FastQCFastQC Report
Thu 28 Sept 2023
SRR2078180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151777774
Total Bases7.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC90143435.939171963346887No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGG86530285.701116686557809No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG41326422.7228242258975284Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT35666852.349938931111218Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT25429271.6754277869432979No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACG20480421.3493688476416843No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAA18757631.2358614509658048No Hit
TTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAG16723261.1018253568536327No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA15865661.0453216951251374No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACAC14887910.980901854575888No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA14171640.9337098329034658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT10978190.7233068262023661No Hit
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGA10673650.7032419647951881No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGTC10537410.6942656834590287No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA10123000.666961949250883No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCAC9816440.6467639985285328No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCA9745370.6420814947516623No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAG9101760.599676735277459No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA8405790.5538221953367164No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT8303350.5470728540267036No Hit
AATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTCT8136760.5360969386729838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG8113750.534580906424415No Hit
TGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGA7527920.4959830284505293No Hit
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCAC6801290.4481084298943533No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTAGATCGGAA6626170.4365705086701298No Hit
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA6247840.41164393411119604Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC5557110.3661346357603057No Hit
AAGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAA5167040.3404345619141838Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCT4975010.32778251181889123Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCGGAGAATTTGGACAAACTTGGTCATTAGATCGGAAGAGCACACGTCT4932200.32496194073843776Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG4721230.31106201359890806No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACG4524820.2981213837014107No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAAGATCGGAAGAGCACAC4472610.29468148610481004No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTAGATCGGA4369550.2878912955990513No Hit
ACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAG3913130.25781969895012424No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCG3731930.2458811920643928No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCAC3593160.23673821965527045No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTAGATCGGAAGAGCACACGTCT3338320.21994788248772182Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG3188780.21009532001701384No Hit
TGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGC3185790.20989832147623932No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC3110970.2049687459509058No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCG3052940.2011453930006906No Hit
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAAC2920570.19242408970894515Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGT2748400.1810805315935125No Hit
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTG2441190.16083975510142876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATAGAGGGTGAGAATCCCGTGTGGCGAGGAAGATCGGAAGAGCACACGTC2426440.15986793955747433No Hit
ATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAG2415880.15917218551380258No Hit
TTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAGAT2348940.1547617900892393No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCT2343340.15439282961153455Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC2308470.15209539177982673No Hit
TAAATCAGTTATCGTTTATTTGATAGTTAGATCGGAAGAGCACACGTCTG2207020.14541127741140808Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCA2190960.14435315146999061No Hit
AATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGA2013200.13264129173484912Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGAGAATTTGGACAAACTTGGTCATTAGATCGGAAGAGCACACGTCTG1882820.12405110118428803Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACATGGTATAACTGTGGTAATTCTAGAGCTAATAGATCGGAAGAGCACA1821230.11999319478753193No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTCTG1687750.11119875825824142Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGATATAGTGTAACGGCTATCACATCACGCAGATCGGAAGAGCACACG1673880.11028492221792632No Hit
CTATGCCGACTAGGGATCGGGTGGTGTAGATCGGAAGAGCACACGTCTGA1658250.10925512717033259Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATCAGTTATCGTTTATTTGATAGTTAGATCGGAAGAGCACACGTCTGA1601500.10551610804359274Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAAAAAATTAGAGTGTTCAAAGCAGGCGAGATCGGAAGAGCACACGTCT1598060.10528946089300269Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCTG1556570.10255585906800821Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)