FastQCFastQC Report
Sat 1 Jul 2023
SRR2075927.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2075927.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38951271
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG11452402.94018647042352No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT7058051.812020460128246No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG6647161.7065322464060289No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC4545991.167096703981752No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG4178751.0728147998046071No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA3806860.9773390963288464No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2763570.7094941779948593No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2625250.6739831416540939No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT2573150.660607454888956No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2127490.546192703185475No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG2063370.5297311094161729No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC1908010.48984537629080194No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1849820.47490619754102503No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1688890.4335904725676346No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1684220.4323915386483794No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA1675970.4302735076347059No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1639580.42093106538166625No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1602880.4115090365087188No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1534550.3939666050948633No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG1461320.3751661916244017No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA1416590.36368261256481205No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT1376440.3533748616316012No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1375850.3532233903227446No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1212840.3113736648028764No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1196630.30721205477479796No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG1196390.30715043932712743No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG1094650.2810306241354743No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1055500.27097960423422385No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATTAAT1006560.25841518753008086No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG962100.24700092584912053No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA872610.22402606579898254No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT842780.21636777911560318No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT834400.21421637306777486No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG825460.21192119764204873No Hit
TTTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG810800.20815752071350896No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA794790.2040472568918226No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG788380.20240161097695633No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG787470.20216798573787234No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA771270.19800894302011351No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT757730.19453280484736943No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA743130.19078453178074728No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATTAATAGATCGG733080.18820438490954505No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA703960.1807283772588576No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG694810.17837928831641975No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC689200.1769390272271218No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA684430.1757144202046706No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC657730.16885970165132735No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA642290.16489577451785847No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT627530.16110642448612267No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC615550.15803078672323684No Hit
CGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATCGGAAG604070.1550835144763312No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC602660.15472152372126702No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC602580.15470098523871018No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC602000.15455208124017314No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATA598870.15374851311013704No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA598760.15372027269662136No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACTATCAAT595000.1527549640164502No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG581940.1494020567390471No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA574790.1475664298605301No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA565860.14527382174512354No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA557860.14321997348944018No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA557790.14320200231720293No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACTATCAATAGATCGG553640.14213656853456721No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA552110.14174377005566777No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT536420.13771566016420875No Hit
GTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATC529980.13606231231838364No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCA500470.12848617956523165No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG491840.12627059075941321No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA490260.12586495572891573No Hit
CGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGAT489480.12566470552398665No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA474190.12173928804531181No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAA457830.11753916836243933No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA452670.11621443623752355No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATCG447420.11486659831973134No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA447220.11481525211333925No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCATCATCAATAGATCGG441840.1134340391613922No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGTACCATCAATAGATCGG432810.11111575794278958No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC426010.10936998692545874No Hit
CCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGGA425950.10935458306354112No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGTAGGCACCATCA418790.1075163888747045No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA417270.10712615770612467No Hit
TCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC417250.10712102308548545No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCATCATCAAT415700.10672308998594679No Hit
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT407640.10465383786834581No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGTACCATCAAT403690.10363975029210215No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT397890.10215071030673172No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTCTGTAGGCACCATCA392100.10066423763168088No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG391820.10059235294273196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)