FastQCFastQC Report
Sat 23 Sept 2023
SRR20820094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20820094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25403321
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA6414372.5250123792869443No Hit
AAATGAGGCCATTTACCCATAGAGGGTGCCAGGCCCGTCTGTAGGCACCA2979241.1727757957315894No Hit
TCGACATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAA2579661.0154814010341402No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT2441220.9609845893771134No Hit
AACACGGACCAAGGAGTCTAACATCTGTAGGCACCATCAATAGATCGGAA2204940.8679731283952993No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCA2078730.8182906479038705No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA1899960.7479179592306061No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCATCA1866610.7347897544576947No Hit
AAGTGGGTGGTAAACTCCATCTAAAACTCTGTAGGCACCATCAATAGATC1835290.7224606578013953No Hit
AAGTGGGTGGTAAACTCCATCTAAAACTAAATCTGTAGGCACCATCAATA1520580.5985752807674241No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGACTGTAGGCACCATC1484080.5842070806411492No Hit
TTGCCTCTAACTGGAACGTACCTTGAGCATCTGTAGGCACCATCAATAGA1295870.5101183424009798No Hit
AATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCATC1216180.4787484282074773No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGCTGTAGGCACCATCAAT1195410.4705723318616491No Hit
ACTGGAACGTACCTTGAGCATATATGCTGTCTGTAGGCACCATCAATAGA1162690.4576921261594104No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATAGAT1126560.443469576280991No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATAGAT1086760.42780233340357343No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCCTGTAGGCACCATCAATAGA1059250.4169730406508661No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACCAT1054690.4151779997583781No Hit
ATGCCTCTAACTGGAACGTACCTTGAGCATCTGTAGGCACCATCAATAGA1045660.41162334641206955No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCA1035060.40745066363567184No Hit
AAGTTCAAGGCGAAAGCCGAAAATTTTCAACTGTAGGCACCATCAATAGA991300.3902245694568832No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC972570.38285151772085235No Hit
TTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATAGATC951150.37441954931798094No Hit
TGCCTCTAACTGGAACGTACCTTGAGCATCTGTAGGCACCATCAATAGAT931030.366499325029196No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCATC914050.35981515960058924No Hit
ACAGTTCTGACGTGCAAATCGATTGTCAGAATTGAGTCTGTAGGCACCAT884540.3481985682108257No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCATCTGTAGGCACCA865460.34068773921330997No Hit
TTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATAGATCGG838560.33009857254490466No Hit
AAAGAAGCCGTCGAGAGATATCGGAAGAGCTGTAGGCACCATCAATAGAT818120.32205238047419077No Hit
ATGCTGTGACCCGAAAGATGGTGAACTATCTGTAGGCACCATCAATAGAT806910.317639571613491No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCATCAA804250.3165924644262063No Hit
ACAGATCTTGGTGGTAGTAGCAAATCTGTAGGCACCATCAATAGATCGGA734710.28921809081576383No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCATCAAT725290.28550991423522937No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCATCTGTAGGCACCAT725250.2854941682624882No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACCATC684440.26942933957335735No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACCA683260.2689648333774942No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGAATCTGTAGGCACCATCAA616840.24281864564085934No Hit
AACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAATAGATCGG609940.24010246534301558No Hit
ACTGGCACGGACTTGGGGAATCCGACTGTCCTGTAGGCACCATCAATAGA605940.23852786806890328No Hit
AGTCGGTCCTAAGTTCAAGGCGAAAGCCGACTGTAGGCACCATCAATAGA601600.23681943002649142No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACC599520.236000639443953No Hit
