FastQCFastQC Report
Thu 29 Jun 2023
SRR2086025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2086025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36618005
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC12542813.425312220040387No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT8626672.355854722287574No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG8454252.3087685962138025No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC6967731.9028152953717712No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5702041.5571683929804478No Hit
TAGGAATAATGGAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA4676101.2769947461638067No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA3160240.8630289935238143No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG3158770.8626275516648163No Hit
CTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2808160.7668795719482807No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2292330.6260117119979638No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2258300.6167184695070089No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC2158950.5895870078121406No Hit
TCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2124260.5801135261191864No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA2098160.572985884949221No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1975160.5393958518493839No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1717300.4689769418077255No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA1684730.4600824102787686No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1502980.41044835730400936No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1494150.408036975253021No Hit
CCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1488300.40643940050802874No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT1457970.398156589907069No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1434970.39187552680709936No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC1433310.39142219790510163No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC1410160.3851001713501323No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1379970.3768555933071723No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1323430.3614151016692472No Hit
CCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1285680.3511059654942971No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1264890.34542843063132467No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC1196940.32687198551641466No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1194880.32630942073441743No Hit
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1158260.31630887592046586No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1140070.31134137427749No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1081060.29522635108056816No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1025110.27994698236564225No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCA1022210.2791550222356461No Hit
GTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATAGAT1003480.2740400521546709No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1000440.273209859466675Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA996300.27207926810868044No Hit
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC976650.26671305550370644No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA975340.2663553079967082No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT894210.24419954063581564No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC877950.23975910211383716No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA876610.23939316191583893No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG869830.23754161374984792No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG841410.22978040447588555No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG812680.22193453739492364No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA774180.2114205839449746No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG758290.20708118861199565No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG752020.20536891619300396No Hit
CCCTCCCACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT745660.20363206570101236No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT736530.20113875674002446No Hit
CGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC732080.19992350757503038No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG712370.19454090958805648No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA701460.1915615009610709No Hit
CCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA693200.18930578003908186No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATA685510.18720572024609206No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA683290.186599461112095No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC658800.17991149435912743No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT641340.17514334819715055No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAG610310.16666937480619165No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG605970.16548416550819742No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC590770.16133320206821752No Hit
TCCCACGGCGCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG569330.15547815890024594No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAG561060.15321970708125687No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA548740.1498552419772732No Hit
GGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG546920.14935821872327562No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA539760.1474028964712851No Hit
ATCTCGCTGGGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT529190.1445163383422991No Hit
AATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT528290.1442705576123003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT526830.14387184665030223No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTGTAGGCACCAT524990.14336936160230465No Hit
CTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC522730.14275217888030764No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATC521300.14236166060930955No Hit
CTCGGATAGCCGGTCCCCCTGTAGGTACCATCAATAGATCGGAAGAGCAC518150.1415014280543137No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG509800.13922112905932477No Hit
GTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC492660.1345403716013475No Hit
GCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC490660.13399419220135012No Hit
CCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA470730.12855151448037652No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACTGTAGGCACCATCAA465250.12705498292438377No Hit
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG463110.12647057096638661No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATC460310.12570591980639031No Hit
CCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC453490.12384344805239934No Hit
TCCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGC446770.12200828526840826No Hit
TAGGAATAATGGAATACTGTAGGCACCATTAATAGATCGGAAGAGCACAC442730.12090500288041361No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCTGTAGGCACCATC439180.11993553444541831No Hit
TGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATAGA437170.11938662414842098No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC437150.119381162354421No Hit
GCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA436140.11910534175742234No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATTGGAAGAGCAC425790.11627886336243605No Hit
GATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT423820.11574087665343864No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG422900.11548963412943987No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG414010.11306186669645166No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG411220.11229994643345534No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACTATCAATAGATCGGAAGAGCAC410100.11199408596945681No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAGTATCT405720.11079795308346263TruSeq Adapter, Index 1 (97% over 37bp)
TAGGAATAATGGAATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC398260.10876070392147252No Hit
TCCCACGGCGCGACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA393440.10744441156747889No Hit
CTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA389680.10641759429548386No Hit
TCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC389270.10630562751848442No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA387330.10577583350048697No Hit
AATAATGGAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC369460.10089572056151065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)