FastQCFastQC Report
Sat 1 Jul 2023
SRR2075939.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2075939.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142211137
Total Bases7.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC87700866.1669473889376185No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC53217453.74214362690877No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA31103992.1871697713801415No Hit
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACC28801982.0252970764167366No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC25859781.8184075133299862No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC18737451.317579649194423No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCATC17288201.2156713155313568No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC16120091.133532178988204No Hit
TTTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAG12555610.8828851428140962No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG12283640.8637607615780472No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC12276010.8632242353846029No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT11865030.8343249516386329No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACC11593720.8152469802699067No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG9890170.6954567840913894No Hit
TTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT9086020.6389105798373583No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA7244110.509391187836435No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC5727730.40276240812278996No Hit
TTTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGC5522260.38831417260942086No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC5469150.3845795846495482No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACC5311550.37349747087670077No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA5205630.3660493903511931No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCA5002080.351736165360945No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG4988010.350746791371199No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC4823220.3391590913164558No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA4737550.3331349499019897No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTCTGTAGGCACCATCAATAGA4585630.3224522422600418No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT4488710.3156370235616638No Hit
ACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCAC4319340.3037272671548924No Hit
TAGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGCTGTAGGCACCAT4310880.30313237703739054No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA4177860.2937786792324148No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCA4062470.28566468742880524No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA4057530.28531731660369186No Hit
ACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCG3950980.27782493575028516No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG3599650.2531201195585688No Hit
CTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCCTGTAGGCAC3594100.2527298547651722No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGCTGTAGGCACCAT3466440.2437530613372425No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAA3419330.24044038126212297No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCA3291110.2314242097649497No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA3278850.23056211132043758No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC3267890.2297914262509553No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT3263690.22949609073162816No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGTAC3243050.22804472760807756No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT3155470.2218862788502985No Hit
AATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAA3143050.2210129295288596No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG3140550.22083713457687917No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAT3119550.2193604569802434No Hit
TGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA2940020.20673626988862343No Hit
ACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCATCAATAGATCGG2916070.20505215424865073No Hit
AACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCAT2785130.19584471784372276No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA2784600.1958074493139029No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA2719140.19120443429124684No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGACTGTAGGCACCATCAATAGA2676820.18822857734412177No Hit
AAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGA2556180.17974541614135328No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAG2544050.17889245903434414No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGCTGTAGGCACCAT2533850.1781752156302639No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA2517520.1770269230039276No Hit
ATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATCTGTAGGCA2470830.17374377648074074No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT2405070.169119666063847No Hit
ATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCTGTAGGCACCATCAATA2357610.16578237469545018No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA2318240.16301395579166209No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATA2247160.15801575371695398No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2246120.1579426230169301No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2239630.15748625932158886No Hit
TTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCA2175840.15300067532685574No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC2169430.15254993706997785No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTACTGTAGGCACCATC2148240.15105989905699158No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA2138880.1504017227567768No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAA2098820.14758478444624207No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGGAAGACCCGCTCCCCGGCGCCT2009690.1413173428182351No Hit
TAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATA1987540.13975979954368833No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGCTGTAGGCAC1979850.1392190542713965No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA1950460.1371524088159143No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1903820.13387277819176707No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATA1898440.13349446745510515No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCCTGTAGGCACCATCA1897630.1334375098906635No Hit
TAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAG1858120.13065924646956448No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAG1855730.13049118649547117No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAT1832420.12885207436320545No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGTAC1827060.12847516998615938No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA1797770.12641555632875645No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT1765530.12414850462801658No Hit
AGGGCGTGAAACCGTTAAGAGGTAAACGGGTGGGGTCCGCCTGTAGGCAC1691770.11896185036478543No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCTGTAGGCACCATCAATAGAT1683010.11834586485304592No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATAGA1659710.11670745590058815No Hit
GCTTAACACAAAGCACCCAACTTACACTTAGGAGATTCTGTAGGCACCAT1658410.11661604252555832No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1646930.11580879210606411No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCAC1610360.11323726354849409No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAAT1603500.11275488220025974No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG1587730.11164596764316706No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGACTGTAGGCACCA1572340.11056377391877545No Hit
CCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAG1569460.11036125813409395No Hit
CTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATAGATC1536230.10802459163236984No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGA1523080.10709991018495266No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC1520950.10695013288586533No Hit
CGGCGGGGCGCGGGACATGTGGCGTACGCTGTAGGCACCATCAATAGATC1515440.10656268081170042No Hit
TCTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA1493530.10502201385254378No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGAT1465990.10308545666152716No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAG1463550.10291388078839422No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1429680.10053221077896311No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)