FastQCFastQC Report
Thu 29 Jun 2023
SRR2086029.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2086029.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68022183
Total Bases3.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA55536848.164518918776835Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC19982362.937624039498997No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC14795192.1750536880005744No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG13997922.0578463352168512No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG7806851.147691775784379No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG7410111.0893666849827504No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC7313411.0751507342832558No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC5936270.8726961908881989No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC5836290.8579980445496729No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA5417340.7964078424239928No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4698850.6907820056289579No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA4126830.6066888503122577No Hit
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC3600740.5293479040506536No Hit
TCCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGC3571640.525069887862905No Hit
TAGGAATAATGGAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA3548850.5217195102368296No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA3475060.5108715784672774No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA3288490.48344376128005184No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3140780.4617287863284246Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGC3043810.4474731426952293No Hit
TCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2971240.4368045641816582No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2871360.422121118929688No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2804360.41227139093727705No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG2682870.39441104088059037No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC2673380.3930159077664414No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2619380.38507732102628933No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA2444360.359347479336263No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA2408890.35413300393490754Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2302230.3384528250144515No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG2286340.33611682236072904No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2197360.32303579554334505No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2060110.30285855424545843Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA2025890.2978278424260509No Hit
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG1944190.2858170547099319No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCA1884550.27704932668803056No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1810650.26618522372326686No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1714720.25208247139025225No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1700960.250059601880169Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGGAATAATGGAATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1667330.24511562647144097No Hit
GCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1633130.2400878548693446No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1621680.23840458045870125Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1604940.2359436185692541Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT1507760.22165710265429148No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1501350.2207147630060623Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG1482850.21799506199323243No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1458780.21445651045924238No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1422550.20913030680006256No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1326780.19505107620553724No Hit
TACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAG1318130.1937794322184573No Hit
ACCCAACTTACACTTAGGAGATTCTGTAGGCACCATCAATAGATCGGAAG1296220.1905584241540734No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1278880.1880092557452912No Hit
TTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCGGAAG1260320.18528073408052784No Hit
CTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1259650.1851822368006037No Hit
CCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1210560.17796547341034322No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC1210430.1779463619978206No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1205360.17720101690943968Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1196100.17583969629436916No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA1100310.16175752548253267No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1037400.15250907193025545No Hit
GTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1028890.1512580094643537No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG1007890.1481707812876279No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG999190.14689178675727005No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA987430.1451629389783036No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC980630.14416326509250665No Hit
GGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAA935790.13757129788086925No Hit
TTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGA933710.13726551528050782No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG915900.1346472517649132No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC882630.12975620026778617No Hit
GTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATAGAT882490.129735618746608No Hit
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA876860.12890794757351437Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG865710.12726877642253853No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA855970.12583689059199996No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC855570.12577808624577663No Hit
AATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT854090.12556051016475023Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGC825250.12132071680204677No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATTGGAAGAGCAC821880.12082529018511505No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC795400.11693244246512935No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA787830.11581957021285247No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACGATATCT775780.1140480892828741TruSeq Adapter, Index 7 (97% over 35bp)
CCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC771310.11339095071382817No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA765260.1125015349772No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA750730.11036546710063687No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA735690.10815442368263894No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG717810.10552586940645525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)