FastQCFastQC Report
Sat 1 Jul 2023
SRR2075937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2075937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52094596
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC533643510.24374006086927No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC25959034.983056207979806No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACC12483792.396369481394961No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA12326732.366220480911302No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC11701422.246186917353193No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC7114911.3657673820908411No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACC6440871.2363796813013004No Hit
TGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA5610971.0770733302164393No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC5376711.032105134283026No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC4726940.9073762660526248No Hit
TTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCA4726480.9072879651470951No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC4649110.8924361367539926No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT4085430.7842329749519509No Hit
TTTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAG3820350.7333486183480529No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT3633060.6973967127031756No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT3511190.6740027314925333No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3231230.6202620325532422No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC2713440.5208678458702318No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2184730.41937747247334445No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC2154960.41366286821765547No Hit
TCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC2065850.39655744714864477No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGTAC1900070.36473456862972886No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAT1886980.3622218319919402No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1648840.3165088371162337No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1557680.2990099011421454No Hit
TTTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGC1465040.2812268666024399No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGC1394360.2676592405093227No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1300170.2495786703096805No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATCTGTAGGCA1187890.22802557102084062No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA1184440.22736331422936845No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCAC1182020.2268987746828865No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1163500.22334370344286764No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTACTGTAGGCACCATC1100660.2112810319135597No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1057200.20293851592591292No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1057060.2029116417372735No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC1039340.19951013728948008No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT1034130.19851003355511196No Hit
CTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCCTGTAGGCAC1016370.19510085076770725No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCT986250.18931906104041962No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT953700.18307081218174723No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA937690.1799975567523357No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG915700.17577638955103903No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCA908900.17447107181712285No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCAT896410.17207351027350323No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAT887720.17040539099295443No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAA880150.16895226522152126No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCA872580.16749913945008807No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGTAC870150.1670326803187033No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA845030.16221068304282463No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCA774890.1487467145344596No Hit
TGCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCA752090.14437006095603466No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCTGTAGGCAC745460.14309737616546636No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT743900.14279792092062676No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGCACCATCAATAGA734980.14108565118731317No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT734110.140918647300768No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG707000.13571465262922855No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATA706450.1356090754595736No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA702180.1347894127060703No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA701450.13464928300816462No Hit
TTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG661600.1269997371704351No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTTGTAGGCAC654100.12556004849332167No Hit
TCGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCC649570.12469047653234512No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA646470.12409540521247156No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA639450.12274785661069336No Hit
TCACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGAT626670.12029462710489204No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATA604720.11608113824320666No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA597620.11471823296220592No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC594330.11408668952917882No Hit
AGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCCTGTAGGCAC580600.11145109945760977No Hit
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCAT547610.10511838886321338No Hit
TTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCAC547070.1050147312784612No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA538060.10328518528102223No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGCTGTAGGCACCAT537370.1031527339227278No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA530190.10177447196250453No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)