FastQCFastQC Report
Sat 23 Sept 2023
SRR20820095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR20820095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49759083
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAA12324542.4768422681744355No Hit
TCGACATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAA10146612.0391473050256974No Hit
ATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATAGAT6795721.3657245251083103No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAACTGTAGGCACCATCAAT5740141.1535863713565622No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCATCA5598301.12508102289586No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCA5105221.0259875568848407No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCA3949500.7937244341902362No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATAGAT3918050.7874039800934435No Hit
AAATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCAT3672110.7379778280881905No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCA3617000.7269024632146055No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC3607700.7250334577106254No Hit
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCA3529450.7093076855938041No Hit
ACGTAGCCAATTGTGGAACTTTCTTGCTACTGTAGGCACCATCAATAGAT3269630.6570920931159443No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAAT3037240.6103890620331568No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTACTGTAGGCACCATCAAT3033470.6096314114148768No Hit
ATCGACATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCA3012050.6053266697057098No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCATCAAT2989360.600766698212666No Hit
TTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACC2960990.5950652265838581No Hit
ATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACC2463000.4949850060540706No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCTTGCTGTAGGCACCATCAAT2455070.4933913271673435No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA2414850.4853083807834642No Hit
AGTTACGTAGCCAATTGTGGAACTTTCTTGCTCTGTAGGCACCATCAATA2314610.46516331500723196No Hit
ACTGAAGCTTATCCTTTGCTTGATGATTCGACTGTAGGCACCATCAATAG2241370.45044439424255467No Hit
AACTCTAAGCGGTGGATCACTCGGCTCATGGGTCGACTGTAGGCACCATC2182540.4386214271673777No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCATC2021980.40635395149866405No Hit
TTATGTATGTAAGCGTATTACCGGTGGAGCTGTAGGCACCATCAATAGAT1944810.3908452251823049No Hit
TAGTTACGTAGCCAATTGTGGAACTTTCTTGCTACTGTAGGCACCATCAA1716520.34496616426793875No Hit
AATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCATC1674900.33660186221679367No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTACCTGTAGGCACCATCAATAG1657870.3331793714928388No Hit
ATATGGCAAATGTGAGATGCAGTGTATGGAGCGTCACTGTAGGCACCATC1610170.32359318197242504No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAGAT1485260.2984902274023016No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGCTGTAGGCACCATC1484520.29834151083531824No Hit
TAGTTACGTAGCCAATTGTGGAACTTTCTTGCTCTGTAGGCACCATCAAT1346220.27054759027613107No Hit
TTATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCATCA1331690.26762752038657944No Hit
AACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAATAGATCGG1280660.2573721063147406No Hit
ACAACCTCAACTCATATGGGACTACCCCCTGAATTTCTGTAGGCACCATC1263530.25392951875740954No Hit
TGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATCAATA1260870.2533949429896045No Hit
AAATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCAC1221510.24548482937275995No Hit
AAGTTCAAGGCGAAAGCCGAAAATTTTCAACTGTAGGCACCATCAATAGA1217340.24464679142097534No Hit
ACGTAGCCAATTGTGGAACTTTCTTGCTCTGTAGGCACCATCAATAGATC1201950.241553888764389No Hit
AGCCCGATGAACCTGAATATCCGTTATGGAACTGTAGGCACCATCAATAG1146650.23044033990738938No Hit
ATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACCAT1132680.22763281228474408No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGCTGTAGGCACCATCAA1040300.2090673576118756No Hit
TTTAGTTACGTAGCCAATTGTGGAACTTTCTTGCTACTGTAGGCACCATC1010280.20303428823236153No Hit
ACGATGCCAGCTAGCAATTGGGTGTAGCTACTGTAGGCACCATCAATAGA1002840.20153908382917746No Hit
TATCGACATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATC1000430.20105475014481275No Hit
TTTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCCTGTAGGCACCATC995340.20003182132596778No Hit
TTTTAGTTACGTAGCCAATTGTGGAACTTTCTTGCTACTGTAGGCACCAT960230.19297582312760866No Hit
ACTGTTGTGTCGATATTTTCTCCTCGGACCCTGTAGGCACCATCAATAGA960210.19297180376093345No Hit
ACTGGCACGGACTTGGGGAATCCGACTGTCCTGTAGGCACCATCAATAGA952690.19146052189104854No Hit