AGTAAACAAGTACCGTGAGGGAAAGTTGCTGTAGGCACCATCAATAGATC588820.2317885917357026No Hit
ATGTGCAAGTTATTGGGATATAAACCTAATAGCGTCTGTAGGCACCATCA575930.22671445201987564No Hit
TCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAATAGATC573600.22579724910770524No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCAT567800.22351408306024242No Hit
ATCAGTTATGGTTCCTTAGATCGTTAACCTGTAGGCACCATCAATAGATC562110.22127421843781767No Hit
ATGACGCTACGTCCGTTGGATTATGCCTGCTGTAGGCACCATCAATAGAT556350.21900679836309592No Hit
ATGCTGTGACCCGAAAGATGGTGAACTCTGTAGGCACCATCAATAGATCG539440.21235018838678613No Hit
AAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCAATAGAT522970.20586678411062867No Hit
ACAACCTCAACTCATATGGGACTACCCCCTCTGTAGGCACCATCAATAGA521480.20528024662602187No Hit
AATACAATGACAAAGCCTAGAATCAATTGTCTGTAGGCACCATCAATAGA497090.19567913974712203No Hit
TTGCCTCTAACTGGAACGTACCTTGAGCATATCTGTAGGCACCATCAATA484940.1908963005270059No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGCTGTAGGCACCATCAATAGATC482010.18974290802371863No Hit
GTTTCCAAAGAGTCGTGTTGCTTGATCTGTAGGCACCATCAATAGATCGG479740.1888493240706599No Hit
AAGCGAAAGCATTTGCCAAAGATGTTTTCATTCTGTAGGCACCATCAATA467850.18416883367336107No Hit
ACAGTACGTGAAACTGCTTAGAGGTTCTGTAGGCACCATCAATAGATCGG462350.18200376242145663No Hit
TTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATAGATCG458410.180452784106456No Hit
ATATGCTGTGACCCGAAAGATGGTGAACTATCTGTAGGCACCATCAATAG458390.18044491112008543No Hit
ATGCCTCTAACTGGAACGTACCTTGAGCATATCTGTAGGCACCATCAATA446820.17589038850471558No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCATCA435830.17156418249409203No Hit
AAACAGTACGTGAAACTGCTTAGAGGTTCTGTAGGCACCATCAATAGATC434290.17095796254355877No Hit
GTGCAAGTTATTGGGATATAAACCTAATAGCGTCTGTAGGCACCATCAAT430310.169391238255817No Hit
AGACCGTCGTGAGACAGGTTAGTTTTACCCTACTCTGTAGGCACCATCAA426330.16782451396807527No Hit
ATTTAATACAATGACAAAGCCTAGAATCCTGTAGGCACCATCAATAGATC421580.1659546797050669No Hit
TGCAGATACCATGAAAGGCGTTGGTTGCTTCTGTAGGCACCATCAATAGA412870.16252599414068736No Hit
AACAGTACGTGAAACTGCTTAGAGGTTCTGTAGGCACCATCAATAGATCG412770.16248662920883455No Hit
ATCGACATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCA409890.16135291917147368No Hit
AACACGGACCAAGGAGTCTAACATATCTGTAGGCACCATCAATAGATCGG404870.15937679959246273No Hit
ACGTAGCCAATTGTGGAACTTTCTTGCTCTGTAGGCACCATCAATAGATC401460.15803445541628197No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCATCTGTAGGCACC400880.1578061388115357No Hit
ATGACGCTACGTCCGTTGGATTATGCCTGACTGTAGGCACCATCAATAGA399920.15742823546574877No Hit
ATATGGCAAATGTGAGATGCAGTGTCTGTAGGCACCATCAATAGATCGGA398440.1568456344743272No Hit
AAGCGGTGGATCACTCGGCTCATGGGTCGACTGTAGGCACCATCAATAGA393680.15497186371813354No Hit
ATATGCTGTGACCCGAAAGATGGTGAACTCTGTAGGCACCATCAATAGAT385830.15188171656768812No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGACTGTAGGCACCATCAATA385550.15177149475850027No Hit
TCGCAGAGAGATATGGTAGATGGGCTAGCTGTAGGCACCATCAATAGATC383720.15105111650559389No Hit
ACCCACTCCCAGCTCGGGGAGGTAGTGACGACTGTAGGCACCATCAATAG383600.15100387858737052No Hit
TGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATAGATCGGA381760.15027956384127886No Hit
ATTCAAGGACATTGCCAGGTAGGGAGTTTCTGTAGGCACCATCAATAGAT374640.14747678069335896No Hit
TAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTCTGTAGGCACCATCA372190.14651233986296516No Hit
TAGTCGGTCCTAAGTTCAAGGCGAAAGCCGACTGTAGGCACCATCAATAG370810.14596910380339642No Hit
AGCGAAACCACAGCCAAGGGAACGGGCTTGGAACTGTAGGCACCATCAAT369150.1453156459346398No Hit
ATTCTGGTTGATCCTGCCAGTAGTTATCTGTAGGCACCATCAATAGATCG365640.14393393682660627No Hit
AAGCCCGATGAACCTGAATATCCGTTATGGAACTGTAGGCACCATCAATA361040.1421231499613771No Hit
ACGTAGCCAATTGTGGAACTTTCTTGCTACTGTAGGCACCATCAATAGAT359080.14135159729706206No Hit
AAATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCAT356360.1402808711506657No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTCTGTAGGCACCATC352050.1385842425878097No Hit
AGAGCATGACATATACTGTTGTGTCGATCTGTAGGCACCATCAATAGATC351730.13845827480588072No Hit
TGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCA339440.13362032468117063No Hit
ATAATGTGCCCAGTGGGCCACTTTTGGTCTGTAGGCACCATCAATAGATC338950.13342743651509187No Hit
AAACATGAACCTTATGGGACATGTGCTTTTCTGTAGGCACCATCAATAGA337850.13299442226471098No Hit
ACATTACCGCTAAAGTAGATGATTTATATTCTGTAGGCACCATCAATAGA334750.13177410937727393No Hit
TGCCTCTAACTGGAACGTACCTTGAGCATATCTGTAGGCACCATCAATAG333720.13136865057919003No Hit
ACTGGCACGGACTTGGGGAATCCGACTGTCTCTGTAGGCACCATCAATAG331450.1304750666261313No Hit
AGGGGCGAAAGACCAATCGAACCATCTAGTCTGTAGGCACCATCAATAGA329290.12962478409811062No Hit
ACATTACCGCTAAAGTAGATGATTTATACTGTAGGCACCATCAATAGATC327870.12906580206580076No Hit
TGGATGGCGCTTAAGTTGTATACCTATCTGTAGGCACCATCAATAGATCG326030.12834148731970912No Hit
CAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAATAGATCG323000.12714872988456905No Hit
CATATGGGACTACCCCCTGAATCTGTAGGCACCATCAATAGATCGGAAGA321210.12644409760440375No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTCTGTAGGCACCATCAATAG319990.12596384543579953No Hit
AAGTCACTTTGTCTATATGGCAAATGTGAGATCTGTAGGCACCATCAATA319130.12562530702186536No Hit
AACTGGAACGTACCTTGAGCATATATGCTGTCTGTAGGCACCATCAATAG316250.1244915969845045No Hit
TACTGTTGTGTCGATATTTTCTCCTCGGACCCTGTAGGCACCATCAATAG314120.12365312393603971No Hit
AGATATCTTCAGGATTATGGTGCTGAAGCTTCTGTAGGCACCATCAATAG313390.1233657599335142No Hit
AAGCAGAACTGGCGCTGTGGGATGAACCAAACGTCTGTAGGCACCATCAA312830.12314531631513848No Hit
AGGTCGTATCCGTGCTGGACTGCAATGCTGTAGGCACCATCAATAGATCG311650.12268081011927534No Hit
AAGTGTGAGTCATTAACTCGCATTGATTCTGTAGGCACCATCAATAGATC311320.12255090584416108No Hit
AAGGTCGTATCCGTGCTGGACTGCAATGATCTGTAGGCACCATCAATAGA310510.12223204989615334No Hit
ATGGTGGGGACACGCAAACTTCTCAACAGGCCGTCTGTAGGCACCATCAA310210.12211395510059492No Hit
TGATGTTTCCAAAGAGTCGTGTTGCTTGATCTGTAGGCACCATCAATAGA305750.12035827913995971No Hit
ACTGGCACGGACTTGGGGAATCCGACTGTCTACTGTAGGCACCATCAATA302120.11892933211370277No Hit
AACACGGACCAAGGAGTCTAACATATGTCTGTAGGCACCATCAATAGATC302000.1188820941954794No Hit
AAAGAAGCCGTCGAGAGATATCGGACTGTAGGCACCATCAATAGATCGGA299640.11795308180375313No Hit
AGAAGCCGTCGAGAGATATCGGAAGAGCTGTAGGCACCATCAATAGATCG295810.1164454049137906No Hit
GTTTCCAAAGAGTCGTGTTGCTTGATAGTGCAGCACTCTGTAGGCACCAT286530.11279233923785004No Hit
AATCTTATCTGGTAAAGCGAATGATTAGCTGTAGGCACCATCAATAGATC286010.11258764159221545No Hit
ACGCCTCTAAGGTCGTATCCGTGCTGGACTGCAATGCTGTAGGCACCATC285910.11254827666036264No Hit
ATTAGGCTAAAACCAAGCGATCGCAAGATCGTCTGTAGGCACCATCAATA284870.11213888136909343No Hit
AAATGAGGCCATTTACCCATAGAGGGTGCCAGGCCCGTATCTGTAGGCAC282290.11112326612729098No Hit
AGATGATGTTTCCAAAGAGTCGTGTTGCTTGATCTGTAGGCACCATCAAT278720.10971793806014576No Hit
AATGTTACGGTGCCCAAATTAACAACTCACTGTAGGCACCATCAATAGAT277250.10913927356190947No Hit
TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTCTGTAGGCACCATC276830.1089739408481277No Hit
ACCAATATCCGCAGCTGGTCTCCAAGGTGCTGTAGGCACCATCAATAGAT270820.10660810844377396No Hit
ACAATGACAAAGCCTAGAATCAATTGTCTGTAGGCACCATCAATAGATCG267340.10523820881529623No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA266330.10484062300358286Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAAATTAACGATTTAAGTCCTTCTTCTGTAGGCACCATCAATAGATCGG259500.1021519981580361No Hit
AAGTGGGTGGTAAACTCCATCTAAAACTAAATATCTGTAGGCACCATCAA257640.10141981042557388No Hit
AACAACTCACCTGCCGAAGCAACTAGCCCTTCTGTAGGCACCATCAATAG255320.10050654400658875No Hit
GCAAGTTATTGGGATATAAACCTAATAGCGTCTGTAGGCACCATCAATAG255210.10046324258155066No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)