FastQCFastQC Report
Sat 1 Jul 2023
SRR2075938.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2075938.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45019548
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC481366910.692397444772213No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC26569765.901827357307097No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC13350952.965589525687819No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACC11970382.6589294055106905No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC9115302.0247426740046346No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA7722781.7154281513443892No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC5846311.29861588126118No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACC5609521.246018729463921No Hit
GTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCA5454851.2116625426803485No Hit
TTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGC5194731.1538831975834143No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCA4936941.096621405439255No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA4834681.0739068282071602No Hit
CCTCGACACAAGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA4568381.0147547460938524No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACC4386410.9743345268593101No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC3955460.8786094431689986No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3444320.7650720971254531No Hit
TTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCAT3018220.6704243232295446No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC2835750.6298930411296No Hit
CCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC2693250.5982401244899216No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2685710.5965652964796537No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2611100.5799924957043106No Hit
GCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2367410.5258626763644983No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCA2206660.4901559651376331No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATC2166520.48123983830313No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1881690.41797176639801004No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1775580.3944020051023169No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCAC1670420.37104326325088827No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1666620.3701991854738302No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGTAC1631040.36229595197179676No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAT1625890.36115200445815226No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1518080.337204629420091No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATCTGTAGGCA1443740.3206918025920651No Hit
TTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1255180.27880777479151947No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1253950.2785345601426296No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTACTGTAGGCACCATC1230620.27335236684295455No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGC1174390.2608622369998028No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAA1146070.254571636303412No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1114810.2476279859584552No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT1048790.23296324521072492No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCA1038140.2305976061776542No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT1037410.2304354543941667No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA1031120.22903828354740477No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCA1015370.22553980328722983No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC1011460.22467129167978317No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCAC969840.21542641876368904No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA966290.21463787241933216No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAG904280.20086385585212896No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATA875640.1945021749218806No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA872800.19387133784639507No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT867950.192794028051992No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCTGTAGGCAC866650.1925052646019458No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCAT849970.1888002074121224No Hit
CTTAACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCATC834600.18538613492965322No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT756120.16795370757609562No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAT704930.15658309141619992No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGCACCATCAATAGA680450.15114545352609937No Hit
AAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGA679730.15098552299991994No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGCTGTAGGCACCA674310.14978160153895814No Hit
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCAT667170.14819562382101215No Hit
TTTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAG658810.14633865271148436No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGCTGTAGGCACCAT657360.14601657040181745No Hit
AATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAA653500.14515916508091106No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGCA632630.14052340107901573No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC619190.13753803125699973No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA617500.13716263877193968No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAAT616750.13699604447383612No Hit
CTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCCTGTAGGCAC615890.13680501634534403No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGTAC614120.1364118538018196No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAG583760.12966811661458708No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAG575430.1278178092769834No Hit
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG574030.12750683325385676No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGC546700.12143613703096265No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTCTGTAGGC527150.11709357899372956No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG522280.11601182668471038No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTACTGTAGGCACCATCA519990.11550315876116747No Hit
AGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT519970.11549871624655138No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCCTGTAGGCACCA512710.11388608344090882No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA512000.11372837417203743No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGACTGTAGGCACCA506620.11253333774030783No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA502860.11169814499248194No Hit
AACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCA492260.10934361224595147No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG489460.10872166019969813No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAA488330.10847065812388877No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG483710.10744443724757077No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA480550.10674251993822773No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCA477200.10599839874003178No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGATCT468940.10416364020358446TruSeq Adapter, Index 6 (97% over 37bp)
CGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACC467170.10377047766006002No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGA466500.10362165342042085No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC459520.1020712158194036No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA454900.10104499494308561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)