCTAGCATTACAAGTATTAGAGG548610.1509638173347992No Hit
TCTCGCTTCTGGCGCCAAGCGTCCG546150.15028688656313333No Hit
ACCCTACGCCGGCAGGCGCGGGT536170.14754063896100922No Hit
ATGTTAGGGATAACAGGGTAATGCG529660.1457492489920886No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC527680.14520440227343073No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG525470.1445962652793732No Hit
AAGTGTGCTTGGAATAATA510760.1405484394048997No Hit
CGGTTGGCCCCGGATAGCCGGGTCC509680.14025125028563176No Hit
GGGGGGTGGGGGCCGGGCCGCC501620.13803333889553956No Hit
CGTCCCCGCTCGGCGGGGT500620.1377581637851063No Hit
TCTGAGCGTCGCT496780.1367014913610425No Hit
TCCTTCTGATCGAGGCC494430.1360548298515243No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCT493610.13582918626096901No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC480890.13232895885625776No Hit
ATAACAGGGTAATGCGA476310.13106865685047336No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT475280.13078522648672708No Hit
TGTTAGGGATAACAGG474700.1306256249226758No Hit
CGCTTCTGGCGCCAAGCGTCCG473870.13039722958101618No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC468960.12904611978878877No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGT468510.12892229098909383No Hit
AGTGTGCTTGGAATAATA466070.12825086371963662No Hit
GTTGGCCCCGGATAGCCGGGTCCC450080.12385081370380854No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCC442820.12185304240206297No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC438100.12055421588081792No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCC436760.12018548123283732No Hit
TCGGTCACGCGTCT436410.12008916994418568No Hit
GCCGGGTTTCGCCCCCGCGGCGT434770.11963788276307512No Hit
ACCATGATCACGAAGGTGGT428880.11801710136262313No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC424470.11680357912561237No Hit
TCGAAGCCGAGCGCACGGGGTCGGCGGCGA420900.11582120398136558No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT416460.11459942649104185No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC409520.1126897112246349No Hit
TGGGGGTGGGGGCCGGGCCGCC398350.10961600524109522No Hit
ATGTTAGGGATAACAGGG395460.10882074917194305No Hit
ATGCTTAGATG391830.10782186352107027No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGC388390.1068752611411798No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGA388260.10683948837682347No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC375750.10339704774530319No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGC374150.10295676756860997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)