FastQCFastQC Report
Sat 1 Jul 2023
SRR2075935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2075935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35626643
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG16417814.60829553881908No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT6655281.8680626181927946No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA6467591.8153801355912202No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG3321550.9323219142482776No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3166290.8887421697295476No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2748480.771467578351404No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2508400.7040798090350527No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC2246660.6306123201111034No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG1930540.541880973742039No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC1833610.5146738074648234No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA1820480.5109883633998298No Hit
TTTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1760770.4942284346015986No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA1614360.45313278604442186No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA1610770.4521251132193398No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1605370.45060939364957847No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1578650.44310938866735217No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG1538760.43191271206776344No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1520890.4268968030470903No Hit
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAA1503210.42193422490016813No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1296360.3638737447140333No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA1283190.3601770730966709No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA1188860.33369969772341446No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1134310.318388123180733No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1128110.3166478525635996No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATTAAT1120000.31437146632086554No Hit
TTTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1066310.29930128415410906No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA1059020.2972550627349313No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1023150.28718675514838715No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATTAATAGATCGG1016450.28530614012664623No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA986130.2767956554312456No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG951680.2671259259537869No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA932060.2616188115169874No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA928520.2606251731323661No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA807900.22676848896484575No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA789000.22146347047068116No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG778500.21851623797392306No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA760720.2135255909460793No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA754040.21165058970052272No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG721500.2025169758486647No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACTATCAATAGATCGG717720.2014559721498318No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG714630.20058864372935725No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC704790.19782666584668107No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA700360.1965832144218584No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA693470.19464926852636663No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG687180.1928837359164039No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC686380.19265918486903186No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT683980.1919855317269157No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA674900.189436877339243No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG667340.18731486994157714No Hit
CCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCGG643220.18054465586330995No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT614560.17250011459120634No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA606670.17028547988649956No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG593830.1666814355761782No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCA590780.16582533470807226No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA585470.1643348771311403No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCATCATCAATAGATCGG584350.16402050566481943No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA578810.16246548966176802No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACTATCAAT577850.16219602840492156No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATTGG568800.1596557946815253No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGTACCATCAATAGATCGG566820.15910003083927948No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC553660.1554061661100093No Hit
TCTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCG552140.1549795191200024No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA536070.15046884995591642No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT530680.14895593727424727No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA504630.14164399379419498No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG502180.1409563062116181No Hit
TAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATA501870.14086929268076143No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG487910.1369508769041192No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA481290.1350927169871155No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG480050.13474466286368883No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA472860.13272651032543256No Hit
CGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA467600.13125008718896136No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT452440.12699484484126108No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC447560.1256250834522916No Hit
GCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC446950.1254538632786704No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCAA444100.12465390017240749No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTACTGTAGGCACCATC437510.12280416091968026No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC432190.12131089645465613No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATA429540.12056707111023623No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA429040.1204267267056287No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT423190.1187846971717206No Hit
GTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATC417220.11710898498070671No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCATCATCAAT417000.11704723344267941No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCTGTAGGCACCATCAATAGATCGG405840.11391474633183934No Hit
AAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACC403270.11319337609215666No Hit
TGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGATCG395080.11089453474468533No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATC392810.11025737114776714No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATC392080.11005246831704014No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA385290.1081465913024699Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGTACCATCAAT370030.1038632800738481No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA366570.102892096793964No Hit
CGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAAG360970.10132023946235967No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)