FastQCFastQC Report
Sat 23 Sept 2023
SRR20672878_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20672878_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28396821
Total Bases709.6 Mbp
Sequences flagged as poor quality0
Sequence length10-34
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAGGGATAACAGG7024592.473724083410604No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA4875241.7168259785135809No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC4783081.6843716414594436No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4486721.580007846652976No Hit
ATGTTAGGGATAACAGGGTAATGCGA3707291.3055299394252617No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCC3609591.2711246797660907No Hit
ACCTCTAGCATTACAAGTATTAGAGGC3581661.261289071759124No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3546641.2489567054002277No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2944581.0369400152221264No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2909411.0245548260490143No Hit
TCGCTTCTGGCGCCAAGCGTCCG2393600.842911254044951No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC2050980.7222569033343557No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1966660.6925634387032267No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCG1718240.6050818153201022No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1564150.5508186990367689No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC1504340.5297564822484884No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1503940.5296156214105797No Hit
TCACCTCTAGCATTACAAGTATTAGAGGC1300550.45799140685501377No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1120810.3946955893407928No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG1110670.3911247670998102No Hit
TTAGAGCCACCTTACAAATAAGCGCTCTCA1070410.3769471237643115No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCC1032270.3635160428697283No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1030290.3628187817220808No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCG983540.34635567129151534No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC950860.3348473408343843No Hit
ACCCCGAGTGTTACAGCCCTCCGG931200.3279240306511775No Hit
TCTCGCTTCTGGCGCCAAGCGTCCG760530.2678222326365335No Hit
AAGTGTGCTTGGAATAATA756800.266508705323036No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA742120.26133911257179104No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG740940.2609235730999607No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT739440.26039534495780353No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC727040.25602865898263755No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC703410.2477073049831881No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA672590.23685397742233189No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCC659270.23216331151997613No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG652870.2299095381134388No Hit
TCCTTCTGATCGAGGCC644650.22701484789441748No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC641390.22586683206546254No Hit
ACTAATCCTAGCCCTAGCCC636830.22426101851330474No Hit
GCCGGGTTTCGCCCCCGCGGCG588160.20712177606077806No Hit
AGAGCCACCTTACAAATAAGCGCTCTCA573020.20179019334593826No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT571980.20142395516737593No Hit
CCGTCCCCGCTCGGCGGGGT513800.18093574629357279No Hit
GCGGGGCGGACTGT507270.1786361931147152No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC503780.17740718230396282No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC499870.17603026761340643No Hit
TCGACTGCATAATTTGTGGTAGTGGGG495760.17458292250389576No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT487810.1717833133504627No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA478640.16855407864140848No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG466720.16435642567173275No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCC461150.16239493850385575No Hit
ATGCTTAGATG460930.16231746504300607No Hit
TTCGGTTGGCCCCGGATAGCCGGGT460900.1623069004801629No Hit
TCTGAGCGTCGCT452420.15932065071650098No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCA448890.15807755382195773No Hit
AGAGCCACCTTACAAATAAGCGCTCTC433560.15267906220911137No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCC426850.1503161216531949No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCC426490.15018934689907718No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG413810.14572405833737515No Hit
CTCGCTTCTGGCGCCAAGCGTCCG410570.14458308555031565No Hit
ATAACAGGGTAATGCGA406890.14328716584155668No Hit
ATCCGAGTCACGGC406060.1429948796028964No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG393700.13864227971152124No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCA390740.13759990951099774No Hit
AGTGTGCTTGGAATAATA382790.13480030035756466No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT357690.1259612827788012No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGA354510.12484143911742795No Hit
GCCGGGTTTCGCCCCCGCGGCGTC351430.1237568106655319No Hit
TCGAAGCCGAGCGCACGGGGTCGGCGGCGA349980.12324619012811329No Hit
ACCTCTAGCATTACAAGTATTAGAGGCA347810.12248202008245923No Hit
CCGGGTTTCGCCCCCGCGGCG341550.12027754796918994No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC340400.11987257306020276No Hit
ACTCTAGATAACCTCGGGCCGATCGCAC338430.119178833433503No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG332150.11696731827833826No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC326880.11511147673889272No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA317350.11175546727572076No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC317340.11175194575477304No Hit
ATAACCTCGGGCCGATCGCAC313370.11035390193853037No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT313080.11025177783104666No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT311380.10965311926993519No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC305780.10768106753921505No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCC296930.10456452150048767No Hit
ACCTCTAGCATTACAAGTATTAGAGG292730.10308548270244756No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC290370.10225440375878694No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCG286630.10093735492434171No Hit
GGTTGGCCCCGGATAGCCGGGTCCC285270.10045842807545254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)