FastQCFastQC Report
Thu 28 Sept 2023
SRR2078181.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2078181.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86251075
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAA24388992.827673742037418No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACG22070022.5588110061236917No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGG22046962.55613741625829No Hit
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC19614882.27416064089636No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCAC17147341.9880726124283088No Hit
CTACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTAGATCGGA16203241.8786131071409833No Hit
TACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTAGATCGGAA14323971.6607294459808182No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACAC13463211.560932428958132No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACA11750431.362351715616298No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAG11736661.3607552137755965No Hit
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT10675921.2377723987787979Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT9327901.0814821728308892No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACG8784101.0184336832903242No Hit
TTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAG8352340.9683751767731591No Hit
TGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGC7910660.9171665396634187No Hit
TTAAATCAGTTATCGTTTATTTGATAGTTAGATCGGAAGAGCACACGTCT7712580.8942010287987715Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACAC7539110.8740888157046158No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT6691360.7758001856788451No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGA6534230.7575824417260887No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG6368030.7383131166770964Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC6298350.730234376788927No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGAT5889910.682879604688985No Hit
TAAATCAGTTATCGTTTATTTGATAGTTAGATCGGAAGAGCACACGTCTG5199180.6027959651517387Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG4764940.5524499259864297No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG4417510.5121686889119933No Hit
TACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATA4193950.4862490119688363No Hit
AAATCAGTTATCGTTTATTTGATAGTTAGATCGGAAGAGCACACGTCTGA3881050.44997120325746665Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAAC3650340.4232225511392177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCAC3625130.4202996890183688No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCA3576980.41471714990218966No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATCGTTTATTTGATAG3428220.3974698286369184No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAGATCGGAAG3314370.38426999315660704No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGTC3311250.3839082585347487No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA3061670.35497180759776037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA2950700.34210588099916434Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTCT2940620.3409371999131605Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGA2895860.3357476993765005No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAGATCGGAAGAGCACACGTCTGA2754950.3194105116950716Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAGATCGGAAGA2753110.3191971810206424No Hit
GTGGTAATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGA2745000.31825690288497854Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGATTAATAGGGACGGTCGGGGGCAGATCGGAAGAGCACACGTCTGAAC2538830.29435343269634606Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT2524620.2927059169987157No Hit
GAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAGATCGGAAGAGCACA2440130.2829100970625584No Hit
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCC2349240.27237225738925575Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCAC2257030.2616813761451669No Hit
ACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAG2252670.26117587519923663No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATAGATCGGAAGAGCACACG2245190.26030863963144807No Hit
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGA2195430.25453943617514335No Hit
ATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAACTCCAG2159310.2503516622836295Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTAATAGGGACGGTCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCC2158460.2502531127872899Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGAA2151080.24939747127789422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA2142070.24835284661669435No Hit
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTG2134260.2474473506562092Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTATGCCGACTAGGGATCGGGTGGTGTAGATCGGAAGAGCACACGTCTGA2120050.24579983495857877Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATTACATGGTATAACTGTG2100060.24348218268583896No Hit
CTATGCCGACTAGGGATCGGGTGGTGAGATCGGAAGAGCACACGTCTGAA2060740.2389233989257525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG1998890.23175247380974673Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGT1945470.22555892781626197No Hit
CTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGAG1930670.22384300717411348No Hit
AGTTATCGTTTATTTGATAGTTAGATCGGAAGAGCACACGTCTGAACTCC1916520.2222024479115188Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGAACTC1855080.21507905843492384Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGGAGAATTTGGACAAACTTGGTCATTAGATCGGAAGAGCACACGTCT1847760.2142303733605639Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACATGGTATAACTGTGGTAATTCTAGAGCTAATACATGCTAGATCGGAAG1799710.20865942830277767No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAGATCGGAAGAGCACACGT1762260.20431745343463836No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATAGATCGGAAGAGCACAC1724810.19997547856649903No Hit
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA1682390.1950572789962328Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCAAGATCGGAAGAGCA1649220.1912115298273094No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACG1620990.18793852714299503No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGT1620570.18788983209774487No Hit
ATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGTCTGAACTCC1600110.18551768775055846Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC1585800.18385857799453514No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGAG1573210.1823988860428696No Hit
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAAC1544800.17910501405344803Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGATTAATAGGGACGGTCGGGGGCAGATCGGAAGAGCACACGTCTGAACT1532350.17766155378353257Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCA1508570.17490448669770203No Hit
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC1508010.17483955997070183Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACT1485360.172213505744711Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCGGAGAATTTGGACAAACTTGGTCATTAGATCGGAAGAGCACACGTCTG1457030.16892890900200375Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAGAT1421600.1648211341134009No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT1361110.1578078881915385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCT1330650.15427633800506255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGAG1313310.15226592827973448No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTG1308510.1517094134768755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC1307250.15156332834112501No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGA1273700.14767352175030862Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG1256890.1457245605344629No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAGATCGGAAGAGCACACGTC1237960.1435298052806878No Hit
TTAATAGGGACGGTCGGGGGCAGATCGGAAGAGCACACGTCTGAACTCCA1232030.14284227761798912Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGGAGAATTTGGACAAACTTGGTCAAGATCGGAAGAGCACACGTCTGA1224010.1419124341348789Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCACGCAGATCGGAAGAGCACACG1223930.14190315888816457No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAGATCGGAAGAGCACACGT1191330.1381234958520807No Hit
ATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCTGAACTCCA1175600.13629975046687823Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGAGATCGGAAG1167510.135361791142893No Hit
GTACAACGGGGTATTGTAAGCAGTAGAGTAGCCAGATCGGAAGAGCACAC1164820.13504991097212413No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGT1156430.1340771694729602No Hit
AATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCC1149450.13326790419713608Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTAGCAGATCGGAAGAGCACACG1135670.1316702429505951No Hit
GCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCA1130730.1310974964659861No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCAGATCGGAAGAGCA1096970.12718334235254458No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAAGATCGGAAGAGCACAC1093730.12680769486061477No Hit
CTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG1089750.12634625133657754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA1039210.12048661422480822Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGAACTCCAG1037210.1202547330569503Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACGTCTGAACTC1025140.11885533020892783Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT1021760.11846345103524797No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAGATCGGAAGAGCACACGTCT1008910.1169736145317609Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCCGACTAGGGATCGGGTGGTGTAGATCGGAAGAGCACACGTCTGAAC1002160.11619101559024046Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAG993330.11516726023414779No Hit
GTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGC983900.11407394052769777No Hit
TACAGTGAAACTGCGAATGGCTCAGATCGGAAGAGCACACGTCTGAACTC983460.11402292667076902Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTAGATCGGAA982940.11396263756712596No Hit
TACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGA976960.11326931287523083No Hit
TGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT972140.11271047926069326No Hit
GCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGTC969660.11242294661254945No Hit
TGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGA948470.10996616563909492Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCCAGT913290.10588737589647433Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTC910390.10555114820308036No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTTGCTCGAATATATTAG903900.10479869381338147No Hit
ATGGCTTAGTGAGGCCTCAGGATCTGCTAGATCGGAAGAGCACACGTCTG893010.10353610085439514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTG875640.10152221291154923Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGCCGACTAGGGATCGGGTGGTGAGATCGGAAGAGCACACGTCTGAACT874160.10135062084733436Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)