FastQCFastQC Report
Sat 23 Sept 2023
SRR20672877_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20672877_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30760995
Total Bases751.7 Mbp
Sequences flagged as poor quality0
Sequence length10-34
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAGGGATAACAGG9480183.081883404616788No Hit
ATGTTAGGGATAACAGGGTAATGCGA4878111.5858102119258495No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4853701.5778748379238057No Hit
ACCTCTAGCATTACAAGTATTAGAGGC4409151.4333574060266907No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC4097931.332183825653234No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCC3411461.1090213434253346No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3038020.9876208490655131No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA2759780.897168638400676No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2566090.8342025347359538No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2094920.6810312865367326No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1933110.628428956865667No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1843990.5994572022133875No Hit
TCGCTTCTGGCGCCAAGCGTCCG1817250.5907643754696492No Hit
TTAGAGCCACCTTACAAATAAGCGCTCTCA1765450.5739248681650253No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCG1750950.5692111064677849No Hit
TCACCTCTAGCATTACAAGTATTAGAGGC1658800.5392543381642889No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC1578460.5131368474914416No Hit
GCGGGGCGGACTGT1400200.4551868364466104No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCC1290740.41960281193765026No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1152190.3745620062029853No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA1129000.36702323835753686No Hit
ATGTTAGGGATAACAG1088400.3538247056052641No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1040900.3383830724591321No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC1016170.3303436706127354No Hit
ACTCTAGATAACCTCGGGCCGATCGCAC981230.3189851303574543No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC918040.29844288196789476No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG906600.29472388653227893No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT903950.2938624059462316No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCA891610.28985083219837326No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT853270.27738699609684275No Hit
ATGCTTAGATG826600.2687169254440567No Hit
ACTAATCCTAGCCCTAGCCC811310.2637463450060702No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCC791970.2574591621629924No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC748650.24337639273372008No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC744670.24208254641958105No Hit
TCGGTCACGCGTCT725960.23600016839507304No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA690570.22449533898367072No Hit
ATGTTAGGGATAACA686160.22306170525368246No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC679050.2207503365869667No Hit
ATAACAGGGTAATGCGA674780.2193622150388828No Hit
AAGTGTGCTTGGAATAATA668290.21725240032060084No Hit
AGAGCCACCTTACAAATAAGCGCTCTCA653880.2125678964545848No Hit
TCTCGCTTCTGGCGCCAAGCGTCCG652350.21207051332377252No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCG643240.20910897062985123No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCA642160.2087578766551602No Hit
TCCTTCTGATCGAGGCC629490.20463902419281302No Hit
TGCGGGGCGGACTGT627390.20395634146424718No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG608530.1978252003876988No Hit
TTCGGTCACGCGTCT604690.1965768662554641No Hit
ACCTCTAGCATTACAAGTATTAGAGGCA589190.19153801754462105No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCC582100.18923315061817733No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA576070.18727287592615258No Hit
ATCCGAGTCACGGC568870.1849322494282126No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC560420.1821852641632691No Hit
TCGGGGCGGACTGT544930.17714966632256207No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC528560.17182799190988457No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG517440.16821302431862167No Hit
ACCCCGAGTGTTACAGCCCTCCGG508820.16541077426136575No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC498130.16193559408595204No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG495220.16098959087636794No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT494860.16087255955147095No Hit
TCTGAGCGTCGCT483620.15721858151857573No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG481940.15667243533572303No Hit
ACCTCTAGCATTACAAGTATTAGAGG476740.1549819828649886No Hit
ATGTTAGGGATAACAGGGTAATGCG469440.1526088476656883No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG437520.14223207019148762No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCC425810.1384253012621991No Hit
AGAGCCACCTTACAAATAAGCGCTCTC418970.1362017060891561No Hit
ATGCGTGCATTTATCAGA407000.13231041453633083No Hit
CCGTCCCCGCTCGGCGGGGT397710.12929035617996104No Hit
GCCGGGTTTCGCCCCCGCGGCG385630.12536330505563945No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT382170.12423850398857385No Hit
GCCGGGTTTCGCCCCCGCGGCGTC375700.12213519101056387No Hit
TCGCTTCTGGCGCCAAGCGTCC370420.12041873157874118No Hit
TCGCCGCGGCCCGGGTTCGTTTCCCGGTCAGGGA370350.12039597548778899No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC361050.11737266626128315No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCC360460.11718086492325751No Hit
ATCTCGCTGGGGCC354990.11540263895885032No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCG353540.11493126278912628No Hit
CTCGCTTCTGGCGCCAAGCGTCCG350450.1139267439170937No Hit
TTGGGGGTGGGGGCCGGGCCGCC345820.11242159104411285No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT341850.11113099560010982No Hit
TCCTTCTGATCGAGGCCC335050.10892040390761092No Hit
ATCCGAGTCACGG328600.106823592669873No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCGT310860.10105654904855972No Hit
ATGTTAGGGATAACAGGTAATGCGA310130.10081923552862969No Hit
TTAGAGCCACCTTACAAATAAGCGCTCTC307910.1000975423584315No Hit
TTCGGTTGGCCCCGGATAGCCGGGT307710.10003252495571097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)