FastQCFastQC Report
Sat 1 Jul 2023
SRR2096966.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2096966.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25807053
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG22564398.743497368723194No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT15871166.149931183541182No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1948310.7549525317749377Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGAGCACA1882480.7294440012193566No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAGATCGGAAGAGCACAC1790270.6937134588749828No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT1687490.6538871369776317No Hit
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1389880.5385659493937568Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG1366110.5293552890366832No Hit
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC1330600.5155954846917236Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC1322670.5125226812995657Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC1308160.5069001873247597Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCTG1261060.48864936263741543Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC1080790.4187963654741981No Hit
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG1069320.41435184404821424Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT950830.36843803901204836No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT949270.367833553098837Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT932100.3611803331438115Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG921540.35708842850053435No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG878120.34026357058281703Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT857360.3322192580454653Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC846060.32784061008438276No Hit
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTCC838040.32473293250492413Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA814450.3155920205224517Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACAC792090.30692772243308836No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG768300.29770931225661446Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT759070.29413277060344706Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG737880.2859218369489922No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT719450.2787803783717575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAAGATCGGAAGAGCACA717840.27815651791004575No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG677290.26244375907624945TruSeq Adapter, Index 3 (100% over 49bp)
GTTGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT630180.24418905947920516No Hit
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC548790.2126511694303104Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG497310.1927031342943342No Hit
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA484120.1875921283999378Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCCGGGCGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTA483510.18735575890823333TruSeq Adapter, Index 3 (100% over 36bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC467720.18123727649181795No Hit
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAAC451110.17480105147999658Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACG450330.17449880852339086No Hit
GGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG435710.16883369054188402Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACGT426980.16545089437372024No Hit
CGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAA418100.16200997456005534Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC414220.16050650959642698Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT412800.1599562724190166No Hit
CGGGCGGCGGCGGTCGGCGGGCGGCGGAGATCGGAAGAGCACACGTCTGA394070.15269856655077974Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAACTC392880.15223745229647107Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC391980.15188871042346447Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT381100.14767280866978497Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC380160.1473085671579781Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG365180.14150395242726863Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCAGATCGGAAGAGCACACGTC362070.14029885551054588No Hit
GTGTGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT339840.13168493124728345No Hit
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG336930.1305573325245622Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCACACGTCTG324780.12584931723897338Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGAGATCGGAAGAGCACACGTCT323520.12536107861676418Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCA322220.12485734035575467Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGTCT321010.12438847628204586Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGGCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTG317050.1228540120408169Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAAC314450.1218465355187979Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGCGTCCGCTGGGGGCGGGGAGCGGTAGATCGGAAGAGCACACGTCTG310900.12047094257527195Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCCA310260.12022294835446729Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG305140.11823899458802986Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGCGTCCGCTGGGGGCGGGGAGCGGAGATCGGAAGAGCACACGTCTGA293350.11367047605164371Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT291200.112837370466128Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG284200.11012493367607684TruSeq Adapter, Index 3 (100% over 38bp)
CTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG280920.10885396329445288TruSeq Adapter, Index 3 (100% over 37bp)
CGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACGTCTG276600.1071800023040213Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGTCTCCGCCCCCCGGCCCCGCGTCCAGATCGGAAGAGCACACGTCTGA272900.10574628571499427Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA270030.1046341866310733Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGTCGGGGCGGCGGCGGCGGCAGATCGGAAGAGCACACGTCTGAACTC269980.10461481208257294Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA259770.10065852927879833Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)