FastQCFastQC Report
Thu 29 Jun 2023
SRR2086028.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2086028.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52293661
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA20518753.923754735779543Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC12103002.3144296590747393No Hit
TTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGC11480972.1954802514209133No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG7377611.4108038830939758No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC7254261.3872159380847326No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG5589271.0688236189851004No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA4405990.8425476273309686No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA4206010.804305898567706No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA4079590.7801308843150224No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC3802530.7271493193027736No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3773130.7215272229649403No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC3668500.7015190617463176No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA3451280.6599805662869922No Hit
ACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAATAGATCGGAAG3315890.6340902389679697No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACC2898260.5542277868057469No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC2453730.4692213077221731No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2320030.44365415532869273No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2232880.42698865547011516No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC2168120.41460474530555436No Hit
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG2140150.4092561046739489No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1963920.37555603536726945No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1958750.3745673878139838No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACC1892830.36196165344017506No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA1846930.3531842989535577No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC1663600.31812651250406815No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTACTGTAGGCACCATCAATAG1525740.291763852601561No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1512190.28917271636422626No Hit
TAGGAATAATGGAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1508340.2884364894628433No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1433050.27403895091605845No Hit
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC1425740.27264107594226383No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA1361950.2604426567112982No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1330570.25444192939561067No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1274160.24365477108210112No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1207410.23089031766202026Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1194830.22848467235828065Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT1181450.22592604484126672No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG1134030.21685802414942798No Hit
TCCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGC1118180.21382706404892937No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1089940.20842679192034386No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1023440.19571014544191123No Hit
TCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1005710.1923196771402178No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA978620.1871393169432142No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC946910.18107548446455105No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC936860.1791536454102917No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA923750.17664664939025784Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC914450.1748682311609432No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACCA910900.17418937258953815No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG907140.17347035618714857No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT860320.16451707215526562No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA833150.1593214137369346No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC832200.15913974735867126No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC824460.15765964444524164No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA815460.15593859454590492No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG809380.15477592972501963No Hit
TTTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAG804650.15387142238903487No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC778130.14880006201898927No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA765800.1464422236568979No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA753900.14416661323444155Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACC743700.14221609001519323No Hit
TACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGA703940.13461287401545668No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA697500.13338136719859794No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA691760.1322837198183543No Hit
TTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCAT688790.13171577335157314No Hit
TACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAG639770.12234178823318566No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA630990.12066280844249937No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT620420.11864153094961166No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA614880.11758212912268658Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA609210.11649786768610443No Hit
GGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAA601320.1149890806076859No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA592950.11338850420130273No Hit
ACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACT591710.11315138177072744Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA586090.11207668172247492Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC582830.11145327920338184No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA581570.1112123322174747No Hit
CCGAGTGTTACAGCCCCCCCGGCCTGTAGGCACCATCAATAGATCGGAAG576330.11021029872052752No Hit
CCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAG556160.10635323466834729No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGG554990.10612949818143351No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG551550.10547167466435367No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA544750.10417132585152147Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA541590.10356704610908767No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAA532790.10188424176306952No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGCTATCT528990.10115757625001622TruSeq Adapter, Index 5 (97% over 37bp)
GATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGA526710.10072157694218425No Hit
AATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT524580.10031426179934122Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)