FastQCFastQC Report
Fri 30 Jun 2023
SRR2086027.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2086027.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58243116
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA27981884.804323999423382Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC19990803.432302626116364No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC15256352.6194254442018523No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC13103412.2497783257338084No Hit
TAGGAATAATGGAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA11068131.9003327363185718No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG9170421.5745071056981224No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC8005681.3745281073217306No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA6101941.0476671612143829No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5708010.9800317002270277No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA5584160.9587673846296272No Hit
TCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA5138490.8822484703599993No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG4945400.8490960545448839No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA4036280.6930055047192187No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC4010080.6885071190215853No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3601540.6183632070784125No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC3567380.6124981362604295No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3476490.5968928585482961No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA3205230.5503191141078372No Hit
TCCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGC3046430.5230540893450825No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2945250.505682079234909No Hit
TAGGAATAATGGAATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC2772440.4760116199826946No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA2243760.3852403775924351No Hit
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1819000.31231158717538393No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA1777550.30519486629115106No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1749140.300317036609099No Hit
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC1661180.2852148226410139No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1473200.2529397637310476Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1467530.2519662581239644No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCA1441270.2474575707796952No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG1380960.23710269896960867No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1368290.2349273345883486No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1364630.23429893414356473No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1309700.2248677766484884No Hit
TTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1307040.2244110703143012No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA1257580.21591907960418874Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC1205510.20697896726541895No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA1167350.2004271200050492No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT1161010.19933857934386615No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA1112930.19108352650637717No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1048240.17997663449187712No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1030700.17696511979201113Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1024130.17583708948539087Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1003740.1723362465703243No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC973360.16712017949039676No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG944980.16224750063166266No Hit
GTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC916370.15733533212749123No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATTGGAAGAGCAC889790.15277170266783116No Hit
CTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA871300.14959707856289833No Hit
TTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCGGAAG852880.14643447304570725No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAG844630.14501799663328455No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA840400.14429173054546052No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC837020.14371140445164368No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA830200.14254045061737425Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA821350.14102095773859352Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATAGAT819280.14066555092965835No Hit
CCCTCCCACGGCGCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCAC815600.14003371660266253No Hit
CTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA815500.14001654719160286No Hit
ATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA805270.1382601164401987No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA796680.1367852640301731No Hit
CCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA770090.13221991762940705No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA766630.1316258560067425Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGCA760440.130563069462149No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA759840.13046005299579094No Hit
CCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA738000.1267102536203592No Hit
AATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT721990.12396143090970613Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAATAGATCGGAAG716310.12298620836151693No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT710350.12196291146236063No Hit
CCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC697410.11974118967123942No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA692830.11895483064470658No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG691700.11876081629973233No Hit
TACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAG685260.11765510622748962No Hit
TCCCACGGCGCGACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA674090.11573728301212456No Hit
CTCGGATAGCCGGTCCCCCTGTAGGTACCATCAATAGATCGGAAGAGCAC657970.11296957394930587No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATTGGAAGAGCAC651720.11189648575807654No Hit
GGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAA650180.11163207682775764No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC634530.1089450639969194Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGGAATAATGGAATACTGTAGGCACCATTAATAGATCGGAAGAGCACAC624890.10728993277076729No Hit
TTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGA622970.10696028007842164No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC595930.10231767132788706No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)