FastQCFastQC Report
Sat 23 Sept 2023
SRR20672881_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20672881_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39250785
Total Bases988.6 Mbp
Sequences flagged as poor quality0
Sequence length10-34
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTTAGGGATAACAGG12989963.309477759489396No Hit
ATGTTAGGGATAACAGGGTAATGCGA9245962.3556114864963846No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA8589532.18837151919382No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT6444031.641758247637595No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC6376611.6245815211084313No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT4584951.1681167650532338No Hit
ACCTCTAGCATTACAAGTATTAGAGGC4026261.0257782105504387No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3826010.9747601226319423No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT3754110.9564420176564621No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCC3644720.9285725113523208No Hit
TCGCTTCTGGCGCCAAGCGTCCG2643010.6733648766515116No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2492380.634988574113868No Hit
CGGTTGGCCCCGGATAGCCGGGTCCC2443940.6226474196630717No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCC2418800.6162424522210194No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2304570.5871398495596967No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC2081730.5303664627344395No Hit
ATGTACACTAGGGATAACAGGGTAATCAACGCGA2054140.5233373039545578No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCG2046570.5214086801066526No Hit
TTAGAGCCACCTTACAAATAAGCGCTCTCA1727690.4401669928384872No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCCG1625020.4140095542038204No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAT1380330.35166939973302447No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCCG1334140.33990148222513256No Hit
AGAGCCACCTTACAAATAAGCGCTCTCA1315740.33521367789204726No Hit
ATGTTAGGGATAACAG1298780.3308927452024208No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCG1283370.32696670907346187No Hit
ACCCCGAGTGTTACAGCCCTCCGG1275880.3250584669835266No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1274640.32474254973499256No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT1237480.31527522315795725No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGA1180320.30071245708843786No Hit
TCGACTGCATAATTTGTGGTAGTGGGG1166620.29722208103608627No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1153060.29376737306018214No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTT1103210.28106699012516567No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1092990.2784632205445063No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT1022540.2605145349322313No Hit
AGAGCCACCTTACAAATAAGCGCTCTC998560.2544051029807429No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC977140.24894788728429254No Hit
ACGATGCCGACTGGCGATGCGGCGGCGTTA975190.24845108193377535No Hit
GCCGGGTTTCGCCCCCGCGGCG952650.24270852162574583No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCC914810.23306795010596604No Hit
ACTAATCCTAGCCCTAGCCC874850.22288726199998296No Hit
ACCTCTAGCATTACAAGTATTAGAGG824520.21006458851714685No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGC822560.2095652354468834No Hit
CCGTCCCCGCTCGGCGGGGT821800.209371608746169No Hit
ATGTTAGGGATAACAGGGTAATGCG804780.20503538973806512No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCT774190.19724191503431077No Hit
TAGAGCCACCTTACAAATAAGCGCTCTCA769260.19598588919941345No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG754690.19227386152913883No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCC744690.18972614178289682No Hit
ATTGATCATCGACACTTCGAACGCA744330.1896344238720321No Hit
ATGTTAGGGATAACA728900.18570329230358068No Hit
TCACCTCTAGCATTACAAGTATTAGAGGC709650.1807989317920648No Hit
TCGAAGCCGAGCGCACGGGGTCGGCGGCGA708440.1804906577027695No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGC680660.17341309224770918No Hit
TCTCGCTTCTGGCGCCAAGCGTCCG679630.17315067711384627No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC658370.16773422493333573No Hit
TCCTTCTGATCGAGGCC656450.16724506274205725No Hit
TTCGGTTGGCCCCGGATAGCCGGGT654180.16666673035966034No Hit
ATGTTAGGGATAAC654070.16663870544245166No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCC647940.1650769532380053No Hit
TCTGAGCGTCGCT630850.1607229001916777No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCG622690.15864396087874422No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTA616160.15698029988444817No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCC597000.15209886885064847No Hit
TTCGGTTGGCCCCGGATAGCCGGGTCCCC592310.15090398828966095No Hit
CCGGGTTTCGCCCCCGCGGCG575280.1465652215618108No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCT573290.14605822533230864No Hit
GCGGGGCGGACTGT549770.14006598848914742No Hit
ACGAAGCCGAGCGCACGGGGTCGGCGGCGA548740.13980357335528448No Hit
ATAACAGGGTAATGCGA545700.13902906655242692No Hit
CGGTTGGCCCCGGATAGCCGGGT521560.13287887108499868No Hit
TCGATGCCGACTGGCGATGCGGCGGCG510560.13007637936413247No Hit
ATCCGAGTCACGGC508760.1296177898098089No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCC496520.12649938084040868No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCC487140.12410961971843365No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCT486890.12404592672477761No Hit
ACCCTACGCCGGCAGGCGCGGGT486060.12383446598583951No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTT483410.12315932025308539No Hit
TCGGTTGGCCCCGGATAGCCGGGTCCCC468040.1192434750031114No Hit
TATGTTAGGGATAACAGGGTAATGCGA466530.11885876932142886No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCG453630.11557221084877665No Hit
GCCGGGTTTCGCCCCCGCGGCGTC451130.11493528091221616No Hit
AAGTGTGCTTGGAATAATA439560.11198756916581414No Hit
AAGCCGAGCGCACGGGGTCGGCGGCGA437630.11149585925478943No Hit
CGGTTGGCCCCGGATAGCCGGGTCC428370.10913667076976932No Hit
CCGTGGCGCAATGAAGGTGAAGGGCC419410.10685391387713647No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC418300.10657111698530361No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGC407270.10376098210519867No Hit
TCGGTTGGCCCCGGATAGCCGGGTCC406560.10358009400321547No Hit
CTCGCTTCTGGCGCCAAGCGTCCG404570.10307309777371332No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCT396800.10109351953088326No Hit
GGTTGGCCCCGGATAGCCGGGTCCC394810.1005865233013811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)