FastQCFastQC Report
Sat 23 Sept 2023
SRR20672879_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20672879_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37395586
Total Bases936.8 Mbp
Sequences flagged as poor quality0
Sequence length10-34
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC8696232.325469642326236No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT7781382.080828469969691No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT6489291.7353090816654138No Hit
ATGTTAGGGATAACAGG6260441.6741120195308612No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT5474911.4640524686523164No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC4409841.1792407799145066No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCC4240911.1340669992442423No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA4183871.1188138621493993No Hit
ACCTCTAGCATTACAAGTATTAGAGGC3650590.9762087964071482No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3377520.9031868092667407No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC2615770.6994862976609058No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC2557380.6838721553928852No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2285370.6111336241662318No Hit
GCGGGGCGGACTGT2240350.5990947701688643No Hit
TCGCTTCTGGCGCCAAGCGTCCG2194680.5868820988658929No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCC2059260.5506692688276097No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCG1887340.5046959285515675No Hit
ATGTTAGGGATAACAGGGTAATGCGA1870510.5001953973926228No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1538440.4113961471281664No Hit
TTAGAGCCACCTTACAAATAAGCGCTCTCA1495870.400012450667306No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC1487270.39771271400854635No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1358740.36334234740966487No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1349290.36081531119742316No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG1267280.33888491545499516No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG1241450.3319776831415344No Hit
TCACCTCTAGCATTACAAGTATTAGAGGC1238870.33128776214390654No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1221420.3266214360165395No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1168850.3125636271617725No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1168180.3123844616313808No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1152580.3082128462968865No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA1143500.30578475224321927No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1077750.2882024632532834No Hit
TCGGTCACGCGTCT1037250.2773723080579617No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCC968820.2590733569464589No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCG958880.2564152892269157No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC948780.25371443570907004No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC943910.25241214297323755No Hit
GCCGGGTTTCGCCCCCGCGGCG942160.2519441733043039No Hit
TCTGAGCGTCGCT938000.2508317425484387No Hit
CTCGCTTCTGGCGCCAAGCGTCCG911310.24369453656910203No Hit
TCCTTCTGATCGAGGCC868230.23217446037615241No Hit
GCCGGGTTTCGCCCCCGCGGCGTC866730.23177334351706644No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG845120.2259945866338343No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG838490.22422165011667416No Hit
CCGTCCCCGCTCGGCGGGGT824030.22035488359508523No Hit
TCTCGCTTCTGGCGCCAAGCGTCCG804220.2150574669427563No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC787580.2106077439192957No Hit
AGAGCCACCTTACAAATAAGCGCTCTCA758530.20283944741499704No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT743250.19875340367710778No Hit
TCGACTGCATAATTTGTGGTAGTGGGG726050.19415393035958842No Hit
ACCCCGAGTGTTACAGCCCTCCGG722850.1932982143935383No Hit
TGCGGGGCGGACTGT714820.19115090214123132No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCC666490.17822691694148074No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCA657690.17587369803484293No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT653870.1748521871003706No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA652300.1744323514545273No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCC651230.17414622142837927No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG649690.17373440811971766No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCC646490.17287869215366755No Hit
ATGTTAGGGATAACAG645590.17263802203821596No Hit
ACTCTAGATAACCTCGGGCCGATCGCAC633760.16947454707622447No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA629950.16845571025414602No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT629750.16840222800626792No Hit
ACTAATCCTAGCCCTAGCCC624280.166939488526801No Hit
AAGTGTGCTTGGAATAATA610840.16334548146939054No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG594170.15888773610874823No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCC562850.15051241609103277No Hit
AGAGCCACCTTACAAATAAGCGCTCTC562510.15042149626963996No Hit
TTCGGTCACGCGTCT544310.14555461171272993No Hit
ATCCGAGTCACGGC543330.1452925486981271No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC538970.14412663569438383No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC520790.13926509936226164No Hit
ATGCTTAGATG487150.13026938526915985No Hit
TCCTTCTGATCGAGGCCC486360.13005813039004124No Hit
AGTGTGCTTGGAATAATA479860.12831995733400195No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC454670.12158386821375122No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT452460.12099288937469786No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCC451880.12083779085585128No Hit
TCGGGGCGGACTGT447490.11966385551492628No Hit
TGGGGGTGGGGGCCGGGCCGCC447030.11954084634480658No Hit
TCACCTCTAGCATTACAAGTATTAGAGGCA444490.1188616217967543No Hit
ATGCGTGCATTTATCAGA443330.11855142475906114No Hit
CCCCCGCCCCTTGCCTCTCGGCG442930.11844446026330487No Hit
TCGCTTCTGGCGCCAAGCGTCC438300.11720634622492611No Hit
ATTGATCATCGACACTTCGAACGCA431210.11531040053764635No Hit
TGCGTGCATTTATCAGA429620.11488521666701519No Hit
GCCGGGTTTCGCCCCCGCGGCGT427890.11442259522286935No Hit
TTCGGTTGGCCCCGGATAGCCGGGT426540.11406159004969196No Hit
ACCTCTAGCATTACAAGTATTAGAGG418230.11183940265035558No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC416410.11135271419466458No Hit
CTTCTGGCGCCAAGCGTCCG412860.11040340429482774No Hit
TAACGATGCCGACTGGCGATGCGGCGGCGTTA411140.1099434569630758No Hit
CGGTTGGCCCCGGATAGCCGGGTCC411070.10992473817631844No Hit
ATGTTAGGGATAACA406400.10867592768836407No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT405660.10847804337121497No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT395120.10565952890803744No Hit
TCCCGTGGATCGCCTCAGC393810.10530922018443568No Hit
GTTGGCCCCGGATAGCCGGGTCCC393000.10509261708052925No Hit
CCGGGTTTCGCCCCCGCGGCG392160.10486799163944108No Hit
CCCCCGCCCCTTGCCTCTCGGC391780.10476637536847262No Hit
TCGGTTGGCCCCGGATAGCCGGGTCC386120.10325282775352151No Hit
TCGATGCCGACTGGCGATGCGGCGGCG382990.10241583057422875No Hit
CGCTTCTGGCGCCAAGCGTCCG382160.10219387924553448No Hit
ACCCTACGCCGGCAGGCGCGGGT377360.10091030529645931No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT376060.10056267068525146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)