FastQCFastQC Report
Sat 23 Sept 2023
SRR20672880_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20672880_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24415726
Total Bases604.5 Mbp
Sequences flagged as poor quality0
Sequence length10-34
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAGGGATAACAGG10639034.357449784618323No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC6757702.767765332884224No Hit
ATGTTAGGGATAACAGGGTAATGCGA5197842.1288902078930603No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4365811.788113939352039No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA3929451.6093930608493887No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3751451.5364892282949112No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT3319001.3593697766759014No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCC2349820.9624206955795621No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2331170.9547821760450621No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC2145500.8787369255372542No Hit
TCGCTTCTGGCGCCAAGCGTCCG2104490.8619403740032141No Hit
ACCTCTAGCATTACAAGTATTAGAGGC2079560.8517297417246572No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT2031390.8320006540047182No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1706340.6988692451741964No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC1589490.651010746106833No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCG1384410.5670157012738429No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG1357710.5560801263906713No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1143620.46839483699972717No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG1067480.43721001783850294No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1047520.42903495886216936No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC986100.40387904090994464No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCC982930.4025806973751262No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC980980.4017820317937709No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCG864250.3539726813775679No Hit
CCGTCCCCGCTCGGCGGGGT856750.35090089068004776No Hit
GCGGGGCGGACTGT852200.34903733765688555No Hit
GCCGGGTTTCGCCCCCGCGGCG839720.3439258779362121No Hit
ACCCCGAGTGTTACAGCCCTCCGG778080.3186798541235268No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA742650.30416871486844177No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT698050.28590179952052214No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC692060.2834484626834361No Hit
TCACCTCTAGCATTACAAGTATTAGAGGC671750.2751300534745516No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA616550.25252167394080355No Hit
TCCTTCTGATCGAGGCC616370.2524479509640631No Hit
CTCGCTTCTGGCGCCAAGCGTCCG593820.2432121002668526No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC556140.22777942380251154No Hit
TCGGTCACGCGTCT551260.22578071198865848No Hit
TTAGAGCCACCTTACAAATAAGCGCTCTCA544060.22283179291903912No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG530410.21724113384955254No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCG529340.21680289171003966No Hit
TCTGAGCGTCGCT529180.21673736017515924No Hit
ATAACAGGGTAATGCGA506290.20736225496632787No Hit
TCTCGCTTCTGGCGCCAAGCGTCCG492850.20185760603637182No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC488310.19999814873413965No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC483760.1981345957109774No Hit
CCGGGTTTCGCCCCCGCGGCG472330.19345318668795677No Hit
CGCTTCTGGCGCCAAGCGTCCG464160.19010698268812487No Hit
GCCGGGTTTCGCCCCCGCGGCGTC443980.18184181785133074No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCC437570.17921646073518355No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCC426390.17463744473541357No Hit
ACTAATCCTAGCCCTAGCCC413480.16934986901474894No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT412070.16877237236361517No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGA408730.1674044015729862No Hit
AAGTGTGCTTGGAATAATA405210.16596270780561675No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC404500.16567191161958486No Hit
GGTTGGCCCCGGATAGCCGGGTCCC400740.16413192054989478No Hit
CGGTTGGCCCCGGATAGCCGGGT393660.16123215013143577No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC390660.16000343385242774No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT384400.15743951255023095No Hit
ATCCGAGTCACGGC379650.1554940451084682No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCG377200.15449059348061162No Hit
AGAGCCACCTTACAAATAAGCGCTCTCA376460.15418751013178963No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG371090.15198810799236523No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG365650.14976003580643066No Hit
ATGTTAGGGATAACA364720.14937913375993817No Hit
AGTGTGCTTGGAATAATA361820.1481913746902304No Hit
GTTGGCCCCGGATAGCCGGGTCCC359100.1470773385972631No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC355670.1456725063182639No Hit
AGAGCCACCTTACAAATAAGCGCTCTC354930.1453694229694419No Hit
CGTCCCCGCTCGGCGGGGT354190.1450663396206199No Hit
TTCGGTTGGCCCCGGATAGCCGGGT348300.14265395999283412No Hit
ATGTTAGGGATAACAG344860.1412450319929049No Hit
TCGACTGCATAATTTGTGGTAGTGGGG337890.1383903145046762No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCC332280.13609261506293116No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC330780.13547825692342713No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC326010.13352459803980435No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT321760.13178391664454295No Hit
ATGCTTAGATG318180.13031764855159336No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCT310890.1273318679936038No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGA299330.1225972145984928No Hit
ATTGATCATCGACACTTCGAACGCA296300.12135621115669468No Hit
TGCGGGGCGGACTGT294390.12057392845905954No Hit
TCGGGGCGGACTGT281860.11544199013373595No Hit
TGTTAGGGATAACAGG281500.11529454418025496No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCC280890.11504470520352333No Hit
TCGAAGCCGAGCGCACGGGGTCGGCGGCGA277380.11360710715708393No Hit
AAGCCGAGCGCACGGGGTCGGCGGCGA272750.11171078836648152No Hit
ATGTTAGGGATAACAGGG272740.11170669264555148No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT266970.10934346166892601No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGT266950.10933527022706595No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA266790.10926973869218551No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCA266410.1091141012968445No Hit
GGGGGGTGGGGGCCGGGCCGCC265240.10863490194803135No Hit
CTTCTGGCGCCAAGCGTCCG264440.10830724427362921No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCC263260.10782394920388605No Hit
ACCCTACGCCGGCAGGCGCGGGT252320.10334323050643672No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT251210.10288860548320372No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC248040.10159026194838525No Hit
TCGTCCCCGCTCGGCGGGGT247600.10141005022746405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)