FastQCFastQC Report
Sat 1 Jul 2023
SRR2075936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2075936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52332580
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC21343644.078461256830831No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA15064312.8785720100174688No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC14805952.829203146491153No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACC13401632.56085788241283No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACC7124561.3614004889497135No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC6403701.2236545570655986No Hit
TTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGC5685541.0864245561751398No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC4861120.928889804401006No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC4439730.8483682631355075No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA4274400.8167760886239508No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC3554460.6792059554487855No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA3290040.628679113470041No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACC3177970.6072641555222388No Hit
GTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCA3124290.5970066830261378No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCA2970670.567652120342624No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2665940.5094226197141436No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC2599550.4967364498367938No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2493490.47646991606375994No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2435910.4654672099101554No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2248750.42970363777211057No Hit
TTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCAT2242810.4285685895860667No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2053430.3923808075199044No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT1955830.3737308575269937No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1922640.36738872801608485No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG1844600.3524764114438845No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1824990.3487292237455138No Hit
TTTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAG1652490.3157669658174697No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1639320.313250369081746No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1603140.30633689376675105No Hit
CCTCGACACAAGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1550110.29620362688023405No Hit
CCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAG1516550.28979079571463895No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1513270.2891640351001231No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTACTGTAGGCACCATC1459090.2788110198274192No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1318810.2520055384236741No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA1306260.24960741473093817No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCA1305650.24949085254348247No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA1303060.2489959409606788No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1277210.24405637941030237No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATC1267830.24226399692122957No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1235430.2360728249973535No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCAT1234650.23592377826585276No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC1232000.2354174015498567No Hit
TTTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGC1206270.23050077026586496No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1086330.207581969014331No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC1060250.20259845778671717No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT1038040.19835444765001076No Hit
TGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATCAATAGATCGGAAG1036660.1980907495865864No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1012810.19353335914262204No Hit
AATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAA983630.18795748270006946No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC966780.18473769112854746No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA892950.1706298447353446No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCCTGTAGGCACCA868800.16601512862541842No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA833160.1592048395091547No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGCTGTAGGCACCA828060.15823030318780384No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGCTGTAGGCACCAT817960.1563003391004227No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA811340.15503535273819866No Hit
CTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGATC807630.1543264253358042No Hit
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATC807280.1542595453921821No Hit
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG803620.15356017226744792No Hit
ACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAATAGATCGGAAG801420.1531397840503946No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA789670.1508945288002235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCAT778990.14885373509198285No Hit
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG750250.1433619362928409No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC743070.14198994202082144No Hit
AGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA739910.14138611167269033No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAT739690.141344072850985No Hit
CCTGCGGAAGGATCATTAACGGAGCTGTAGGCACCATCAATAGATCGGAA736580.14074979678051416No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGTAC735180.1404822770060257No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA714990.1366242596867955No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCA704050.13453378373472127No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA690320.1319101790892022No Hit
ACGATTAAAGTCCTACGTGATCTGAGTTCTGTAGGCACCATCAATAGATC690040.1318566751343045No Hit
TGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGATCG684930.13088022795742155No Hit
GCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC684750.13084583255784446No Hit
AAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGA673510.12869803093980842No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA673180.12863497270725044No Hit
ACGATTAAAGTCCTACGTGATCTGAGTCTGTAGGCACCATCAATAGATCG664320.12694195470584482No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA663060.1267011869088052No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAA643210.12290813867766504No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG633900.12112913217731669No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG618640.11821316663539233No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT609430.1164532686903646No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA601330.11490547570939558No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCAA601260.11489209972067115No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC595790.11384686174463403No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG594370.11357552025908144No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACT591140.1129583139222259No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAA585140.11181180060298958No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGACTGTAGGCACCA575430.1099563598813588No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTACTGTAGGCACCATCA568070.10854997020976226No Hit
ATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAG559850.1069792469624085No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC558950.10680726996452307No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAAT556540.10634675378129647No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA549600.10502062004204646No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACTA547340.1045887666918008No Hit
ATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAG542260.10361805208151403No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT535920.10240656967418767No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTCTGTAGGCACCATCAATAGAT533640.10197089461287787No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCATC531850.10162885147263903No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)