FastQCFastQC Report
Thu 29 Jun 2023
SRR2086030.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2086030.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65099755
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA42551456.536345643697122Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG27330404.198233925765158No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC26778894.113516249024285No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT21454023.2955607897449077No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG19279542.961538027293651No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC16226542.4925654482109803No Hit
TAGGAATAATGGAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA12189681.872461731998838No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC11418261.7539635901855544No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC7392191.1355173302879558No Hit
TCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA6836391.0501406648919032No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG6013600.9237515563614641No Hit
CTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA4607600.7077753211206401No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA4131850.6346951689756744No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA3713930.5704983067908627No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3302100.5072369319976703No Hit
CCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3277800.5035041990557414No Hit
CCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3236180.4971109338276311No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA2784550.42773586475095027No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2680760.4117926403870491No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG2628260.40372809390757314No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC2450710.3764545657660309No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2262390.34752665351812767Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG2257050.3467063739333581No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2097080.3221333167843719No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2058100.3161455830363724No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1874970.2880149088118688No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1849440.2840932350667065No Hit
TAGGAATAATGGAATAGGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1826630.28058938163438557No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAG1806530.27750181241081473No Hit
CCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1805620.2773620269385038No Hit
TCCCACGGCGCGACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1793250.27546186617753016No Hit
TCCCACGGCGCGACCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1745830.2681776605764492No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1704210.26178439534833886No Hit
CCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1690100.259616952475474No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA1653470.2539902031889367No Hit
CGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1565170.24042640406250376No Hit
TCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1563680.24019752455289575No Hit
TTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1557200.23920212910171473No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1551740.23836341626784927Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1422110.21845089893195452No Hit
CTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC1421910.21842017685012793No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT1409720.21654766596279815No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGATCT1377170.21154764714552304TruSeq Adapter, Index 6 (97% over 37bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1369590.21038328024429584Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC1315140.20201919346701075No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1302270.20004222750147063Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGC1279640.19656602394279363No Hit
TAGGAATAATGGAATACTGTAGGCACCATTAATAGATCGGAAGAGCACAC1247790.19167353241191154No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1227140.18850147746331763Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGAATAATGGAATACTGTAGGCATCATCAATAGATCGGAAGAGCACAC1214060.18649225331185962No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC1186230.18221727562569168No Hit
TCCCACGGCGCGACCGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1175970.18064123282798836No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1168060.1794261744917473No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG1159660.17813584705503116No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1138260.1748485842995876No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1128470.17334473839417675Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGAATAATGGAATACTGTAGGCACTATCAATAGATCGGAAGAGCACAC1103460.16950294206176353No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCA1080940.16604363564809116No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA1076720.1653953997215504No Hit
GGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1074270.16501905421917487No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1067280.16394531745933605No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG1060710.16293609707133308No Hit
GTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1030670.15832164038098148No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1025340.15750289690030322No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1024350.15735082259526167No Hit
CCCTCCCACGGCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT981640.15079012202119654No Hit
AATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT980430.15060425342614578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG975400.1498315930682074No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC973520.1495428054990376No Hit
CCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA961820.14774556371218295Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA961730.14773173877536097No Hit
TCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA949530.1458576917839399No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC945160.14518641429602921Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA929000.14270407008444194No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC904740.13897748155887837No Hit
TAGGAATAATGGAATACTGTAGGTACCATCAATAGATCGGAAGAGCACAC856950.13163644010641823No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA847990.13026009084058765Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATAGAT847000.1301080165355461No Hit
GCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG830710.12760570297077153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCTGTAGGCA822160.12629233397268544No Hit
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG817610.12559340661113086No Hit
GCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA800080.12290061613903155No Hit
TTCTGATCGAGGCCCTGTAGGCACTATCAATAGATCGGAAGAGCACACGT779160.11968708637997177No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT778950.11965482819405387No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA773180.11876849613335719No Hit
CTCGGATAGCCGGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC766820.1177915339312721No Hit
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT765870.11764560404259586No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA754400.11588369264984176No Hit
TTCTGATCGAGGCCCTGTAGGCATCATCAATAGATCGGAAGAGCACACGT743090.11414635892254894No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA737970.11335987362778861No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC718710.11040133714788941No Hit
CCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC718660.11039365662743278No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA686150.10539978222652298No Hit
TCGGGCCGATCGCACCTGTAGGTACCATCAATAGATCGGAAGAGCACACG683450.1049850341218642No Hit
CTCGGATAGCCGGTCCCCCTGTAGGTACCATCAATAGATCGGAAGAGCAC681260.10464862732586319No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC675700.10379455345108442No Hit
CCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC657590.10101266894168802No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATA651170.10002649011505496No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)