FastQCFastQC Report
Sat 1 Jul 2023
SRR2075929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2075929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119173352
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG16878341.4162847412398034No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC16799261.4096490295917832No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG13841881.1614912031676343No Hit
TTTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA11479230.9632379896472156No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG10508080.8817474564280109No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT10394090.8721823986288478No Hit
TCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA10393450.8721286953479332No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG9091740.7629004175362962No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA9037890.7583817899155845No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA8777290.7365144852181383No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA7129230.5982235021802524No Hit
CCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG6701500.5623320891401964No Hit
TTTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG4965050.41662417953973474No Hit
TATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGG4955810.415848838421529No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG4891080.4104172550252677No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG4570950.3835547060889921No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG4352670.3652385308420292No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG4334550.36371805670113233No Hit
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAA4329910.3633287079145009No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA3177620.26663846796891305No Hit
CGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATCGGAAG3144690.26387526634309993No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT3132200.2628272132514994No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC3104190.2604768555977179No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG3026440.25395274608034857No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA2970620.24926881304807133No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA2766760.2321626398492173No Hit
TCGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG2715920.22789658547155744No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG2654100.22270918418070507No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG2643680.22183482763831297No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2625490.22030847970106607No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT2573760.21596774419838424No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGAT2520520.2115003025172943No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA2485410.20855417409086555No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2421260.20317125929293323No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2350900.1972672548473756No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2335050.195937259530973No Hit
TGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATC2314810.19423889327204624No Hit
TCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2230550.18716852069412296No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC2064930.17327111852992103No Hit
TCGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAA2010720.16872228281369478No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATCG2001700.16796540219830353No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1982960.16639290300402054No Hit
TTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1971070.16539519673827752No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1955800.16411387002020383No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATTAAT1862130.1562538913900819No Hit
TCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGA1858110.15591656765683656No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATA1847170.15499857719870128No Hit
TATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCG1749130.14677190585358377No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1706250.14317378603230024No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA1651940.1386165591784311No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1613630.13540191434742893No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG1601950.13442182947073605No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1597210.1340240895464617No Hit
CGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGAA1555500.1305241460356003No Hit
CCTCTAGCATCACCAGTATTAGAGGCTGTAGGCACCATCAATAGATCGGA1552540.13027576836136992No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA1467600.12314833604747477No Hit
TGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG1447210.12143738308208366No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1437710.12064022500600637No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC1430460.12003186752689476No Hit
TGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1390620.11668883828995595No Hit
TGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1388490.1165101070581618No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1375950.1154578583977398No Hit
GCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAAG1341630.11257801995869009No Hit
AGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCAATAGATCGG1332520.1118135873194202No Hit
GTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATC1300810.10915275757285069No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1280040.10740991828441647No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGNAGGCACCATCAATAGATCGG1266260.10625361951722227No Hit
GCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG1259820.1057132302530183No Hit
GGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGAT1253930.10521899224585039No Hit
TCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA1245890.10454434477935974No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGAGGCACCATCAATAGATCGG1242380.10424981584809329No Hit
TTGTCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATC1224280.10273101993472501No Hit
CCCCCGCGGGGGCGCGCCGGCTGTAGGCACCATCAATAGATCGGAAGAGC1223450.10266137349228878No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1214250.10188938882914025No Hit
CCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAGATCGGAAGAG1208790.1014312327138369No Hit
GGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATC1193810.10017424029492768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)