TACTGTTGTGTCGATATTTTCTCCTCGGACCTTGCTGTAGGCACCATCAA948250.19056822248914837No Hit
TATACTGTTGTGTCGATATTTTCTCCTCGGACCTTGCTGTAGGCACCATC946650.19024667315513027No Hit
TTATGGCAAATGTGAGATGCAGTGTATGGAGCGTCACTGTAGGCACCATC939500.18880974956873703No Hit
TTCGACATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCA911850.1832529751402372No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTACTGTAGGCACCAT908780.18263600235559002No Hit
AAGTGGAGCCGTACCTGTTGGTTTGTCCCACTGTAGGCACCATCAATAGA907730.18242498560514067No Hit
TTTTAGTTACGTAGCCAATTGTGGAACTTTCTTGCTCTGTAGGCACCATC877480.17634569350886148No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTACCTGTAGGCACCATCAAT846660.17015184946233836No Hit
TATGTATGTAAGCGTATTACCGGTGGAGCTGTAGGCACCATCAATAGATC842800.16937611169401975No Hit
TTTAGTTACGTAGCCAATTGTGGAACTTTCTTGCTCTGTAGGCACCATCA838270.1684657251420811No Hit
TAACCTCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAAT818930.16457899756713765No Hit
AAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCAATAGAT800790.16093343199270774No Hit
TCAACTCATATGGGACTACCCCCTGAATCTGTAGGCACCATCAATAGATC774840.15571830373160214No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAG765700.1538814531610239No Hit
ACCGAAAGGTTGGTGGTTCGTGTCCACCCGGGGGCGCCACTGTAGGCACC763990.15353779731029207No Hit
AATATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACC761670.15307155077596588No Hit
ATGGTGCTGAAGCTTATGTAGCCTTCATTCATGCTGTAGGCACCATCAAT741500.14901801948400054No Hit
TTATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCACTGTAGGCACCATC740560.14882910925026493No Hit
TTATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACC736260.14796494541509134No Hit
GCGTCATCGTGTGAACTGCAGGACACATGACTGTAGGCACCATCAATAGA732770.14726356593026443No Hit
ACCGAAAGGTTGGTGGTTCGGGTCCACCCGGGGGCGCCACTGTAGGCACC726740.1460517268776838No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTACCTGTAGGCACCATCAATA726110.1459251168274142No Hit
TTACTGTTGTGTCGATATTTTCTCCTCGGACCTTGCTGTAGGCACCATCA724690.14563974179347317No Hit
TGCAGATACCATGAAAGGCGTTGGTTGCTTCTGTAGGCACCATCAATAGA719710.14463891949134192No Hit
ACGCGAAGGTCGCGGGTTCGTTCCCCTCATGGGCCACCACTGTAGGCACC698320.1403402068321878No Hit
TTATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCA688730.138412920511417No Hit
AACTCATATGGGACTACCCCCTGAATTCTGTAGGCACCATCAATAGATCG678500.13635701445703893No Hit
ACATTTTGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATAG675600.13577420628913117No Hit
TCGCAGAGAGATATGGTAGATGGGCTAGCTGTAGGCACCATCAATAGATC668930.13443374750294332No Hit
ATGTAAGTGGAGCCGTACCTGTTGGTTTGTCCCACTGTAGGCACCATCAA664400.13352336095100467No Hit
AACGATGCCAGCTAGCAATTGGGTGTAGCTCTGTAGGCACCATCAATAGA656570.13194977889765372No Hit
TTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGCTGTAGGCACCATCA656420.13191963364758952No Hit
TTTGCCAAAGATGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAA640550.12873026619079775No Hit
ATTATATCAGTTATGGTTCCTTAGATCGTTAACCTGTAGGCACCATCAAT640110.1286418401239428No Hit
TTTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGCTGTAGGCACCAT629620.1265336823027868No Hit
AAGCGGTGGATCACTCGGCTCATGGGTCGACTGTAGGCACCATCAATAGA628910.1263909947858163No Hit
GTCTAGGGGTATGATTTCCGCTTGGCTGTAGGCACCATCAATAGATCGGA626780.12596293223490473No Hit
AAGCCCGATGAACCTGAATATCCGTTATGGAACTGTAGGCACCATCAATA613210.12323579194576395No Hit
ACTGGCACGGACTTGGGGAATCCGACTGTCTACTGTAGGCACCATCAATA612130.12301874614530176No Hit
AGAACTGGCACGGACTTGGGGAATCCGACTGTCTACTGTAGGCACCATCA606800.12194758492635403No Hit
TGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACCATC584490.11746398140013954No Hit
TGAACGCATATCGCAGTCCATGCTGCTGTAGGCACCATCAATAGATCGGA582560.11707611251598024No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCACTGTAGGCACCA581690.11690127006560792No Hit
TATGGGCCTCGTGCTCATCCTGGCAACAGGAACGACCCTGTAGGCACCAT574520.11546032711253944No Hit
ATTACCCACTCCCAGCTCGGGGAGGTAGTGACGACTGTAGGCACCATCAA555980.11173437420460501No Hit
TGCTTGGACTACATATGGTTGAGGGTTGTACTGTAGGCACCATCAATAGA545180.10956391619998303No Hit
TTTACCCACTCCCAGCTCGGGGAGGTAGTGACGACTGTAGGCACCATCAA541850.10889469164855792No Hit
ACTGGCACGGACTTGGGGAATCCGACTGTCTCTGTAGGCACCATCAATAG537190.10795817921323027No Hit
TCGACATTTTGAACGCATATCGCAGTCCATGCTCTGTAGGCACCATCAAT535180.10755423286237008No Hit
ATATCAGTTATGGTTCCTTAGATCGTTAACCTGTAGGCACCATCAATAGA534080.10733316769523266No Hit
TAACGATGCCAGCTAGCAATTGGGTGTAGCTACTGTAGGCACCATCAATA531530.10682069844414135No Hit
TAAGCGGTGGATCACTCGGCTCATGGGTCGCTGTAGGCACCATCAATAGA528340.1061796094594428No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)