FastQCFastQC Report
Sat 1 Jul 2023
SRR2075928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2075928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36330862
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG10032632.761462142021293No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT9675402.663135270503629No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG9428382.5951434898516856No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA7370592.028740743888763No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA4926411.3559848924035989No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4825121.328105014408962No Hit
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3606110.9925748527519109No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA3373720.9286099515062427No Hit
TCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3086740.8496192575887684No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2857080.7864057836007304No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA2429190.6686298827701913No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2416120.665032390368277No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2303760.6341055161311614No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC2226630.6128756317425114No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA2194970.6041612775386392No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC2095840.576875935396193No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2073380.5706938635257264No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC2070520.5699066540177329No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA2026540.5578012434717349No Hit
CCCGTGCCTTGGAAAGCGTCGCGCTGTAGGCACCATCAATAGATCGGAAG2002340.551140239942559No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1954560.5379888866936325No Hit
CCCGTGCCTTGGAAAGCGTCGCCTGTAGGCACCATCAATAGATCGGAAGA1831840.5042104423506384No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1703570.46890437116520933No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1635120.45006364010851163No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1617120.44510917467358746No Hit
TCCGGGACGGCTGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1545450.4253821448001977No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1238330.3408479545572026No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1205560.33182807498484346No Hit
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1131510.31144595468172487No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1119350.3080989380323539No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG1113770.30656305374752735No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1008820.2776757677811223No Hit
GTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1008130.27748584660611686No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC973400.2679264807975104No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC963290.26514372271156134No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA951270.26183524079335085No Hit
CCCGTGCCTTGGAAAGCGTCGCTGTAGGCACCATCAATAGATCGGAAGAG913840.25153270516950577No Hit
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAA862310.2373491716216367No Hit
CACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC819220.2254887318665877No Hit
CACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA777140.21390629267205385No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC767120.2111483069132794No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA761940.20972252186034013No Hit
ATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATAGATCGGAAG760580.20934818447192363No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCANTAGATCGGAAGAGCACACGT748570.20604245503451032No Hit
TTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA739890.20365330170255802No Hit
TAGGAATAATGGAATCTGTAGGCACCATCNATAGATCGGAAGAGCACACG725380.19965945206584967No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA715630.1969757832885991No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG705460.19417651031786695No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGNAGGCACCATCAATAGATCGG698390.19223050639426062No Hit
TAGGAATAATGGAATCTGTAGGCACCATCGATAGATCGGAAGAGCACACG698360.19222224895186907No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGAGGCACCATCAATAGATCGG694570.19117905872973784No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAGTAGATCGGAAGAGCACACGT691070.19021569045072478No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG647100.17811303238552392No Hit
TTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC615870.16951703485593048No Hit
ACCCGCTGAATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT600670.16533326404421675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT589270.16219543593543143Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG587180.16162016744882077No Hit
CTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC565680.15570233373488357No Hit
CGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGAA549580.15127083965142363No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG545130.15004598569667849No Hit
CTGCGGCTTAATTTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC536990.1478054663277739No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA536660.14771463446146693No Hit
ATTGATCATCGACACTTCGAACGCACTGTGGGCACCATCAATAGATCGGA536160.14757701042160795No Hit
TGCGGCTTAATTTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG534460.14710908868608732No Hit
CCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAG527220.14511629258892894No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA520830.14335745735953087Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTNGGCACCATCAATAGATCGGA520390.14323634820445494No Hit
CTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC519840.14308496176061003No Hit
TCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA514910.14172798872760026No Hit
CTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG508750.1400324605565373No Hit
ACCCGCTGAATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC501230.13796259499705787No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATA496030.13653130498252422No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG492950.13568354089699275No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA483090.13296959483097318No Hit
TGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATCAATAGATCG475530.130888719348305No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT464460.12784172310582667No Hit
GGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA461890.12713433554095138No Hit
TCCCACGGCGCGACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA442460.12178626535203046No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA440760.12131834361650984No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC436860.1202448761056096No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG429290.11816124814214427No Hit
CAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCA422010.11615744212179716No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA419430.11544730207612469No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT416530.11464908264494247No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT411090.11315173309127649No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG406740.11195440394450315No Hit
CGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAAG406390.11185806711660184No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA403330.11101580799266475No Hit
ATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGA398600.10971388457559855No Hit
GTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC393320.10826057471468747No Hit
CGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGGA391300.10770457359365711No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG389640.1072476617813252No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATNAATAGATCGGAAGAGCACAC389430.10718985968458442No Hit
GCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA380770.10480621131422646No Hit
CGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA371170.10216382974893358No Hit
ACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCGGAAG370370.10194363128515915No Hit
TTGATCATCGACACTTCGAACGCACTGTANGCACCATCAATAGATCGGAA368680.10147846203043573No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA367860.10125275860506697No Hit
TAGGAATAATGGAATCTGTAGGCATCATCAATAGATCGGAAGAGCACACG365260.10053711359780014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)