FastQCFastQC Report
Thu 29 Jun 2023
SRR2086031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2086031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54718534
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC39894527.290860533653917No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG9108821.6646681360286444No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6738261.231440155176672Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC5267810.9627103679349304No Hit
CTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA5263490.9619208730994147No Hit
TCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA5133060.9380843426835961No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC4401660.8044184809483382No Hit
GCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC4238990.7746899798156143No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA3961000.7238863526570357No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCCTGTAGGCACCATCAATA3754120.6860783222006642No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3746900.6847588424061215No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA3507100.6409345688976243No Hit
GTGCATTTATCAGATCAAAACCACTGTAGGCACCATCAATAGATCGGAAG3487080.6372758451459976No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC3304700.6039452738262323No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3136470.5732006635996498No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC3077610.5624437964657459No Hit
CCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA2963250.5415441137366728No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA2916720.5330405964458039No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2883960.5270535939431418No Hit
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG2691310.49184614485468486No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGG2516740.4599428778556092No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCTGTAGGCACCATCAATAG2424680.44311859670801856No Hit
CCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC2366330.43245493382552974No Hit
GCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA2333090.426380209674477No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2100900.38394668979984004No Hit
GTGCATTTATCAGATCAAAACCAACTGTAGGCACCATCAATAGATCGGAA2078450.37984387520323554No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG2071910.37864866774391287No Hit
TCCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGC1966650.35941204126557924No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG1964690.3590538445346507No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGAT1956770.35760643733620495No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA1880810.34372448647838405No Hit
CCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1862450.34036913342744163No Hit
GATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCA1825610.333636496913459No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1786360.3264634246231816No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1729480.31606840928888924No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1699530.31059494393618076No Hit
CTCGGATAGCCGGTCCCCCTGTAGGTACCATCAATAGATCGGAAGAGCAC1654790.3024185552924353No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGA1596520.29176951268467827No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA1588090.2902289012348174No Hit
ATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1466290.2679695329556892No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATC1455660.2660268639507045No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1425630.26053877832326433No Hit
TTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCGGAAG1425190.26045836681223955No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA1408510.2574100395306643No Hit
CTCGGATAGCCGGTCCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1408190.25735155843173724No Hit
GTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATAGAT1365310.24951509117550555No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACTATCAATAGATCGGAAGAGCAC1282460.2343739691564105No Hit
GTGAAATACCACTACTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1276420.23327013841416147No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1250870.22860078817170063No Hit
CCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATAGATCGGAAGAG1242030.22698524781383944No Hit
ATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1233140.2253605697842709No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1132720.20700846992720967No Hit
CCCCGCCCCTTGCCTCTCGGCTGTAGGCACCATCAATAGATCGGAAGAGC1128010.20614770125237641No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGA1074040.19628449841145235No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCATCATCAATAGATCGGAAGAGCAC984250.17987506755937577No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG969730.1772214876955585No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC952570.17408543876559268No Hit
ATGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAAT926680.1693539523555218No Hit
GCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGA901240.16470470499081719No Hit
CCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA887730.1622357060954886No Hit
CCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGCA881730.16113918549060544No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA868610.15874146043459425No Hit
TCGCCCGTCACGCACCGCACGTTCTGTAGGCACCATCAATAGATCGGAAG862790.15767783544785757No Hit
TCGCCCGTCACGCACCGCACGTTCCTGTAGGCACCATCAATAGATCGGAA853120.1559106097396542No Hit
TACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAG846430.15468798926520946No Hit
TTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGGAAGA836540.15288055780149373No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC776400.1418897662718815No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGG770980.1408992426588037No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA765960.13998182041938478No Hit
TGCGTGCATTTATCAGATCAAAACCAACCCGGCTGTAGGCACCATCAATA761130.13909912133245383No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA758680.1386513754187932No Hit
GGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAG734970.13431829149516322No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATC718160.1312462062671489No Hit
CGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC715870.13082770090295182No Hit
CCAAGCGCCCGGCCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC695800.12715983947961762No Hit
CTCGGATAGCCGGTCCCCCGCTGTAGGCACCATCAATAGATCGGAAGAGC682360.12470363332467935No Hit
TAGGAATAATGGAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA681440.12453550016526392No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC675630.12347370271286874Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTGCATTTATCAGATCAAAACCAACCCTGTAGGCACCATCAATAGATCGG669190.1222967705969608No Hit
CTCGGATAGCCGGTCCCCCTGTAGGCACCATTAATAGATCGGAAGAGCAC666550.1218143015308122No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG659190.12046923625548887No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA658270.12030110309607343No Hit
ATGCGTGCATTTATCAGATCAAAACCACTGTAGGCACCATCAATAGATCG654970.1196980167633877No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA640990.11714312375400993Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAG628060.11478012185048671No Hit
CTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC623760.11399428208365378No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCCTGTAGGCACCATCAATAGA623520.11395042125945845No Hit
GGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGC608270.11116343138871375No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG604060.11039403943095406No Hit
CCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATAGATCGGAAGA601130.10985857186890279No Hit
AATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT598400.10935965499368093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC595500.10882967003465406No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG589680.1077660450479174No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGCATCT589250.10768746107123411TruSeq Adapter, Index 11 (97% over 36bp)
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCG583720.10667683458040012No Hit
GGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAA581610.10629122483434955No Hit
TACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGA578950.105805100699518No Hit
TGTGCATTTATCAGATCAAAACCACTGTAGGCACCATCAATAGATCGGAA567340.10368333332906908No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC562290.10276042848662577No Hit
CCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC560280.1023930940839899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)