FastQCFastQC Report
Sat 1 Jul 2023
SRR2075925.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2075925.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45615984
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG25996345.69895412099408No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5153991.1298649175254007No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA5070091.1114722418352305No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC3955880.8672135626845187No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3686580.8081772389257238No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC3582390.7853365609738903No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC3542240.7765348216537431No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA3336160.7313576749763855No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA3304120.7243338212324874No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3301140.723680541452312No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3200400.701596177339943No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG3171020.6951554525273421No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2980150.6533126633857115No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2624350.5753136882896135No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC2562120.5616715403968925No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2491740.5462427380718127No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2429080.5325063249759119No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2419210.5303426097308347No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2396870.5254452035935474No Hit
CACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2272150.49810391024339185No Hit
CACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC2214610.48548991072953723No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT2073410.45453584866217067No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA1913570.41949549964766736No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1565430.3431757604965838No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA1467870.3217885204449388No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA1451420.31818232836981No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1425080.31240803662154915No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1362450.29867820016773067No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1331000.2917836870514511No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1320030.2893788282633561No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1297920.2845318430486998No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1258440.27587698206839073No Hit
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1221770.26783813322979066No Hit
CCCGTGCCTTGGAAAGCGTCGCCTGTAGGCACCATCAATAGATCGGAAGA1141460.2502324623754691No Hit
CAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA1133170.2484151169467264No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1103580.2419283556395495No Hit
GTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1078230.23637109308000456No Hit
TCCCACGGCGCGACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1053840.23102428306709333No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1049330.23003559454072064No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG1012590.2219814002039285No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1008790.22114835887350362No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG987090.21639125443397209No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG980990.21505400387723742No Hit
CCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACA972830.21326515723085138No Hit
TTCTAGAGCTAATACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC950850.20844667079855167No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA949830.2082230649677534No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG935400.2050597001261663No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG930540.20399428410883344No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA912980.2001447562766595No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA902070.19775305077272914No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA901910.1977179753482902No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA899810.19725761040252907No Hit
GGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC887300.19451515065420927No Hit
GCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCA880380.19299813854722503No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA873900.1915775838574479No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC871080.19095937950171152No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA829610.18186826792994315No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC822240.18025260619172437No Hit
GGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA803090.17605451632918848No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA802100.1758374871404725No Hit
TTTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGC794860.17425032418461037No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATTGG785090.17210853107980745No Hit
TTCTAGAGCTAATACATCTGTAGGCACCATCAATAGATCGGAAGAGCACA780540.17111107369732506No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA772820.16941868446814606No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG766450.1680222441326707No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG751120.16466158002861453No Hit
CCCGTGCCTTGGAAAGCGTCGCTGTAGGCACCATCAATAGATCGGAAGAG750840.16460019803584638No Hit
CCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAG749070.16421217615299058No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG746620.1636750837162693No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT745660.1634646311696356No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG714910.1567235730352764No Hit
CGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA707850.15517586993190807No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG697990.15301434690085827No Hit
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCAT693950.15212869243377497No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATTAATAGATCGG689100.15106546863046952No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA687270.15066429346344912No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC685210.15021269737379775No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA682830.14969095043526848No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT682720.1496668360809667No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA682250.14956380202167732No Hit
CCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACA679810.14902890179898343No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCATCATCAATAGATCGG669180.1466985782878212No Hit
CTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC655310.1436579774317704No Hit
AGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC655030.14359659543900224No Hit
GCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA654780.1435417900883164No Hit
CAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCGG650770.14266271226331542No Hit
GCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGC635820.13938535229230173No Hit
TCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA623680.136724004462997No Hit
GCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC620050.1359282307710385No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC618270.13553801667415527No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG616790.13521356899809506No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT616350.13511711158088796No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG613680.13453179043556313No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG593180.13003775167932363No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG585880.1284374354392969No Hit
CGGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGG578300.126775737206502No Hit
CCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC576580.12639867639378338No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACTATCAATAGATCGG561300.12304897335986438No Hit
GCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACA553290.12129300992388985No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA544800.11943182021459846No Hit
GGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAA541870.11878950150456033No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGCTGTAGGCACCAT528290.1158124748553051No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC526700.11546391282494312No Hit
CCCGTGCCTTGGAAAGCGTCGCGCTGTAGGCACCATCAATAGATCGGAAG520560.1141178934120987No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG517810.11351503455455439No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA512520.11235535333404185No Hit
CCCCCCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGATCG511120.1120484433702011No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA507070.11116059668909038No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG497360.10903195686845207No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG496960.10894426830735474No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA495100.10853651649825201No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA489120.10722557250984655Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA488910.10717953601527043No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCA488380.10706334867181644No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA484070.10611850442599244No Hit
GGCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGA481600.10557702756121626No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA474310.10397890353521695No Hit
CTCCCACGGCGCGACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC471670.10340015903197441No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGTACCATCAATAGATCGG470020.10303844371744782No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCAA460240.10089445839861746No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTACTGTAGGCACCATC459670.10076950219905373No Hit
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG456500.1000745703523572No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)